LOCUS Exported 5401 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAJ08 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5401) TITLE A series of vector constructs allowing cell- and tissue-specific transgene expression and co-transformation in Strongyloides stercoralis REFERENCE 2 (bases 1 to 5401) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5401 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(366..377) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(1339..1367) /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" primer_bind 2115..2135 /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" primer_bind complement(2841..2863) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 2963..2982 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(3060..3078) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3146..3250 /gene="bla" /label=AmpR promoter CDS 3251..4111 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3469..3488) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4282..4870 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4771..4790 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5024..5041 /label=L4440 /note="L4440 vector, forward primer" protein_bind 5158..5179 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 5194..5224 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5232..5248 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 5237..5259 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 5256..5272 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 5256..5272 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 aagcttgatt atcgtagtga gaggacgtgt agagataata aatttcccaa cttaatctat 61 tataggaact tagttaaaaa tgattgtatt ggtcctcttt gtgaaagtgt ggaaaagagg 121 tacctcgtag tttttttctt ctcaattctc atcatcaccc tgtgtaacat cacatctcat 181 atagagaaat cgcgactttt attgcttagc atatactttg taagttgttt tcactgaggc 241 tttgtccatc acacgctaca cattatcgct gttattttta ttatttatat ttattagtaa 301 taatattaaa taaacacata ggaattttgt ctttgttggc ccttccagcc taaatagtta 361 taaaatctac cttctattat acatacgcat atgttattta aaaagttgaa gtgatttctc 421 ttctacagaa actacacatt tatcttctta ttttcttcca aacatatagc aataataatt 481 taaaactttt tcttactcct cccagagttt gttaagttga tcatatttaa aattcttctg 541 tcttctacaa caaataatat atttattata acaccaacta cctcgttcca agtattacgt 601 aggagcatat acataccaca atgcttagga aatggggcgg ttatgatata acattatttc 661 aatgtaacat tcttttttac cacatatatt ttctactgaa ttctgaaaac tcaatttcaa 721 acctcctata tatatatatt gttaggcact tctaagatta taattcattt aactctctta 781 aatttatcta ttttaaattt ttcctttttt gtattattta tactattata tcgaaacttt 841 attattacat gtactattta acttttattt tcttatataa taataatact aaataataac 901 tgataatcgt atctgtagag attattgaat tgtgattagt aatgtaaagt aaatctatat 961 taagatatct caaagatgca ctcaagttat ttgaaaaatt tcttatacat agattaatac 1021 ttatattaag aaactttaaa aaaaagtttt aaataaaaaa aaaaattcac aattgtacta 1081 ttttatcttg tcgttcagtt tatcaccgta gtatttttat ggctagtaat attcatcatt 1141 tttagataaa ctaacttgct atagaaaatg tgtgacgaag aggttgccgc tgcaggtcga 1201 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat 1261 aacatagaac attttcagga ggacccttgg agggtaccgg tagaaaaaat gagtaaagga 1321 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg 1381 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt 1441 aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa catatatata 1501 ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt ctgttatggt 1561 gttcaatgct tctcgagata cccagatcat atgaaacggc atgacttttt caagagtgcc 1621 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag 1681 acacgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt ttcaggtgct 1741 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt 1801 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta 1861 tacatcatgg cagacaaaca aaagaatgga atcaaagttg taagtttaaa catgatttta 1921 ctaactaact aatctgattt aaattttcag aacttcaaaa ttagacacaa cattgaagat 1981 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc 2041 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa 2101 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg 2161 gatgaactat acaaatagca ttcgtagaat tcctaggaat ttattttgat aagtctcgcg 2221 tattttattt gtaataatnt tataataaga tcaatttaca tttatactga taaaattgac 2281 acaattacac tttttattat aaaatatttt aaaatatttc tatcaaaagg tttatttttg 2341 ttttaaatat aatggttttt tttgtaaaaa atacttattt cgtttgataa tctttattca 2401 gagatttgaa taattttaaa taaagtagtt tagttttcat ataaaagtta atggtatata 2461 taaatattta aatagtaaag attgaatcag tattcaaaat gacacctaaa agttattcta 2521 tttctagcta taacaataat aataaagata attatgatac agatttagaa aaaaaaataa 2581 ggttaaatta attaattcta tttttaatta aaatgattta agggagttaa atgaaacaga 2641 aacagattgg gtctctatat atttagttta tgtaatgtta ttttttggtg gaatacaaat 2701 aacggtatat tttacttcaa tgtggccata cttatcaaca ataaatagta aggcaaatgt 2761 agatacttta gggtgggttg tagcactagt cggccgtacg ggccctttcg tctcgcgcgt 2821 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 2881 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 2941 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 3001 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg 3061 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 3121 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 3181 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 3241 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 3301 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 3361 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 3421 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 3481 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 3541 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 3601 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 3661 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 3721 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 3781 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 3841 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 3901 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 3961 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 4021 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 4081 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 4141 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 4201 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 4261 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 4321 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 4381 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 4441 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 4501 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 4561 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 4621 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga 4681 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 4741 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 4801 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 4861 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 4921 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 4981 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 5041 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 5101 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 5161 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 5221 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 5281 cgccaagctg taagtttaaa catgatctta ctaactaact attctcattt aaattttcag 5341 agcttaaaaa tggctgaaat cactcacaac gatggatacg ctaacaactt ggaaatgaaa 5401 t //