LOCUS Exported 9907 bp ds-DNA circular SYN 30-NOV-2022 DEFINITION transcribes pactamycin resistant 18S. ACCESSION . VERSION . KEYWORDS p18S.1 G693A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9907) AUTHORS Burman LG, Mauro VP TITLE Analysis of rRNA processing and translation in mammalian cells using a synthetic 18S rRNA expression system. JOURNAL Nucleic Acids Res. 2012 Sep;40(16):8085-98. doi: 10.1093/nar/gks530. Epub 2012 Jun 20. PUBMED 22718970 REFERENCE 2 (bases 1 to 9907) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 30, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9907 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 8115..8219 /gene="bla" /label=AmpR promoter CDS 8220..9080 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8438..8457) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9251..9839 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9740..9759 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gatctgggtc gaccagttgt tcctttgagg tccggttctt ttcgttatgg ggtcattttt 61 gggccacctc cccaggtatg acttccaggt attctctgtg gcctgtcact ttcctccctg 121 tctcttttat gcttgtgatc ttttctatct gttcctattg gacctggaga taggtactga 181 cacgctgtcc tttccctatt aacactaaag gacactataa agagaccctt tcgatttaag 241 gctgttttgc ttgtccagcc tattcttttt actggcttgg gtctgtcgcg gtgcctgaag 301 ctgtccccga gccacgcttc ctgctttccc gggcttgctg cttgcgtgtg cttgctgtgg 361 gcagcttgtg acaactgggc gctgtgactt tgctgcgtgt cagacgtttt tcccgatttc 421 cccgaggtgt cgttgtcaca cctgtcccgg ttggaatggt ggagccagct gtggttgagg 481 gccaccttat ttcggctcac tttttttttt tttttttctc ttggagtccc gaacctccgc 541 tcttttctct tcccggtctt tcttccacat gcctcccgag tgcatttctt tttgtttttt 601 ttcttttttt tttttttttt tttggggagg tggagagtcc cgagtacttc actcctgtct 661 gtggtgtcca agtgttcatg ccacgtgcct cccgagtgca cttttttttg tggcagtcgc 721 tcgttgtgtt ctcttgttct gtgtctgccc gtatcagtaa ctgtcttgcc ccgcgtgtaa 781 gacattccta tctcgcttgt ttctcccgat tgcgcgtcgt tgctcactct tagatcgatg 841 tggtgctccg gagttctctt cgggccaggg cctaagccgc gccaggcgag ggacggacat 901 tcatggcgaa tggcggccgt tcttctcgtt ctgccagcgg gcccctcgtc tctccacccc 961 atccgtctgc cggtggtgtg tggaaggcag gggtgcggct ctccggcccg agcgctgccc 1021 cgcgcgcact tttctcagtg gttcgcgtgg tccttgtgga tgtgtgaggc gcccggttgt 1081 gccctcacgt gtttcacttt ggtcgtgtct cgcttgacca tgttcccaga gtcggtggat 1141 gtggccggtg gcgttgcata cccttcccgt ctggtgtgtg cacgcgctgt ttcttgtaag 1201 cgtcgaggtg ctcctggagc gttccaggtt tgtctcctag gtgcctgctt ctgagctggt 1261 ggtggcgctc cccattccct ggtgtgcctc cggtgctccg tctggctgtg tgccttcccg 1321 tttgtgtctg agaagcccgt gagagggggg tcgaggagag aaggaggggc aagacccccc 1381 ttcttcgtcg ggtgagcgcc caccccgcgc actagtacgc ctgtgcgtag ggctggtgct 1441 gagcggtcgc ggctggcctt ggaaagtttc tcgagagact cattgctttc ccgtggggag 1501 ctttgagagg cctggctttc gggggggacc ggttgcaggg tctcccctgt ccgcggatgc 1561 tcagaatgcc cttggaagag aaccttcctg ttgccgcaga cccccccgcg cggtcgcccg 1621 cgtgttggtc ttctggtttc cctgtgtgct cgtcgcatgc atcctctctc ggtggccggg 1681 gctcgtcggg gttttgggtc cgtcccgccc tcagtgagaa agtttccttc tctagctatc 1741 ttccggaaag ggtgcgggct tcttacggtc tcgaggggtc tctcccgaat ggtcccctgg 1801 