LOCUS       Exported                 815 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  The plasmid contains a Pleaides (Ple) MiniPromoter derived from the 
            human PCP2 gene and designed for expression in ON Bipolar cells of 
            the retina..
ACCESSION   .
VERSION     .
KEYWORDS    pEMS1986
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Korecki AJ, Cueva-Vargas JL, Fornes O, Agostinone J, Farkas RA, 
            Hickmott JW, Lam SL, Mathelier A, Zhou M, Wasserman WW, Di Polo A, 
            Simpson EM
  TITLE     Human MiniPromoters for ocular-rAAV expression in ON bipolar, cone, 
            corneal, endothelial, Muller glial, and PAX6 cells.
  JOURNAL   Gene Ther. 2021 Feb 2. pii: 10.1038/s41434-021-00227-z. doi: 
            10.1038/s41434-021-00227-z.
  PUBMED    33531684
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             100..111
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     509..529
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg
       61 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta
      121 gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga
      181 cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca
      241 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa
      301 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc
      361 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga
      421 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg
      481 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat
      541 accagtcaat ctttcacaaa ttttgtaatc cagaggttga gcgatcgcgg ccgctcagtc
      601 cccatcctcg agcagcctca ccatggcccc agtctcagag tccaggtttc tgatgtagtt
      661 catcactatg ttcacattgg tccagccacc agcctgcatg atttcaggga tggacacacc
      721 agccctggcc atgtccctgg cagcacccac tctggcagag tggccagacc aggccaggta
      781 tctctgccca gagtcatcct tggcaccata gatca
//
