LOCUS Exported 22128 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION BAC recombineering generated construct, used as a reporter for mouse Oct4 expression. ACCESSION . VERSION . KEYWORDS mouse deltaPE Oct4-GFP transgenic reporter SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 22128) AUTHORS Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D, Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, Novershtern N, Hanna JH TITLE Derivation of novel human ground state naive pluripotent stem cells. JOURNAL Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30. PUBMED 24172903 REFERENCE 2 (bases 1 to 22128) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..22128 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(3..458) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(140..161) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 352..371 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 585..607 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 599..616 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 600..616 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 623..641 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 674..692 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS complement(1054..1083) /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS complement(1057..1086) /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS complement(1060..1089) /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS complement(1063..1092) /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS complement(1066..1095) /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS complement(1069..1098) /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS complement(1072..1101) /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS complement(1075..1104) /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" regulatory 8268..8277 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 8274..8993 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 8274..8990 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(8319..8340) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(8580..8599) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 8927..8948 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 9120..9241 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9157..9176) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9211..9230 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" regulatory 15425..15434 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter 17357..17375 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter complement(17391..17409) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 17979..18478 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(18014..18037) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 18411..18430 /label=mPGK-F /note="Mouse PGK promoter, forward primer" promoter 18495..18542 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 18561..19364 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(18624..18643) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 19234..19253 /label=Neo-F /note="Neomycin resistance gene, forward primer" promoter complement(19815..19833) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind 19844..19860 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 19844..19860 /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(19894..19910) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(19895..19911) /label=pBluescriptKS /note="For pBluescript vector" primer_bind complement(19940..19960) /label=T3 /note="T3 promoter, forward primer" promoter