LOCUS Exported 6505 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLIA (CC#1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6505) AUTHORS Bao ZZ, Cepko CL TITLE The expression and function of Notch pathway genes in the developing rat eye. JOURNAL J Neurosci. 1997 Feb 15. 17(4):1425-34. PUBMED 9006984 REFERENCE 2 (bases 1 to 6505) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6505 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(66..83) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(237..825) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(317..336) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1008..1868) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1631..1650 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1869..1973) /gene="bla" /label=AmpR promoter misc_feature 2539..2738 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 2939..3355 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 3285..3307 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 3323..3339 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 3417..3983 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 3434..3985 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3578..3595) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3805..3824 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" LTR 5911..6504 /note="long terminal repeat from Moloney murine leukemia virus" ORIGIN 1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttgcggcc 961 ggccgcaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 1021 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 1081 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 1141 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 1201 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 1261 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 1321 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 1381 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 1441 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 1501 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 1561 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 1621 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 1681 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 1741 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 1801 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 1861 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 1921 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 1981 ccccgaaaag tgccacctga cgtgctgcag aatatgggcc aaacaggata tctgtggtaa 2041 gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca 2101 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga 2161 tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg tgcccccaag 2221 aacctgaaat gaccctggcc ttatttgaac taaccaatca gttcgtttct cgtttctgtt 2281 ggggcgcttc tgttcccgga gctcaataaa agagcccaca acccttcatt gggggcgcca 2341 gtcttccgat tgactgagtc gcccggtacc gtgtatccaa taaaccttct tgcagttgca 2401 tcagacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg acaacccgtc 2461 agcggggtct ttcatttggg ggctcgtccg ggatcgggag acccttgccc agggaccacg 2521 gacccaccac cgggaggcaa gctggccagc aacttatctg tgtctgtccg attgtctagt 2581 gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc tctgtatctg 2641 gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc 2701 cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc 2761 gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc 2821 gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg 2881 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg 2941 ccagactgtt accaatccct taagtttgac cttaggtcac tggaaagatg tcgagcggat 3001 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga 3061 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac 3121 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta 3181 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca 3241 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg 3301 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggccg 3361 gatccgcggc cgcataggcc actagtggat cccagtgtgg tggtacgtag gaattctccc 3421 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc 3481 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc 3541 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt 3601 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct 3661 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa 3721 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt 3781 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag 3841 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt 3901 acgtgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt 3961 tcctttgaaa aacacgatga taatatggcc aaatatgggg tcgaggctgc tgctgctgct 4021 gctgctgggc ctgaggctac agctctccct gggcatcatc ctagttgagg aggagaaccc 4081 ggacttctgg aaccgcgagg cagccgaggc cctgggtgcc gccaagaagc tgcagcctgc 4141 acagacagcc gccaagaacc tcatcatctt cctgggcgat gggatggggg tgtctacggt 4201 gacagctgcc aggatcctaa aagggcagaa gaaggacaaa ctggggcctg agatacccct 4261 ggccatggac cgcttcccat atgtggctct gtccaagaca tacaatgtag acaaacatgt 4321 gccagacagt ggagccacag ccacggccta cctgtgcggg gtcaagggca acttccagac 4381 cattggcttg agtgcagccg cccgctttaa ccagtgcaac acgacacgcg gcaacgaggt 4441 catctccgtg atgaatcggg ccaagaaagc agggaagtca gtgggagtgg taaccaccac 4501 acgagtgcag cacgcctcgc cagccggcac ctacgcccac acggtgaacc gcaactggta 4561 ctcggacgcc gacgtgcctg cctcggcccg ccaggagggg tgccaggaca tcgctacgca 4621 gctcatctcc aacatggaca ttgatgtgat cctaggtgga ggccgaaagt acatgtttcg 4681 catgggaacc ccagaccctg agtacccaga tgactacagc caaggtggga ccaggctgga 4741 cgggaagaat ctggtgcagg aatggctcgg cgaacgccag ggtgcccggt acgtgtggaa 4801 ccgcactgag ctcatgcagg cttccctgga cccgtctgtg acccatctca tgggtctctt 4861 tgagcctgga gacatgaaat acgagatcca ccgagactcc acactggacc cctccctgat 4921 ggagatgaca gaggctgccc tgcgcctgct gagcagacac ccccgcggct tcttcctctt 4981 cgtggagggt ggtcgcatcg accatggtca tcatgaaagc agggcttacc gggcactgac 5041 tgagacgatc atgttcgacg acgccattga gagggcgggc cagctcacca gcgaggagga 5101 cacgctgagc ctcgtcactg ccgaccactc ccacgtcttc tccttcggag gctaccccct 5161 gcgagggagc tccttcatcg ggctggccgc tggcaaggcc cgggacagga aggcctacac 5221 ggtcctccta tacggaaacg gtccaggcta tgtgctcaag gacggcgccc ggccggatgt 5281 taccgagagc gagagcggga gccccgagta tcggcagcag tcagcagtgc ccctggacga 5341 agagacccac gcaggcgagg acgtggcggt gttcgcgcgc ggcccgcagg cgcacctggt 5401 tcacggcgtg caggagcaga ccttcatagc gcacgtcatg gccttcgccg cctgcctgga 5461 gccctacacc gcctgcgacc tggcgccccc cgccggcacc accgacgccg cgcacccggg 5521 gcggtccgtg gtccccgcgt tgcttcctct gctggccggg accctgctgc tgctggagac 5581 ggccactgct ccctgagtgt cccgtccctg gggctcctgc ttccccatcc cggagttctc 5641 ctgctcccca cctcctgtcg tcctgcctgg cctccagccc gagtcgtcat ccccggagtc 5701 cctatacaga ggtcctgcca tggaaccttc ccctccccgt gcgctctggg gactgagccc 5761 atgacaccaa acctgcccct tggctgctct cggactccct accccaaccc cagggactgc 5821 aggttgtgcc ctgtggctgc ctgcacccca ggaaaggagg gggctcaggc catccagcca 5881 ccacctacag cccagtgggt acggtcgacc aatgaaagac cccacctgta ggtttggcaa 5941 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa 6001 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt 6061 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc 6121 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc 6181 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc 6241 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt 6301 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg 6361 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc 6421 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact 6481 acccgtcagc gggggtcttt cattt //