LOCUS       Exported                6624 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCL25
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Luong TT, Lee CY
  TITLE     Improved single-copy integration vectors for Staphylococcus aureus.
  JOURNAL   J Microbiol Methods. 2007 Jul . 70(1):186-90.
  PUBMED    17512993
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(2880..3671)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(4923..5873)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5921..6143
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttttt
       61 tattacctac aacttcttta gcacttaaac gaccatcatc agttattgga acaacgccat
      121 aatgcatatg tggtgttttt tcgtccatgt gaactgttgc atataataaa ttatctttac
      181 cgtattcttg ttctaaaaac tctttagcat attcaaaaaa ctgctttgta tcttctggcg
      241 tttgattatc aaagaaatca ttgtctgatg taattaaacc atcaatgtgt ttaatcgcgt
      301 ctgttctaat ttttcttttg cctgtataat tctgttcgat tttttcatca atcaagttat
      361 taaaattctg tttattagca tttaccaaat cataatttaa gtaagtttta ctatggtcta
      421 tatcttcatt ttcataatta ttattttctc tttgaacatg tttttgtatg cccgttgtat
      481 ttgttccaga tttaactttt gaaactctaa caatggaata agacatataa aaatcactcc
      541 taagcacaaa ggttttatgt gtatttaata gtttaagtct aacacactag acttattttt
      601 aataagtcgt taatacgtgt gctctgcgag gcttaaacct gtttttgcta acgcaaaaaa
      661 tgagtggcaa aatgctagcc actcatagtt ctaaaccaaa atataatata actattcaaa
      721 ctgcttttca gaacgtttaa atacaataat cgtcaaaaga caacataaaa taataaggat
      781 agccatggct acaagaatat tactatacac tccagaagaa taatttataa attccagaac
      841 tagtttacga ttaatcaatt gtagtgacaa taaacctcct acaattgcta tacctgttcc
      901 ctctgataaa aaacttgtga aatttagcaa actcattcca gaagcaactt cttcttcaga
      961 aagactactt gatactattt ttgatataac tgttttagta aaagataatc cgcccataac
     1021 aaatataaac ataaaagtag tcaaccacat actaaactca acaaaaaatg caatagttaa
     1081 aaaacttata gagatagaca atgatcctaa aataaaaaca aataatgatc cttttctatc
     1141 cactaaaaaa ccaccaaaat aaccaaaaac aataacactc atggttccag gaaaaataac
     1201 actattacct attgtcgcta catttacatg ataaatagtt ttcatcatat aaggcaccat
     1261 tgatataaaa ccagctacta tagaaaatat tagcccacca gaaaacaaac caagcataaa
     1321 cggaatgttt ttccctagtt taggattaat aaaagggtta gaaactcttg aaatatgttt
     1381 aataaaaatc acaaaaaaga ttgtgaagag tattaaaaaa gtccaattat aatttgtcgt
     1441 aaataacata aaacatataa tacttataga cattaaaaca atacctacga tatctaatgt
     1501 attttttgtt gatttaccag gtaccattac tttaataaga aaaggtatag ttactattgt
     1561 aatcatagga agtataagta ggtaagacca atgaatataa tgtgctatta ttccccctat
     1621 tgaaggacct aacccttcac ctaaagctac aattgatcct ataaaaccaa aggctttgcc
     1681 ttgttttttt cttgtaatat ttctagctac aaccaccata atcagtgaag ggaatgcagc
     1741 agatcctact ccttgtacta acctaccaaa aatcaaaata aaaaagtgat tgtgaccaat
     1801 aaaagcaatc aatgaaccaa gacagctcaa actaatacca ataattaaca atttttttat
     1861 atttatataa tcagataatt ttccatatac tgctgttcct atcgaaaaag ttaacatata
     1921 tgcagtgttt acccagtttg taattccagg agtagtatta aaatgatttg caatatcagg
     1981 taaagaaaca tttaaaacca tttcatttaa tacactaaaa aatgaaagaa tacaaagcca
     2041 aaataaaacg ctataaaaca aacctttaaa ttttttatat aaactaaaca attttacctc
     2101 tttacttgag gtgactaaag tttatagggg taaattattc tttcacctca tataaattcc
     2161 ccaaattttt aaatagacac ttcataaaaa aatcctccta aattagaata gtttaaatat
     2221 atcatttttg ttcagtaata ttaatatgta cactatttcc aaaatttaaa ttcatgttgc
     2281 caaaaatcga ttatgtcttt tgcggggcaa aaaaagggca gattatttga aaaagggcaa
     2341 acgcttgtgg aaaagctaaa aggttaaaaa tgacaaaaac cttgatacaa cagtgttttt
     2401 ggacgctcgt gtacgttaga gaatgaccgg tttaccatca tacaaggatg ggattaactt
     2461 gtgttaaaaa gcctttaata tcagttgtta caaaggattt gtagcgtctt taaaaataaa
     2521 aaagggcaga aaaagggcag atacctttta gtacacaagt ttttctaatt tttgctctaa
     2581 ctctctgtcc attttctctg ttacatgtgt atacaccttt atagtcgttt tttccagatc
     2641 tggatctgga ttttaatgcg gatgttgcga ttacttcgcc aactattgcg ataacaagaa
     2701 aaagccagcc tttcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa
     2761 aataataaaa gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat
     2821 ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat tgttagacat
     2881 tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga
     2941 tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt
     3001 atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc
     3061 ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt
     3121 cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc
     3181 tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag
     3241 gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct
     3301 ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc
     3361 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg
     3421 cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct
     3481 cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt
     3541 ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg
     3601 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc
     3661 gcttccctca tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     3721 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
     3781 ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     3841 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
     3901 cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc
     3961 cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg
     4021 ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc ggccttgctg
     4081 ttcttctacg gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct
     4141 cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt
     4201 tttctggaag gcgagcatcg tttgttcgcc cagcttctgt atggaacggg catgcggatc
     4261 agtgagggtt tgcaactgcg ggtcaaggat ctggatttcg atcacggcac gatcatcgtg
     4321 cgggagggca agggctccaa ggatcgggcc ttgatgttac ccgagagctt ggcacccagc
     4381 ctgcgcgagc aggggaatta attcccacgg gttttgctgc ccgcaaacgg gctgttctgg
     4441 tgttgctagt ttgttatcag aatcgcagat ccggcttcag ccggtttgcc ggctgaaagc
     4501 gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact ggctcccgtg
     4561 ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct atgtgtgact
     4621 gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc tttgttttac
     4681 tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg tcgatctgtt
     4741 catggtgaac agctttgaat gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt
     4801 ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatgtaa cggtgaacag
     4861 ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa
     4921 cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg
     4981 agccatgaga acgaaccatt gagatcatac ttactttgca tgtcactcaa aaattttgcc
     5041 tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg
     5101 ttatgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc
     5161 tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc
     5221 aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt
     5281 aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta
     5341 ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga
     5401 gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt
     5461 tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa
     5521 ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt
     5581 gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc
     5641 atcagctctc tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc
     5701 atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca
     5761 atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag
     5821 tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc
     5881 aattggtcta ggtgatttta atcactatac caattgagat gggctagtca atgataatta
     5941 ctagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt tgctggaaaa
     6001 cttgtaaatt ctgctagacc ctctgtaaat tccggtagac ctttgtgtgt tttttttgtt
     6061 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
     6121 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
     6181 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
     6241 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     6301 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     6361 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     6421 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     6481 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     6541 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     6601 caggcttacc cgtcttactg tcgg
//
