LOCUS Exported 830 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Expresses truncated version of NLL-MetRS from endogenous MetG promoter. ACCESSION . VERSION . KEYWORDS pAM1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 830) AUTHORS Mahdavi A, Szychowski J, Ngo JT, Sweredoski MJ, Graham RL, Hess S, Schneewind O, Mazmanian SK, Tirrell DA TITLE Identification of secreted bacterial proteins by noncanonical amino acid tagging. JOURNAL Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):433-8. doi: 10.1073/pnas.1301740111. Epub 2013 Dec 17. PUBMED 24347637 REFERENCE 2 (bases 1 to 830) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..830 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 8..25 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" terminator 89..183 /label=lambda t0 terminator /note="transcription terminator from phage lambda" ORIGIN 1 aggatcgcat caccatcacc atcacggatc cgcatgcgag ctcggtaccc cgggtcgacc 61 tgcagccaag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca 121 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag 181 aatccaagct agctctagag acgtccggcc ggagctccac cgcggtggcg gccgctctag 241 agtcacttac ttaacatttt cccatttggt actatctaac cccttttcac tattaagaag 301 taatgcctac tatgactcaa gtcgcgaaga aaattctggt gacgtgcgca aacccgtacg 361 ctaacggctc aatccacctc ggccatatgc tggagcacat ccaggctgat gtctgggtcc 421 gttaccagcg aatgcgcggc cacgaggtca acttcatctg cgccgacgat gcccacggta 481 caccgatcat gctgaaagct cagcagcttg gtatcacccc ggagcagatg attggcgaaa 541 tgagtcagga gcatcagact gatttcgcag gctttaacat cagctatgac aactatcact 601 cgacgcacag cgaagagaac cgccagttgt cagaacttat ctactctcgc ctgaaagaaa 661 acggttttat taaaaaccgc accatctctc agctgtacga tccggaaaaa ggcatgttcc 721 tgccggaccg ttttgtgaaa ggcacctgcc cgaaatgtaa atccccggat caatacggcg 781 ataactgcga agtctgcggc gcgacctaca gcccgactga actgatcgag //