LOCUS Exported 899 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.. ACCESSION . VERSION . KEYWORDS lentiCRISPR v2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 899) AUTHORS Sanjana NE, Shalem O, Zhang F TITLE Improved vectors and genome-wide libraries for CRISPR screening. JOURNAL Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PUBMED 25075903 REFERENCE 2 (bases 1 to 899) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..899 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 52..173 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(63..82) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 117..136 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(303..677) /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" promoter complement(696..743) /label=EM7 promoter /note="synthetic bacterial promoter " primer_bind complement(859..878) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" ORIGIN 1 gaccatgatt acgccaagct ctagctagag gtcgacggta tacagacatg ataagataca 61 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa 121 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttggggtgg 181 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa 241 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg 301 tgtcagtcct gctcctcggc cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg 361 aactcccgcc cccacggctg ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg 421 aagttcgtgg acacgacctc cgaccactcg gcgtacagct cgtccaggcc gcgcacccac 481 acccaggcca gggtgttgtc cggcaccacc tggtcctgga ccgcgctgat gaacagggtc 541 acgtcgtccc ggaccacacc ggcgaagtcg tcctccacga agtcccggga gaacccgagc 601 cggtcggtcc agaactcgac cgctccggcg acgtcgcgcg cggtgagcac cggaacggca 661 ctggtcaact tggccatggt ttagttcctc accttgtcgt attatactat gccgatatac 721 tatgccgatg attaattgtc aacacgtgct gatcagatcc gaaaatggat atacaagctc 781 ccgggagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata 841 gctcagaggc agaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatgg //