LOCUS Exported 10445 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pJRL2 PHO5pr VYFP (EB#1547) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10445) AUTHORS Raser JM, O'Shea EK TITLE Control of stochasticity in eukaryotic gene expression. JOURNAL Science. 2004 Jun 18. 304(5678):1811-4. PUBMED 15166317 REFERENCE 2 (bases 1 to 10445) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10445 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(24..44) /label=T3 /note="T3 promoter, forward primer" promoter complement(24..42) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(63..79) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(63..79) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(76..98) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 87..103 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(111..141) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 156..177 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(294..311) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(465..1053) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(545..564) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1224..2084) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1847..1866 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2085..2189) /gene="bla" /label=AmpR promoter rep_origin complement(2215..2670) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2352..2373) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2564..2583 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2797..2819 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 2811..2828 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 2812..2828 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 2838..2857 /label=T7 /note="T7 promoter, forward primer" promoter 2838..2856 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 6555..6770 /gene="S. cerevisiae URA3" /label=URA3 promoter CDS 6771..7574 /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /label=URA3 /note="yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" CDS complement(8213..9028) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 8936..8955 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 9020..9039 /label=pENTR-R /note="pENTR vectors, reverse primer" ORIGIN 1 gatctggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca 61 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 121 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 181 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 241 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 301 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 361 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 421 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 481 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 541 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 601 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 661 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 721 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 781 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 841 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 901 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 961 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 1021 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 1081 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 1141 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 1201 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 1261 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 1321 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 1381 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 1441 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 1501 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 1561 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 1621 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 1681 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 1741 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 1801 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 1861 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 1921 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 1981 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 2041 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 2101 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 2161 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg 2221 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 2281 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 2341 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 2401 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 2461 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 2521 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 2581 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 2641 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt 2701 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg 2761 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga 2821 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggagctc tgatacacgg 2881 tccaatggat aaacattttt tatcaacact atgatatata aatataatag tttttcgtat 2941 atatattcct ttttttggtc aatttttgaa attttcgtag aaaagggaga gacaaaacac 3001 attatatcaa tgaaaacgta caaaaagtag ataaagtcag tgcttaaaca cgtcttttcc 3061 ttaaaaatac tttattattt ttattttatt gagagggtgg tttaaaaata gaaatagaga 3121 gagaggtaca tacataaaca tacgcgcaca aaagcagaga gcggccgctt atttgtacaa 3181 ttcatccata ccatgggtaa taccagcagc agtaacaaat tctaacaaga ccatgtggtc 3241 tctcttttcg tttggatctt tggataaggc agattgatag gataagtaat ggttgtctgg 3301 taacaagact ggaccatcac caattggagt attttgttga taatggtcag ctaattgaac 3361 accaccatct tcaatgttgt gtctaatttt gaagttagct ttgataccat tcttttgttt 3421 gtcagcagtg atgtaaacat tgtgagagtt atagttgtat tccaatttgt gacctaaaat 3481 gttaccatct tctttaaaat caataccttt taattcgatt ctattaacta aggtatcacc 3541 ttcaaacttg acttcagctc tggtcttgta gttaccgtca tctttgaaaa aaatagttct 3601 ttcttgaaca taaccttctg gcatggcaga cttgaaaaag tcatgttgtt tcatatgatc 3661 tgggtatcta gcaaaacaca ttaaaccata acctaaagta gtgactaagg ttggccatgg 3721 aactggcaat ttaccagtag tacaaattaa ttttaaggtc aatttaccgt aagtagcatc 3781 accttcacct tcaccggaga cagaaaattt gtgaccatta acatcaccat ctaattcaac 3841 caaaattggg acaacaccag tgaataattc ttcaccttta gacattttgt tgaattctgg 3901 taatctcgaa tttgcttgct ctatttgttg ttgttcttat tctcatgaga gatgaagcca 3961 tactaacctc gacttagcaa aacatcagcg cttatatacg tttcatttcg acaattcaaa 4021 gatgtttacc cttatcccaa atgggtatat gccttgccaa gtaaggtgac caatttgata 4081 atttggcatg tgcgatctct tcgaaaacag ggaccagaat cataaattta gtctgtgcta 4141 gtcccacgtg tgagtgccaa ggttgtatcg attcatttag agattgccta ttcaattaac 4201 tcttttaatc acttttactt tttttggcat tgtgcagttg cgttctatgc gaaaacgtgc 4261 taatttaata tatatttctt tgtgcagaca aagaaaaagc gctatgaacc ttttaccttc 4321 gtttgaagtt tatagacgac gtccgcttac atgagaaaac attaaagcag cgcacataag 4381 atgacttcca aatacgttga catatttgcg cattcttgtt gaatacaact ttcggatcct 4441 tgtaatgccg gtctccggcg tcctgtaaag agagcgtgcg acacgccgct attagcgtgg 4501 ggactgacat caggtcaaca ttattattgt agcgagctac tttcggtcag taaatagaac 4561 atatgtagag atacaagcga ttattagaag gagcggacct acaacaaagt tgggtcacgt 4621 ttctcgcaat tattacttgg atgccctcct agctaatgcc aatgtgcagt agtaacttat 4681 cagtccgatg tgtaaaaaat agaactaatt ttttgcgaaa agatggacaa aaaccatctc 4741 tggtcaattt tctcactaaa gagggacgga gtctcttgtt ttcttctatt agtgcacagg 4801 gaatgttttg attttccctt tttttttctt tcgcttcttc aacagtggta aaaataaaaa 4861 ttattggcga tgggataacc aaggaacatt gtatttagtc gacctcgaga tgctgtcgcc 4921 gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata 4981 aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca 5041 tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag 5101 gatgtaaagg aatacaggta agcaaattga taggcgcgcc ggtgagagcg ccggaaccgg 5161 cttttcatat agaatagaga agcgttcatg actaaatgct tgcatcacaa tacttgaagt 5221 tgacaatatt atttaaggac ctattgtttt ttccaatagg tggttagcaa tcgtcttact 5281 ttctaacttt tcttaccttt tacatttcag caatatatat atatatattt caaggatata 5341 ccattctaag atccgtcgat cttccagtgg tgcatgaacg catgagaaag cccccggaag 5401 atcatcttcc gggggctttt tttttggcgc gcgatacaga ccggttcaga caggataaag 5461 aggaacgcag aatgttagac aacacccgct tacgcatagc tattcagaaa tcaggccgtt 5521 taagcgatga ttcacgagaa ttgctggccc gctgcggcat aaaaattaat ttacacactc 5581 agcgcctgat tgcgatggcg gaaaacatgc cgattgatat cctgcgcgtg cgtgatgatg 5641 acattccggg tctggtaatg gatggcgtgg tcgatctcgg tattatcggc gaaaacgtgc 5701 tggaagaaga gctactcaac cgccgcgcac agggcgaaga tccacgctat ttaaccctgc 5761 gccgtcttga cttcggcggc tgccgtttat cgctggcaac accggttgac gaagcctggg 5821 acggcccggc cgcgctggac ggtaaacgta tcgctacctc atatccgcac ctcctcaaac 5881 gctacctcga ccagaaaggc gtctctttta aatcgtgtct gttaaatggt tctgtcgaag 5941 tcgcgccgcg cgcggggctg gccgacgcta tctgcgattt ggtctctacc ggcgcgacgc 6001 ttgaagctaa cggcctgcgt gaagtcgaag ttatctaccg ctctaaagcc tgtctgattc 6061 agcgcgacgg tgagatggca cagagcaagc aagagctgat cgataaattg ctgacccgta 6121 ttcagggcgt gattcaggcg cgcgaatcga aatacatcat gatgcacgcg ccaagtgaac 6181 gcctggaaga ggttatcgcc ctgctgccag gcgccgaaag gccgacaatt ctgccgctgg 6241 caggcgagca acagcgcgtg gcgatgcaca tggtcagcag cgaaacgttg ttctgggaaa 6301 ccatggagaa actgaaagcg cttggcgcca gctcgattct ggtactgccg atcgagaaga 6361 tgatggagtg atctgacgcc tgatggcgct gcgcttatca ggcctacgta atgcgttgat 6421 attttgggtt ctgtaggccg gataaggcgg aaccctgtga tggagtaaag accatgagct 6481 tcaataccct gattgactgg aaggatctga attaattctc atgtttgaca gcttatcatc 6541 gataagcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc 6601 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca 6661 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat 6721 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag 6781 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata 6841 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat 6901 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata 6961 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt 7021 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc 7081 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg 7141 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac 7201 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat 7261 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg 7321 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg 7381 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg 7441 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg 7501 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg 7561 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta 7621 gagcttcaat ttaattatat cagttattac ccgggaatct cggtcgtaat gatttttata 7681 atgacgaaaa aaaaaaaatt ggaaagaaaa agctttaatg cggtagttta tcacagttaa 7741 attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg 7801 gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct 7861 tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctaggggggg 7921 ggggggcgct gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc 7981 gccccatcat ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg 8041 gaccagttgg tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga 8101 tgcgtgatct gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccgccgtccc 8161 gtcaagtcag cgtaatgctc tgccagtgtt acaaccaatt aaccaattct gattagaaaa 8221 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt 8281 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg 8341 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt 8401 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg 8461 gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac 8521 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag 8581 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc 8641 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta 8701 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag 8761 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga 8821 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg 8881 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc 8941 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgagc 9001 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag 9061 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt 9121 gagacacaac gtggctttcc cccccccccc ctagcgctat atgcgttgat gcaatttcta 9181 tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct 9241 tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatc 9301 ttccagtggt gcatgaacgc atgagaaagc ccccggaaga tcatcttccg ggggcttttt 9361 ttttggcgcg cgatacagac cggttcagac aggataaaga ggaacgcaga atgttagaca 9421 acacccgctt acgcatagct attcagaaat caggccgttt aagcgatgat tcacgagaat 9481 tgctggcccg ctgcggcata aaaattaatt tacacactca gcgcctgatt gcgatggcgg 9541 aaaacatgcc gattgatatc ctgcgcgtgc gtgatgatga cattccgggt ctggtaatgg 9601 atggcgtggt cgatctcggt attatcggcg aaaacgtgct ggaagaagag ctactcaacc 9661 gccgcgcaca gggcgaagat ccacgctatt taaccctgcg ccgtcttgac ttcggcggct 9721 gccgtttatc gctggcaaca ccggttgacg aagcctggga cggcccggcc gcgctggacg 9781 gtaaacgtat cgctacctca tatccgcacc tcctcaaacg ctacctcgac cagaaaggcg 9841 tctcttttaa atcgtgtctg ttaaatggtt ctgtcgaagt cgcgccgcgc gcggggctgg 9901 ccgacgctat ctgcgatttg gtctctaccg gcgcgacgct tgaagctaac ggcctgcgtg 9961 aagtcgaagt tatctaccgc tctaaagcct gtctgattca gcgcgacggt gagatggcac 10021 agagcaagca agagctgatc gataaattgc tgacccgtat tcagggcgtg attcaggcgc 10081 gcgaatcgaa atacatcatg atgcacgcgc caagtgaacg cctggaagag gttatcgccc 10141 tgctgccagg cgccgaaagg ccgacaattc tgccgctggc aggcgagcaa cagcgcgtgg 10201 cgatgcacat ggtcagcagc gaaacgttgt tctgggaaac catggagaaa ctgaaagcgc 10261 ttggcgccag ctcgattctg gtactgccga tcgagaagat gatggagtga tctgacgcct 10321 gatggcgctg cgcttatcag gcctacgtaa tgcgttgata ttttgggttc tgtaggccgg 10381 ataaggcgga accctgtgat ggagtaaaga ccatgagctt caataccctg attgactgga 10441 aggaa //