LOCUS Exported 4676 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSH1/Mf-del-Akt-E SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4676) AUTHORS Park D, Lapteva N, Seethammagari M, Slawin KM, Spencer DM TITLE An essential role for Akt1 in dendritic cell function and tumor immunotherapy. JOURNAL Nat Biotechnol. 2006 Dec . 24(12):1581-90. PUBMED 17143278 REFERENCE 2 (bases 1 to 4676) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4676 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(66..83) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(237..825) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(317..336) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(996..1856) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1619..1638 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1857..1961) /gene="bla" /label=AmpR promoter primer_bind 2029..2047 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(2109..2129) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2114..2729 /label=SR-alpha promoter /note="hybrid promoter consisting of the SV40 early promoter plus part of the long terminal repeat of human T-lymphotrophic virus 1" rep_origin 2294..2429 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2356..2375 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" LTR 2460..2729 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" intron 2774..2867 /label=SV40 intron /note="modified SV40 intron with splice donor and acceptor sites" regulatory 2938..2947 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4147..4173 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" polyA_signal 4204..4338 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4228..4247) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 4282..4301 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(4478..4500) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 4641..4660 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 1201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 1261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 1321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 1381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 1441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 1501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 1561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 1621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 1681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 1741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 1801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 1861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 1921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 1981 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 2041 acgaggccct ttcgtcttca aggtcgatcg acctcatgtt tgacagctta tcatcgataa 2101 gcttctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 2161 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 2221 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 2281 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 2341 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 2401 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc tccgatcgag 2461 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccaacgccgg 2521 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 2581 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 2641 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 2701 tcgttttctg ttctgcgccg ttacagatcc aagctgcctc gatcgaggaa ctgaaaaacc 2761 agaaagttaa ctggtaagtt tagtcttttt gtcttttatt tcaggtcccg gatccggtgg 2821 tgcaaatcaa agaactgctc ctcagtggat gttgccttta cttctaggcc tgtacggagg 2881 tgttacttct gctctaaaag ctgctctagc cctcgatcga gggctagagc ggccgcggcc 2941 accatgggct gtgtgcaatg taaggataaa gaagcaacaa aactgacgga ggagctcgac 3001 accgccattc agactgtggc cgatggactc aagaggcagg aagaagagac gatggacttc 3061 cgatcaggct cacccagtga caactcaggg gctgaagaga tggaggtgtc cctggccaag 3121 cccaagcacc gtgtgaccat gaacgagttt gagtacctga aactactggg caagggcacc 3181 tttgggaaag tgattctggt gaaagagaag gccacaggcc gctactatgc catgaagatc 3241 ctcaagaagg aggtcatcgt cgccaaggat gaggttgccc acacgcttac tgagaaccgt 3301 gtcctgcaga actctaggca tcccttcctt acggccctca agtactcatt ccagacccac 3361 gaccgcctct gctttgtcat ggagtatgcc aacgggggcg agctcttctt ccacctgtct 3421 cgagagcgcg tgttctccga ggaccgggcc cgcttctatg gtgcggagat tgtgtctgcc 3481 ctggactact tgcactccga gaagaacgtg gtgtaccggg acctgaagct ggagaacctc 3541 atgctggaca aggacgggca catcaagata acggacttcg ggctgtgcaa ggaggggatc 3601 aaggatggtg ccactatgaa gacattctgc ggaacgccgg agtacctggc ccctgaggtg 3661 ctggaggaca acgactacgg ccgtgcagtg gactggtggg ggctgggcgt ggtcatgtat 3721 gagatgatgt gtggccgcct gcccttctac aaccaggacc acgagaagct gttcgagctg 3781 atcctcatgg aggagatccg cttcccgcgc acactcggcc ctgaggccaa gtccctgctc 3841 tccgggctgc tcaagaagga ccctacacag aggctcggtg ggggctctga ggatgccaag 3901 gagatcatgc agcaccggtt ctttgccaac atcgtgtggc aggatgtgta tgagaagaag 3961 ctgagcccac ctttcaagcc ccaggtcacc tctgagactg acaccaggta tttcgatgag 4021 gagttcacag ctcagatgat caccatcacg ccgcctgatc aagatgacag catggagtgt 4081 gtggacagtg agcggaggcc gcacttcccc cagttctcct actcagccag tggcacagcc 4141 gtcgactatc cgtacgacgt accagactac gcactcgact aagaattcct gattgattga 4201 ccggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag 4261 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata 4321 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg 4381 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat 4441 gatcctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 4501 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 4561 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag 4621 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accata //