complement(19940..19958) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(19979..19995) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(19979..19995) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(19992..20014) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 20003..20019 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(20027..20057) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 20072..20093 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(20210..20227) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(20381..20969) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(20461..20480) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(21140..22000) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 21763..21782 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(22001..22105) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctccaccg 661 cggtggcggc cgctaatacg actcactata gggcgaagaa ttcggatttt tttttaagat 721 ttatttattt attatatata agtacactgt agttgtctcc agacgcacca gaagagggcg 781 tcagatctca ttacgggtgg ttgtgagcca ccatgtggtt gctgggattt gaacttcgga 841 cctttggaag agcagtcggg tgctcttacc cactgagcca tctcaccagc ccctggatta 901 taaattctta tttgtacagt ttttgttgtt ggttttgttt gtttgtttgt ttcaacactt 961 gggattgaat ccagggtcat tggagtgttt ggcctgggtt ctgctgctga gccacactcc 1021 atatacttgc atggattgtg tcttttattc tatgtggtgg tggtggtggt ggtggtggtg 1081 gtggtggtgg tggtggtggt ggtggtagag ggaactcacc cagcccacct cctctggttt 1141 cactcctcca cacacaccct tcatccctgt ccttaccccc tgccagtaag aaactccaca 1201 ggatgtctct ctaggagtct gagaaaaaag cactaagaga acaagctgtt tgttttttgg 1261 ggtttttttt gggggggtgg gggggggagt tagaactcag gaaatcagaa cagggctagg 1321 tgtggtaatg aatgacatag atctagtcac tcaaaggctg tgtacctctg aagttgagac 1381 tagcctggtt tatataaaaa gtaacctggt ctatataaaa aattctaagt tacgccgggc 1441 gatgatggca catgcctttg atcctagcac ttgggaggca gaggcaggcg gatttcgaag 1501 tttgaggcca gcctggtcta caaagtgagt tccaggacag ccagggctac acagagaaac 1561 cctgccttga aaacaaacaa acaaacaaac aaaaaaccaa aaacaaccaa aacaaacaaa 1621 caaaaaaacc ccaaaaccaa aacaaaacaa aacaaaaagt tctaagctat gtagaactct 1681 tggggcactc gtgatcaaag tctaagcatt gaaaatctct aaatgccagg cttggtggcg 1741 cacgcactta ataccagcac ttgggaggca gaggcaggca gatttctgag ttcgaggcca 1801 acctggtcta caaagtgagt tccatgacag ccagggctac acagagaaac cctgtctcga 1861 aagacaaaaa aaaaaaaaaa aaaaaaaaaa aaaaatctct aaacaagccc tatcaatctt 1921 cccttggggg tatggactgt tatctcttca tcataagagg aaagcgaata ggtggtgtga 1981 aggaggtctt tggtggtgca ggagggagcc actggctgca ggggacacag ggtacagcat 2041 ggctccttag ttggacatct gcttgacaga atgagtgtct atctatctgt ggacttggga 2101 atcaacccca ggtattccag gaggtaagaa gaaccactct ctgaaccagg gtggtctttt 2161 gagccaccag aaaaaacatc caccatgtaa ttgtaaccaa gtaaccagtc ataaccccct 2221 gcaacagcgc ccaggagact tttgaatctg aaacaagcag aaccacagag agggggagga 2281 aaggttatcc tgacccagag aggcaaatcc tctctggaac cccgggagct aggtcagatg 2341 ctaagggcat ttgtggggag ggctgggaga gaagggccct aggctagcag caggtcctcc 2401 ttccctttaa cctctggcca tgttgggtag ggactctcct tgacaaacct gataaccagg 2461 agttcatttc cgagcaccca cacagtggaa gaagctagcc catcgcctaa agttgccctt 2521 ttgttctctt tttctctctc tcacacaaat ttttaaattt tattatttat ttattgacat 2581 aatttctcta tgtatcccag gctgtctcag aactcacttt gtagaccagg ctggcctcaa 2641 acttagagat gctcctacct ctgcctcctg agtactagga ttaaggatat ccatcacaac 2701 acctaactgt aataaaaatt tcaatttttt tttctctctt agcctcagct gcaatcacct 2761 cttttagcca gtatacctgc aggagctcca gtccataggc gggctctcct aatgcctgaa 2821 tttgactctc aaagtgcatt cacttcctct cattacagct cttctgcaga ctcttttata 2881 tcaggaatct caaaacttgg gttgggcatg gtactcgcct ttaatcccag gaggtggagg 2941 ccagcctggt gtacaaagtg agttctaggt caggcaggac tgtaatgtct actccttaca 3001 tgaacaggat agggcctggc