agggctcgcc ccctgaccgc ctcccgcgcg cgcagcgttt gctctctcgt ctaccgcggc 1861 ccgcgcctcc ccgctccgag ttcggggagg gatcacgcgg ggcagagcct gtctgtcgtc 1921 ctgccgttgc tgcggagcca tgtggctccg gttgtgtggt tggtggctgg ggagagggct 1981 ccgtgcacac ccccgcgtgc gcggtacttt cctcccctcc tgagggccgc cgtgcgggac 2041 ggggtgtggg taggcgacgg tgggctcccg ggtccccacc cgtcttcccg tgcctcaccc 2101 gtgccttccg tcgcgtgcgt ccctctcgct cgcgtccacg actttggccg ctcccgcgac 2161 gcgggcctgc gccgcgcgtg gtgcgtgctg tgtgcttctc gggctgtgtg gttgtgtcgc 2221 ctcgcccccc ccttcccgcg gcagcgttcc cacggctggc gaaatcgcgg gagtcctcct 2281 tcccctcctc ggggtcgaga gggtccgtgt ctggcgttga ttgatctcgc tctcggggac 2341 gggaccgttc tgtgggagaa cggctgttgg ccgcgtccgg cgcgacgtcg gacgtgggga 2401 cccactgccg ctcgggggtc ttcgtcggta ggcatcggtg tgtcggcatc ggtctctctc 2461 tcgtgtcggt gtcgcctcct cgggctcccg gggggccgtc gtgtttcggg tcggctcggc 2521 gctgcaggtg tggtgggact gctcagggga gtggtgcagt gtgattcccg ccggttttgc 2581 ctcgcgtgcc ctgaccggtc cgacgcccga gcggtctctc ggtcccttgt gaggaccccc 2641 ttccgggagg ggcccgtttc ggccgccctt gccgtcgtcg ccggccctcg ttctgctgtg 2701 tcgttccccc ctccccgctc gccgcagccg gtcttttttc ctctctcccc ccctctcctc 2761 tgactgaccc gtggccgtgc tgtcggaccc cccgcatggg gggcggccgg gcacgtacgc 2821 gtccgggcgg tcaccggggt cttggggggg ggccgagggg taagaaagtc ggctcggcgg 2881 gcgggaggag ctgtggtttg gagggcgtcc cggccccgcg gccgtggcgg tgtcttgcgc 2941 ggtcttggag agggctgcgt gcgaggggaa aaggttgccc cgcgagggca aagggaaaga 3001 ggctagcagt ggtcattgtc ccgacggtgt ggtggtctgt tggccgaggt gcgtctgggg 3061 ggctcgtccg gccctgtcgt ccgtcgggaa ggcgcgtgtt ggggcctgcc ggagtgccga 3121 ggtgggtacc ctggcggtgg gattaacccc gcgcgcgtgt cccggtgtgg cggtgggggc 3181 tccggtcgat gtctacctcc ctctccccga ggtctcaggc cttctccgcg cgggctctcg 3241 gccctcccct cgttcctccc tctcgcgggg ttcaagtcgc tcgtcgacct cccctcctcc 3301 gtccttccat ctctcgcgca atggcgccgc ccgagttcac ggtgggttcg tcctccgcct 3361 ccgcttctcg ccgggggctg gccgctgtcc ggtctctcct gcccgacccc cgttggcgtg 3421 gtcttctctc gccggcttcg cggactcctg gcttcgcccg gagggtcagg gggcttcccg 3481 gttccccgac gttgcgcctc gctgctgtgt gcttgggggg ggcccgctgc ggcctccgcc 3541 cgcccgtgag cccctgccgc acccgccggt gtgcggtttc gcgccgcggt cagttgggcc 3601 ctggcgttgt gtcgcgtcgg gagcgtgtcc gcctcgcggc ggctagacgc gggtgtcgcc 3661 gggctccgac gggtggccta tccagggctc gcccccgccg tcccccgcct gcccgtcccg 3721 gtggtggtcg ttggtgtggg gagtgaatgg tgctaccggt cattccctcc cgcgtggttt 3781 gactgtctcg ccggtgtcgc gcttctcttt ccgccaaccc ccacgccaac ccaccaccct 3841 gtgctccgcg cccggtgcgg tcgacgttcc ggctctcccg atgccgaggg gttcgggatt 3901 tgtgccgggg acggagggga gagcgggtaa gagaggtgtc ggagagctgt cccggggcga 3961 cgctcgggtt ggctttgccg cgtgcgtgtg ctcgcggacg ggttttgtcg gaccccgacg 4021 gggtcggtcc ggccgcatgc actctcccgt tccgcgcgag cgcccgcccg gctcaccccc 4081 ggtttgtcct cccgcgaggc tctccgccgc cgccgcctcc tcctcctctc tcgcgctctc 4141 tgtcccgcct ggtcctgtcc cacccccgac ggcttcgctc gcgcttcctt acctggttga 4201 tcctgccagt agcatatgct tgtctcaaag attaagccat gcatgtctaa gtacgcacgg 4261 ccggtacagt gaaactgcga atggctcatt aaatcagtta tggttccttt ggtcgctcgc 4321 tcctctccta cttggataac tgtggtaatt ctagagctaa tacatgccga cgggcgctga 4381 ccccccttcc cgggggggga tgcgtgcatt