LOCUS Exported 837 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.. ACCESSION . VERSION . KEYWORDS lentiGuide-Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 837) AUTHORS Sanjana NE, Shalem O, Zhang F TITLE Improved vectors and genome-wide libraries for CRISPR screening. JOURNAL Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PUBMED 25075903 REFERENCE 2 (bases 1 to 837) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..837 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(5..109) /gene="bla" /label=AmpR promoter rep_origin complement(135..590) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(272..293) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 484..503 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 717..739 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 731..748 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 732..748 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 758..777 /label=T7 /note="T7 promoter, forward primer" promoter 758..776 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 61 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 121 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg 181 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa 241 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa 301 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg 361 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa 421 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa 481 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct 541 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc 601 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 661 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 721 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga 781 attgggtacg tccctaggcc tccaaaaaag cctcctcact acttctggaa tagctca //