ggggagagag gggggaagct aggtgggagt aagggtggaa 3061 acgggcaatc gtgtcaatgg aagaagtaaa tgatcaagac aataggtctg ttcacagcat 3121 tagaaccacc caaatggtca tttggcatcg gagtcctctc cctcctccca atcccccccc 3181 ccatttgctt atctatttat ttttatttta tgtatgcaag catttttctt ttttcttttt 3241 ctttctgggt ttttcgagac agggtttctc tgtgtagccc tggctgtcct ggaactcact 3301 ctgtagacca ggctagcttc gaactcagaa atctgcctgc ctctgcctcc caagtgctgg 3361 aatcaaaggc aagggccacc acacctggcg caagcatttt tcaaagatgt atttgtttat 3421 atatgtgagt acattatctc tctcttcagg cacaccagga gagagagaga gagagagaga 3481 gagagagaga gagagagaga gagagagaga gagagaatca gatcctgtca cagatggttg 3541 tgagtcacca agtgggagct gggatttgaa ctcaggacct ctggaagagc tgtcagtgca 3601 cttaacctgt gagccatctc tccagtcctt ttcctctctg tttttttttt tttttttttt 3661 ttttttttga cacagtttaa ccatgtagct taggctaatc ttcagtttgc tgtgcctcag 3721 cttctgagtg tccacttggg attgcaggag tttgccgcct tgctcagctt acagcttggg 3781 atgctcttct cacagacatc cacgtactag tactgaaaga caaagacaaa caccggtttg 3841 cttgcaaact gctcaagtct tgtctagttt gattaggctt gagaggagac cttgccagct 3901 ttcccaagaa gactgtgtgg aggttggcgg tagaaggtgg ggtacagaag acttgctttc 3961 atctcacaag gtgccccacc catctctcta acgcccaatt tttctttaca gaggcacagg 4021 gacaactccc taccccgacc catgtggagt gagaagggca ggaggatgca tgggaatagg 4081 gctgtagttt tcttttcaag ttggaagaaa atggagagtt aggccaggga aggtagtgac 4141 acccccctgg ggtgtcacac acacacacac acacacacac acacacacac acacatacac 4201 acacagggaa ttctagcact ggtggtgtga gcaagtaggt agctatatgt agtaatataa 4261 caagatttat tttgctgaga gtcatcacat aaggttcaca ctctaagcca gccaggtggt 4321 ggtggtggca catgccttta atcccagcac ttcggagaca gagacaggag gatttctgag 4381 ttcgaggcca gcctggtcta caaagtgagt tccaggatag ccagggctac acagagaaac 4441 cctgtctcga gagaaaaaaa aaaaaaaaag gttcacactc tgtaaattcc taactaatgt 4501 ctcattcctg tctccccaca gctgctcacc tatgtgacat attttagcag aaggtcaggt 4561 ccactctctt aatctcttaa gagtgtctgt gatttgaggg acaggatcct agcggggaga 4621 ctctccacct acaaggcaga tcaaggtggc tggttatctt gaatgagtga tgtcgtgggg 4681 ttctggttat caggggcagc cttgggtgta gtggtgaagc catttctgca agtctaaaag 4741 gcatttggac actgctcatc agaactggga aggcaagagg atcagaagtt caagggcctt 4801 cttggctacg tagagtttag gggcagcctc tgctacatgt aaatttgtct cagaagaaaa 4861 aaggaaacgg gcagtagtgg cgaatgccta taatcccagc acttgggaag cagagggcag 4921 gctgatttct gagctcaagg ccagcctggt ctacagagtg agttccagga cagccagggc 4981 tacacagaga aaccctgtct cagaaagaaa aaaaaaaaaa gggagaaata gaactcattt 5041 atttcaaagg gagcaggcac attccgcaag ccttctgttc tgctggtgac ctattcctac 5101 cttcagcttc tagatctttg ttttttcttt taatttttat tgtattgtgt gtgtgtgtgt 5161 atctgagtgc aatatcaata tcgaacctct ggagatcaaa tgcttctttg aaatggaccc 5221 cagggatcaa atccatggtc gttaggctca gcaagtgtgg ttacttgctg agctgtcctc 5281 aggttcctct caggcctccc ctcaagtctc cccctccaac cccccccccc tcctgccccc 5341 tgcaagtctt tttgattagg caagaaaacc aagacaagga aagggagatg cagttagcta 5401 aggaatctat gccagccaga gaaactacct cctcccttcc agaacatctg gatttgggaa 5461 gagacgttgc tggtcccagg gcggctgggg gttggggttg ggggaggggg atgctaacca 5521 gcaaggaagc tgttcctggc tggggcaggc ctgactgagc tcatgtcgct gaaactcctc 5581 atttctccct atggcttcat agggagaccc agcctggatg ctaacacgag tgatttccct 5641 gctctagtct agtgtcctcc gtgagtccat ttaactgatc acccagtctg tgaggaggtg 5701 gctgaactca cagtaagaaa gctgtggggg tcaacgccta ttgtttgttt gttttgtttt 5761 agacaaggtc tcctgctgag gctggctcaa gctggcctgg aggactcttg tgtttaaggc 5821 tggccttaaa ttctctttaa aagaaaatca tgtgtatata tgtgtgtcca tgtaactgca 5881 gatgaccaca gcagccaaga gatttctgtt ctcctagctg taaaccaccc aatatgggtg 5941 ctaggaacag aattttaaaa gggtcctctg aaagagcaat gtacactcaa atgctgagtt 6001 ctttcccagc ccctagcctt ggacctttgt tcttatcact tccaacgccc aagggcaggc 6061 attataggtg tggcattccg catctggctt cccaggatac cttttcatgc tggtggacca 