tatcagatca aaaccaaccc ggtgagctcc 4441 ctcccggctc cggccggggg tcgggcgccg gcggctttgg tgactctaga taacctcggg 4501 ccgatcgcac gccccccgtg gcggcgacga cccattcgaa cgtctgccct atcaactttc 4561 gatggtagtc gccgtgccta ccatggtgac cacgggtgac ggggaatcag ggttcgattc 4621 cggagaggga gcctgagaaa cggctaccac atccaaggaa ggcagcaggc gcgcaaatta 4681 cccactcccg acccggggag gtagtgacga aaaataacaa tacaggactc tttcgaggcc 4741 ctgtaattgg aatgagtcca ctttaaatcc tttaacgagg atccattgga gggcaagtct 4801 ggtgccagca gccgcggtaa ttccagctcc aatagcgtat attaaagttg ctgcagttaa 4861 aaagctcgta gttggatctt gggagcgggc gggcggtccg ccgcgaggcg agtcaccgcc 4921 cgtccccgcc ccttgcctct cggcgccccc tcgatgctct tagctgagtg tcccgcgggg 4981 cccgaagcgt ttactttgaa aaaattagag tgttcaaagc aggcccgagc cgcctggata 5041 ccgcagctag gaataatgga ataggaccgc ggttctattt tgttggtttt cggaactgag 5101 gccatgatta agagggacgg ccgggggcat tcgtattgcg ccgctagagg tgaaattctt 5161 gaaccggcgc aagacggacc agagcgaaag catttgccaa gaatgttttc attaatcaag 5221 aacgaaagtc ggaggttcga agacgatcag ataccgtcgt agttccgacc ataaacgatg 5281 ccgactggcg atgcggcggc gttattccca tgacccgccg ggcagcttcc gggaaaccaa 5341 agtctttggg ttccgggggg agtatggttg caaagctgaa acttaaagga attgacggaa 5401 gggcaccacc aggagtggag cctgcggctt aatttgactc aacacgggaa acctcacccg 5461 gcccggacac ggacaggatt gacagattga tagctctttc tcgattccgt gggtggtggt 5521 gcatggccgt tcttagttgg tggagcgatt tgtctggtta attccgataa cgaacgagac 5581 tctggcatgc taactagtta cgcgaccccc gagcggtcgg cgtcccccaa cttcttagag 5641 ggacaagtgg cgttcagcca cccgagattg agcaataaca ggtctgtgat gcccttagat 5701 gtccggggct gcacgcgcgc tacactgact ggctcagcgt gtgcctaccc tacgccggca 5761 ggcgcgggta acccgttgaa ccccattcgt gatggggatc ggggattgca attattcccc 5821 atgaacgagg aattcccagt aagtgcgggt cataagcttg cgttgattaa gtccctgccc 5881 tttgtacaca ccgcccgtcg ctactaccga ttggatggtt tagtgaggcc ctcggatcgg 5941 ccccgccggg gtcggcccac ggccctggcg gagcgctgag aagacggtcg aacttgacta 6001 tctagaggaa gtaaaagtcg taacaaggtt tccgtaggtg aacctgcgga aggatcatta 6061 acgggagact gtggaggagc ggcggcgtgg ctcgctctcc ccgtcttgtg tgtgtcctcg 6121 ccgggaggcg cgtgcgtccc gggtcccgtc gcccgcgtgt ggagcgaggt gtctggagtg 6181 aggtgagaga aggggtgggg tggggtcggt ctgggtccgt ctgggaccgc ctccgatttc 6241 ccctccccct cccctctccc tctccctcgt ccggctctga cctcgccacc ctaccgcggc 6301 ggcggctgct cgcgggcgtc ttgcctcttt cccgtccggc tcttccgtgt ctacgagggg 6361 cggtacgtcg ttacgggttt ttgacccgtc ccgggggcgt tcggtcgtcg gggcgcgcgc 6421 cttgctctcc cggcacccat ccccgccgcg gctctggctt ttctacgttg gctggggcgg 6481 ttgtcgcgtg tggggggatg tgagtgtcgc gtgtgggttc gcccgtcccg atgccacgct 6541 tttctggcct cgcgtgtcct ccccgctcct gtcccgggta cctagctgtc gcgttccggc 6601 gcggaggttt aaagaccccg ggggggtcgc cctgccgccc ccagggtcgg ggggcggtgg 6661 ggcccgtagg gaagtcggtc gttcgggcgg ctctccctca gactccatga ccctcctccc 6721 gccgctgccg ccgttcccga ggcggcggtc gtgtgggggg gtggatgtct ggagccccct 6781 ctgggcgccc gtgggggccc gacccgcgcc gccggcttgc ccgatttccg cgggtcggtc 6841 ctgtcggtgc cggtcgtggg ttcccgtgtc gttcccgtgt ttttccgctc ccgacccttt 6901 tttttccccc ccacacgtgt ctcgtttcgt tcctcgctgg ccggcctgag gctacccctc 6961 gttccgtctg ttctcctctc tctccgggga gaggagggcg gtggtcgttg ggggactgtg 7021 ccgtcgtcag cacccgtgag ttcgctcaca cccgaaatac cgatacgcgg ccgcgtggga 7081 tccccatcct cggcgcgtcc gtcggggacg gccggttgga ggggcgggag gggtttttcc 7141 cgtgaacgcc gcgttcggcg ccaggcctct ggcggccggg ggggcgctct ctccgcccga 7201 gcatccccac tcccgcccct cctcttcgcg cgccgcggcg gcgacgtgcg tacgagggga 7261 ggatgtcgcg gtgtggaggc ggagagggtc cggcgcggcg cctcttccat tttttccccc 7321 ccaacttcgg aggtcgacca gtactccggg cgacactttg tttttttttt ttcccccgat 7381 gctggaggtc gaccagatgt ccgaaagtgt cccccccccc cccggcgcgg agcggcgggg 7441 ccactctgga ctcttttttt tttttttttt tttttaaatt cctggaacct ttaggtcgac 7501 cagttgtccg tcttttactc cttcatatag gtcgaccagt actccgggtg gtactttgtc 7561 tttttctgaa aatcccagag gtcgaccaga tatccgaaag tcctctcttt ccctttactc 7621 ttccccacag cgattctctt tttttttttt tttttggtgt gcctcttttt gacttatata 7681 catgtaaata gtgtgtacgt ttatatactt ataggaggag gtcgaccagt actccgggcg 7741 acactttgtt tttttttcca ccgatgatgg aggtcgacca gatgtccgaa agtgtcccgt 7801 cccccccctc cccccccgcg acgcggcggg ctcactctgg actctttttt tttttttttt 7861 tttaaatttc tggaacctta aggtcgacca gttgtctgtc tttcactcat tcatataggt 7921 cgactggtgg tactttgtct ttttctcaaa atcgcagagg tcgaccagat gtcagaaagt 7981 ctggtggtcg caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt 8041 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatcca ggtggcactt 8101 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 8161 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 8221 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 8281 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 8341 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 8401 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 8461 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 8521 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 8581 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 8641 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 8701 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 8761 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 8821 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 8881 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 8941 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 9001 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 9061 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 9121 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 9181 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 9241 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 9301 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 9361 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 9421 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 9481 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 9541 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 9601 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 9661 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 9721 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 9781 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 9841 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg 9901 ctcgaca //