LOCUS Exported 908 bp ds-DNA linear SYN 07-JUN-2021 DEFINITION Cre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophore . ACCESSION . VERSION . KEYWORDS AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 908) TITLE ChIEF and oChIEF AAV vectors JOURNAL Unpublished REFERENCE 2 (bases 1 to 908) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 7, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..908 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 47..80 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind 131..164 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." regulatory 174..183 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcataa cttcgtatag 61 catacattat acgaagttat tctttgcacc attctaaaga ataacagtga taatttctgg 121 gttaaggcaa ataacttcgt ataggatact ttatacgaag ttatgctagc ggcgccacca 181 tggtgagcag aagaccctgg ctgctggccc tggccctggc cgtggccctg gccgccggca 241 gcgccggcgc cagcaccggc agcgacgcca ccgtgcccgt ggccacccag gacggccccg 301 actacgtgtt ccacagagcc cacgagagaa tgctgttcca gaccagctac accctggaga 361 acaacggcag cgtgatctgc atccccaaca acggccagtg cttctgcctg gcctggctga 421 agagtaacgg caccaacgcc gagaagctgg ccgccaacat cctgcagtgg atcaccttcg 481 ccctgagcgc cctgtgcctg atgttctacg gctaccagac ctggaagagt acctgcggct 541 gggaggagat ctacgtggcc accatcgaga tgatcaagtt catcatagag tacttccacg 601 agttcgacga gcccgccgtg atctacagca gcaacggcaa caagaccgtg tggctgagat 661 acgccgcctg gctgctgacc tgccccgtgg tcctgatcca cctgagcaac ctgaccggcc 721 tggccaacga ctacaacaag agaaccatgg gcctgctggt gagcgacatc ggctgtatcg 781 tgtggggcac caccgccgcc ctgagcaagg gctacgtgag agtgatcttc ttcctgatgg 841 gcctgtgcta cggcatctac accttcttca acgccgccaa ggtgtacatc gaggcctacc 901 acaccgtg //