6121 tctctggctg gggacgtgtg ggcttctctg ctgtctttgg ttctccagac agaactccga 6181 gacagatctt gacttggttc taaaatacag gtggtttgtg gcaagttaac gaattttagc 6241 tcaaatttgg ggtatttaag ataccatggt gactcttttt tgtttgtttt tctttctttc 6301 tttttttttt ttaaaaagat ttatttatta ttgtacgtaa gtacttcaga caccagaaga 6361 gggtgacaga tttttttttt ttttcgagac aggatttctc tgtgaagccc tggctgtcct 6421 ggaactcact ctgtagacca ggctggcctg gaactcagaa atccacctgc ctctgcctcc 6481 caagtgctgg gattaaagcc atgagttggg accgcacccg gcccaaagtg actcttaaag 6541 ggggcagagt ggcacatatt ttcaatccta gcactcagga agcaaaggct ggtggatctc 6601 tgtgagttca aggccagcct ggtctacaga gtgagttcca agccatctaa ggctatgtag 6661 ggaacccttg aatcaaaccc aaaagttagt ctgatgattt tctaagaccc aggaggcaag 6721 aaactggatc agatgagcca acaggtctgc tgtcccatct ccagggccac caggctcaca 6781 gctcgggacc aggctagggc acatctgttt caagctagtt ctaagaagac ttgggacttc 6841 agacaaagtt gctgttaagg actgtattat actctaggca cgcttagggc taacctggtt 6901 gcaaagccag tcactaggca gttaaaggac tcagaatatg tctcttgtcc tggccagtga 6961 gtcaccaaaa gagaaatcac aatccataag acaaggttgg tattgaatac agacaggact 7021 gctgggctgc aggcatactt gaactgtggt ggagagtgct gtctaggcct tagaggctgg 7081 ccctgggagg aactgggtgt ggggaggttg tagcccgacc ctgcccctcc ccccagggag 7141 gttgagagtt ctgggcagac ggcagatgca taacaaaggt gcatgatagc tctgccctgg 7201 gggcagagaa gatggttggg gaggggtccc tctcgtccta gcccttcctt aatctgctat 7261 tgaggaagct ttgtgaactt ggcggcttcc aagtcgctgc ctttatttag gtcttccaac 7321 taacctatgg cactgttcca caatgaatgt atagaaattg ggaggtgagc atgacagagt 7381 ggaggaaacg gaagattcat ggagagggcc agagagatgg cccctcagcc accctggggg 7441 atgacttgga cccatgtggt agaaggaggg gacttccaca catgtgctat gtgtagctgt 7501 gtgtaggtac atacacaccc ttaaaataaa acgcaatttt tttttcaaag tctcagggtg 7561 aatttggtga agtcgatgaa gctgaggcag gagaattatc aggagttcaa gggcagcttg 7621 ttttatagag aaaggttcca tctctacctg atgaagacta ccatcaagag acacccccgc 7681 cccccagggc acctagagcc actgacccta gccaacagct caggcgggct gggcccaggc 7741 tcagaactct gtcctggcta tgtacactgt ggggtgctct gggctttttg aggctgtgtg 7801 attcaccctg gggccttcgt tcagagcatg gtgtaggagc agacagacaa acaccatccc 7861 ttgcagacag gcactctgag ggctattctc ttgcaaagat aactaagcac caggccagta 7921 atgggatcgt gacccaaggc aggggtgaga ggaccttgaa ggttgaaaat gaaggcctcc 7981 tggggtcccg tcctaagggt tgtcctgtcc agacgtcccc aacctccgtc tggaagacac 8041 aggcagatag cgctcgcctc agtttctccc acccccacag ctctgctcct ccacccaccc 8101 agggggcggg gccagaggtc aaggctagag ggtgggattg gggagggaga ggtgaaaccg 8161 tccctaggtg agccgtctat ccaccaggcc cccggctcgg ggtgcccacc ttccccatga 8221 cgcgaattct gcagtcgacg gtaccgcgga cccgggatcc accggtcgcc accatggtga 8281 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 8341 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc 8401 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 8461 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg 8521 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg 8581 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 8641 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 8701 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca 8761 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact 8821 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 8881 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 8941 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc 9001 ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa 9061 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta 9121 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 9181 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 9241 aaggctggac acctggcttc agacttcgcc ttctcacccc caccaggtgg gggtgatggg 9301 tcagcagggc tggagccggg ctgggtggat cctcgaacct ggctaagctt ccaagggcct 9361 