LOCUS Exported 972 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.. ACCESSION . VERSION . KEYWORDS lentiGuide-Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 972) AUTHORS Sanjana NE, Shalem O, Zhang F TITLE Improved vectors and genome-wide libraries for CRISPR screening. JOURNAL Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PUBMED 25075903 REFERENCE 2 (bases 1 to 972) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..972 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 36..635 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(36..55) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 532..552 /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind complement(704..724) /label=WPRE-R /note="WPRE, reverse primer" ORIGIN 1 ttcttccatt tcaggtgtcg tgacgtacgg ccaccatgac cgagtacaag cccacggtgc 61 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg 121 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc 181 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 241 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 301 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 361 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 421 gagtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 481 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 541 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc 601 gcacctggtg catgacccgc aagcccggtg cctgaacgcg ttaagtcgac aatcaacctc 661 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 721 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 781 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 841 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 901 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 961 cggaactcat cg //