LOCUS Exported 840 bp ds-DNA linear SYN 29-MAY-2021 DEFINITION Cre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophore . ACCESSION . VERSION . KEYWORDS AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 840) TITLE ChIEF and oChIEF AAV vectors JOURNAL Unpublished REFERENCE 2 (bases 1 to 840) AUTHORS . TITLE Direct Submission JOURNAL Exported May 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..840 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 33..66 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." protein_bind 117..150 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." primer_bind complement(251..274) /label=DsRed1-C /note="DsRed1, forward primer" primer_bind complement(449..468) /label=mCherry-F /note="mCherry, forward primer" primer_bind 718..736 /label=mCherry-R /note="mCherry, reverse primer" ORIGIN 1 gaggtaccgg atcctctaga gtcgactccg gaataacttc gtataggata ctttatacga 61 agttatgcag aatggtagct ggattgtagc tgctattagc aatatgaaac ctcttaataa 121 cttcgtatag catacattat acgaagttat ggcgcgccgt cgacttactt gtacagctcg 181 tccatgccgt acaggaacag gtggtggcgg ccctcggagc gctcgtactg ttccacgatg 241 gtgtagtcct cgttgtggga ggtgatgtcc agcttggtgt ccacgtagta gtagccgggc 301 agttgcacgg gcttcttggc catgtagatg gtcttgaact ccaccaggta gtggccgccg 361 tccttcagct tcagggcctg gtggatctcg cccttcagca cgccgtcgcg ggggtacagg 421 cgctcggtgg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggggggg 481 aagttggtgc cgcgcatctt caccttgtag atcagcgtgc cgtcctgcag ggaggagtcc 541 tgggtcacgg tcaccagacc gccgtcctcg aagttcatca cgcgctccca cttgaagccc 601 tcggggaagg acagcttctt gtaatcgggg atgtcggcgg ggtgcttcac gtacgccttg 661 gagccgtaca tgaactgggg ggacaggatg tcccaggcga agggcagggg gccgcccttg 721 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg 781 atctcgaact cgtggccgtt catggagccc tccatgcgca ccttgaagcg catgaactct //