ccaggtgggc ctggaatcgg accaggctca gaggtattgg ggatctcccc atgtccgccc 9421 gcatacgagt tctgcggagg gatggcatac tgtggacctc aggttggact gggcctagtc 9481 ccccaagttg gcgtggagac tttgcagcct gagggccagg caggagcacg agtggaaagc 9541 aactcagagg gaacctcctc tgagccctgt gccgaccgcc ccaatgccgt gaagttggag 9601 aaggtggaac caactcccga ggaggtaagt gaagggactt ggctgggctg gcagaggcag 9661 cagtgaaggg aattgggaac atgtagggta gccaccctgc ctgccaaagg tggtgatggc 9721 tgccgggcct cctgagaagc acgacgcagt gtggactaga acccagaatt gcaagaatca 9781 gaaaccggcc tggattgttt cggcctggcc cttgtcatgt aggtcaccta ggcctggcct 9841 gtgtcccgac acttgcttca tgccatcact gtctgtacac cagtgatgcg tgaaaatcag 9901 ccccccccca aaaaaaaaaa catatcagcc cctctgggga cttggatcac agtcggaccc 9961 aggaacttgg ccttaaggtt aggcatggct gggggggtaa aaaatggtgc ttatcctgga 10021 gttattgtta ctgaagaggt tgggtgtgac tggctgctga taggagctct tgtttgggcc 10081 atgtgtggag tagggctcac cttcagtcaa gtttacggcc tgtctacttt agcctcagac 10141 tccatgagtc acctttacac gagcagaccc ttgtagtgcc tgaggtgcag atctgatcga 10201 tttcagcctt tctacctttc cttgtaaaca agaaagggac acccttgggt aggggagttt 10261 tatctccagg ccatcttaag atcattctgt gagtgcacgg gccttgctta gtgtctgatg 10321 gcctacagcc agcactctgg agcaagtgta agcaattagc cttaagaaca aggtgcgagt 10381 ggataccgat gcccgccggg agttccgaca gcttagcgat tgttgtagca ggagtcccct 10441 ccctaagtgc cagtttctgt gttatctcag gtcctgtatg ccgccgggag tcccctagga 10501 aggcattaat agtttatctc acatcttaaa tggcccttaa tgaagcaaga gatttgaacc 10561 ttagttaagc taatcccaaa tcctcaaaat aggatttaga aaagccaaag acactgctga 10621 gggcgattac aagttttggt cttttgagga gcagttggag atgaaagtct gtctgaagcc 10681 gagagaatcc ttttccattg aaatggcatt gaggtgtgcc tcactggctg ctgcttctgt 10741 ctgtgccctg ggttggccag cctttgtgga gcacctcagc cctccatcct ggacctttgc 10801 tccaacaacc tgctcctctt ccgccctcaa ggctgacttg catctcccca gatgactgcc 10861 tccatttctg tcttctgtta gagacagaaa agcctgagaa accgacagcc attttggggg 10921 gggggggtcc ggttcacacg ctgcaactta gaaagcacac tcaactggcc atctgttata 10981 ccctccccac ctggtcccaa ccatcactgt gtactactga gaagaaggca gccttagcca 11041 caccctcgag tgcccctgcc gttctattgc tcatacatcg attgatatcc ctgtttcaac 11101 tttgaaaaaa aaaaattttt ttttttttgt ggtgtgtgca tgcctgctac tgtacacctg 11161 tgggcgtcag aggtggtcct ctgcaccctc cggccagtac cgcatccagg gtgagtcaga 11221 tgatttcctg tggtttgggc ctcaaggctt ctcacctcca gaggcttcta gcctgctgcc 11281 ttgctttctc tgtcgcactc tagtacagca ggagttttct tcgcactccg gagtgttgtc 11341 agctcctggg gcatggacat ttggctactt agagtgtgct gtgtaggttt tcatttagag 11401 ctgaacagag ggatggatct tattacccca gcccttgaga cactgaggca ggagagcttc 11461 ctagtgagtc cctgtttcaa tatcttcact aatactgtgt catactttgg gactttcttt 11521 cttcctttct ttcttttgat tttttttttt tttatatgag tacagtgtac ctgtcttcag 11581 acacacacca gaagagggca tcagatccta ctacagaagg ttgtgagcca ccttgtggtt 11641 gctgggaatt gaactccgga tctccggaag agaagtccgt ataccaactt ctgtattagt 11701 cagggttctc tagagtcaca gaacttatgg acagtctcta gatagtaaag gaatttattg 11761 atgacttaca gtcggcagcc caattcccaa caatggttca gtcgcagctg tgaatggaag 11821 tccaaggatc tagcagttac ttagtctcac gcagcaagca ggcgaaggag caagagctag 11881 agcttaactg ctgagccatg tgtttcttga gtaaagggat tacatgctcg ttcgtctggt 11941 caattctgca gccttaaaac ttcttcagaa tagggtgaca ttttgtcctc agtggggcgg 12001 ttttgagtaa tctgtgagca gataggaact tgctggggta ctgcacagaa ctctgggtag 12061 tgtggtactg tagatggcta ggttctgggg ggggaaagag ccatctatgt cacctaggaa 12121 tagagtgaat aacatttata taatcagacc agcccttgag gaggctgaga tcttttcatg 12181 gggcacccta gggtcacagt cccagctggt gtgactctga caagtctgcc tttctcacta 12241 cagtcccagg acatgaaagc cctgcagaag gagctagaac agtttgccaa gctgctgaag 12301 cagaagagga tcaccttggg gtacacccag gccgacgtgg ggctcaccct gggcgttctc 12361 tttggtgggt ctcccccagc atgttctgat ctcacggctc ttaatgtagg cgcaaggggg 12421 tggggcatct taggagctgc ttctccacag gtaagggagg attagacgct tgtagcttga 12481 actgtcagag gtgggggctt gggctccctt cttgctgcct cactcactct gtttgatcgg 12541 cctttcagga aaggtgttca gccagaccac catctgtcgc ttcgaggcct tgcagctcag 12601 ccttaagaac atgtgtaagc tgcggcccct gctggagaag tgggtggagg aagccgacaa 12661 caatgagaac cttcaggagg tgaggagtgg caggatgtgt gcaatgtctg ccaggcacag 12721 tcccttctgc tgcttccatt cctggcttga aactcctccc tctccaaccg gagctcgcag 12781 gagaagttct gtgtccttat tctgctgcta tgaattggaa tccagagcct taacatttgc 12841 taatcaatca ggctctctcc ttctgagtca ccctctgccc ccaccagcct gacaatggtc 12901 cctccccaga accccgtcta gtgctggtga aggctcagac ctaggtctac cagccccttc 12961 cagagcccct ttcagtaacc cctggctctg gggccacatc cagtcaatgc tcccttagca 13021 caatccctta gcggtttgtt cttcagtccc atctcaaggt ggggctgttg ccaagtcaaa 13081 tactaaagtt gctcttgtcg cccccatctt cccctgccca gatatgcaaa tcggagaccc 13141 tggtgcaggc ccggaagaga aagcgaacta gcattgagaa ccgtgtgagg tggagtctgg 13201 agaccatgtt tctgaagtgc ccgaagccct ccctacagca gatcactcac atcgccaatc 13261 agcttgggct agagaaggat gtgagtgcca agatcctgcc ctgtggtacc tggatgtttc 13321 cctgttccca ttccccaccc cccccacccc cccaccccca ccgccgccac cgctgactgc 13381 agcatcccag agcttatgat ctgatgtcca tctctgtgcc catcctaggt ggttcgagta 13441 tggttctgta accggcgcca gaagggcaaa agatcaagta ttgagtattc ccaacgagaa 13501 gagtatgagg ctacagggac acctttccca gggggggctg tatcctttcc tctgccccca 13561 ggtccccact ttggcacccc aggctatgga agcccccact tcaccacact ctactcagtc 13621 ccttttcctg agggcgaggc ctttccctct gttcccgtca ctgctctggg ctctcccatg 13681 cattcaaact gaggcaccag ccctccctgg ggatgctgtg agccaaggca agggaggtag 13741 acaagagaac ctggagcttt ggggttaaat tcttttactg aggagggatt aaaagcacaa 13801 caggggtggg gggtgggatg gggaaagaag ctcagtgatg ctgttgatca ggagcctggc 13861 ctgtctgtca ctcatcattt tgttcttaaa taaagactgg gacacacagt agatagctga 13921 attttgtttt ccttcagttc ctagagagcc tgcggttgga gaaagccagt aatggattct 13981 caaaccccag gtgatcttca aaacaggcgc cattgaaacc attggagttc cacaaaatgc 14041 ccagggatag ttggggttgg agcccaacct atagaggaag gcattgcata ttcgccatcc 14101 tagaggcggt aagtctctgc tagctgatgg acatcacctc atagccattg tctggcagcc 14161 gccttctttc ctcttgtcac tctgggagtt ctggtgggct tatactttaa aaaaaagagt 14221 ttttttgggg gggttaagat ttattttatt tatatgggta cactgtagct gtcttctaga 14281 cacaccagaa gagggcatgg gatcccatta cagatggttg tgagccacca tgtggttgct 14341 gggaattgaa ctcaggacct ctggaagagc agtcagtgct cttaaccgct gagccatctc 14401 tccagccctc aaactctttt ttttcttttc cttcaagatg agttctgtgt agtcctggcg 14461 gaccaggttg gcctcagatc agcctgcctc tgcctccgca gtgctgagat taaaggcccg 14521 tgccactcta ggctaaattg ttatgcttct attctagctg atgaccacct tttttgggcg 14581 tagtagtgct gggagtaggg tctgtacaca tgtctacaat gccagaatag gtcaaaggct 14641 ttagatctca aggaactgga tttatagaga gttgggagca gccatgtagg ttctgagaac 14701 caaacctggg tcctctgcaa gaagagccat tggctttttg tttttgtttg ttttgagaca 14761 tttctcggtg tagccctggc tatctggaac tctgtaggcc aggctgtccc tgaactcaga 14821 tccagtctat ccatccctgc cttccaagag ctgggattaa ggtcatgtac caccacaggc 14881 cagctagcca tagctcctaa ctgctgaacc atttatttat ttatttattt tatttttttg 14941 gtttttcgag acagggtttc tctgtatagc tctggatgtc ctggaactca ctttgtaaac 15001 cagtctggcc tcgaactcag aaatctgctt gcctctgcct ccccagtgag tgctgggatc 15061 aaatgcgtgc gccaccactc ccagcttaag tctttatttt ttaaaatgtt atttattttg 15121 gggctggtga gatggctcag tggttaagag cactgactgc tctgccagag gtcccgagtt 15181 caaatcccag caactacatg gtggctcaca accatctgta atgagatctg acgccctctt 15241 ctggtatgtc tgaagacaac tacagtgtac ttacatatat atataataaa taaattaaaa 15301 aaaaaaaaga gaggaggagc caagcagctc ctttagaaaa aaaaaaagtt atttatttta 15361 tgtatttgag ctgtcttcag acacaccaga agagggcaat ggatcccatt acagatggtt 15421 gtgagccacc atggttgatg ggaattgaac tctggacctc tggcagagca gtcagtgctc 