LOCUS Exported 899 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION retroviral expression of both TCRalpha and TCRbeta using a single vector. ACCESSION . VERSION . KEYWORDS murine TCR OTII-2A.pMIG II SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 899) AUTHORS Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA TITLE Generation of T-cell receptor retrogenic mice. JOURNAL Nat Protoc. 2006;1(1):406-17. PUBMED 17406263 REFERENCE 2 (bases 1 to 899) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..899 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 129..848 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 129..845 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(174..195) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(435..454) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 782..803 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag 61 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg 121 ataataccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 181 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 241 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 301 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc 361 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 421 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 481 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 541 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc 601 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 661 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 721 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 781 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 841 acaagtaaag cggccgcgac tctagagtcg acctgcaggc atgcaagctt caggtagcc //