15481 ttaaccgctg agccatctct ccagccccca aagccaagtc ttaaagcatt tttgctgctg 15541 aatgtcagcc ctaccagatc tctgcctccc tcccctcccc tccccccagt atctcatgaa 15601 gaccaagctg gcctggacac agtaagtatg tgcatattta tgtgtgaaga ttgtcacaat 15661 gtgaggaaaa aagttggttc cctccatggt gtgggtcctg tgggtcaagt ccaggtcgct 15721 aggcttggca gcaagtgcct tgactcatag tcttctcctg cccaactcca tgcttggttt 15781 ccacgagccc ctgtgctatg gagaattcca tctccaggcc tcaccaaatc taatctcccc 15841 atcctttgaa aagcagactt agattcaacg caagtggatg aaacagttta ttctttattt 15901 gggaataaag actaagctct gaaaagctag tcccagagac tcagctggtg gtgatactag 15961 ctagcggtgg catgaggatg ccttgggaat gtgctctggg tccttcaggg tgctttagcc 16021 gatgccattc aagaacatga gtagggttag ggtattgtgg cagagcactt gcctggtata 16081 tgctggcttc agcaaaataa aaccatacct tctaggaatg gtttctggga ccggtgctct 16141 aactgcaggt atcctggcat ccatggaggc aaggctttat tccttgtgac tgggcttgta 16201 gctcactgga aacttggagg ctgcaacatc tttggcagga aaccatcttt tctgtcactt 16261 catttgcaag cattctccag ccttgagtca gtctttagca atggaccttt ccctgtggtc 16321 attccctttg gagaaagaca ttcctcaaag tccatggtaa ctttgaatga gtgttttgca 16381 tgtacacatg cgtgagtgtg catgcgctct cacacacgca cgcacatgca cacgcgtgca 16441 cacacacaca cacacacaca cacacacaca cacacacaca cactgcttca gcccttagga 16501 gccattcttc tattattatg tttgagtgct ctgcctgaat gtgcacctgc aggccagaag 16561 agggcatcag atccctttta gagatggtca caagccatca tgttgttgct ggaaattggg 16621 acttctggaa gagcagccag tgtaccctta attgctgagc catcttactg cccaagatac 16681 attcttacac tgtgcctgac cctgagccac atctgtgtcc tgactgcaaa gtcaagatgc 16741 cattatggca tcctggatgc tatagccagt gcaggccaga gggtaccaga tgtcacagcc 16801 atcacaccaa cccaggctct gctctctagc aaaaagaagc tggacaggac tccctaaggg 16861 agtgagtgtt cctgagaaac cctttgagta acttgcctct gggtaactgg tagccagaac 16921 aggaggctaa gactgggata taggaacttg gagattaggg atgttaagta gagcatacgc 16981 atagcacaaa agatacttgg ctttggatat gagctgttga cgccttcaat ccatcacaac 17041 tcccattctg aatgctctat cccgactaca tgagggattt gaggctagcc tggattacac 17101 agtgagacct tgtattaaaa aagagttggg tgtctcctcc agagaggatc tgggtttgaa 17161 cctcagcacc tacatagtgg ctagcaatta tccctccagt tcccagagaa cccagtgccc 17221 tcttctggct tctgccggta ttgcatgcaa gtgtgatacc caatcatgca ggcaaacaaa 17281 gcagccttga attgacctgc tctcctctag ttttgagaca gtgttagtat ggttttttat 17341 gtatagtgct gggactaatt aaccctcact aaagggcggc cgcctcgagc cctatagtga 17401 gtcgtattac caaacatggg caagtcaggt gcttgctagg cagtgctctt ctagtgagac 17461 atctctttgt tccctgtctc ccagattgct ttgtatagtc tagtcctaac cattgttccc 17521 acatagtagc ttgtcatgca tttatgggtg aaagctaacc tgggtgtctg ctgtgcccgt 17581 gcacccccct tccctgcctt ctaagacctc agtctgaggc tgttcaaaga tctagaattc 17641 aaggtgctga caggtgaccc cacttaccca ctggctatca gagcagcttt ctccacccgg 17701 agcctcttcc tgggctccgt caggacggac agggggcctg gggagacctc atcatctagc 17761 tctgccaaca ctttatgagc tgatttcctc cggtttcttg tgggtggagc aggcgaaact 17821 tgcgcttccc catgctgaga gggaatagcc aggccctgtg gcctgccacc accacgggag 17881 aaggtgagag gctgggcctg gagtttgctg aggctgccga tggaattgag aatcagtgtg 17941 gccttggggg cagaggagag agtgctgagt attctaccgg gtaggggagg cgcttttccc 18001 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg 18061 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg 18121 tggccccgtc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca 18181 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat 18241 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct 18301 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag 18361 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac 18421 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac 18481 ctgcagcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg 18541 acaaggtgag gaactaaacc atgggatcgg ccattgaaca agatggattg cacgcaggtt 18601 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acgatcggct 18661 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 18721 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 18781 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 18841 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 18901 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 18961 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 19021 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 19081 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 19141 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 19201 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 19261 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gcccccgatt 19321 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 19381 cgaataaaga ccgaccaagc gacgtctgag agctccctgg cgaattcggt accaataaaa 19441 gagctttatt ttcatgatct gtgtgttggt ttttgtgtgc ggcgcgggcc tctgaggtgc 19501 cccttggggg ggcagcatag gttggaaacc gcccccagct tctcccttag accgagggac 19561 ggttatggct gcaaagtcct gaggtttaac ccggacttgt tgaaacatga agtagaactc 19621 tgaggaggag ctggttcctg cttgaccttg ggggccaaag gtcagaaggt caccatcact 19681 caactccagt ctgtggcctc ttggaagtcg gacattattg accaaagtcc ctggtgataa 19741 tgagacagac ataagacagg tgacgagaac aggaacagac ttgtagtcag aattaacatg 19801 atccgaattc gttcccttta gtgagggtta attccgcggc cgctctagaa ctagtggatc 19861 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc 19921 cggtacccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt 19981 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 20041 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 20101 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 20161 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 20221 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 20281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 20341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 20401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 20461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 20521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 20581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 20641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 20701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 20761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 20821 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 20881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 20941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 21001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 21061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 21121 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 21181 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 21241 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 21301 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 21361 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 21421 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 21481 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 21541 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 21601 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 21661 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 21721 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 21781 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 21841 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 21901 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 21961 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 22021 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 22081 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac //