LOCUS Exported 11570 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Has ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and crtYm gene of marine bacterium P99-3. and produces gamma-carotene in Escherichia coli. ACCESSION . VERSION . KEYWORDS pAC-GAMMAipi SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11570) AUTHORS Cunningham FX Jr, Gantt E TITLE A study in scarlet: enzymes of ketocarotenoid biosynthesis in the flowers of Adonis aestivalis. JOURNAL Plant J. 2005 Feb;41(3):478-92. PUBMED 15659105 REFERENCE 2 (bases 1 to 11570) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11570 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 134..153 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(220..322) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" primer_bind complement(714..731) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(848..1393) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind complement(928..947) /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind complement(4066..4085) /label=T7 /note="T7 promoter, forward primer" promoter complement(4067..4085) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4095..4112) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4095..4111) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4104..4126) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(10906..10925) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" CDS complement(11130..219) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc 361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat 421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt 481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg 541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact 601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa 661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc 721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc 781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa 841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc 901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc 961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc 1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac 1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt 1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt 1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg 1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt 1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc 1381 aaaacgatct caagaagatc atcttattaa tattgccagc ggttactctg aagaagatac 1441 cattatacaa gattgcttaa agtctctaga ccttgtatta ttaggtgtta tcttttttac 1501 tgttgccatg ggactattcg aattatacgt tgccaaaatc aaaaacttac catcgtggct 1561 cgttattgaa acattagacg atcttaaatc gttactcatt aaaatggtaa tttttgtaat 1621 ggctatttcg tttacgggta aagttgttac atactccgaa ggtattgaaa tactttatct 1681 aggcgcggga ttctcattag ttaccttagc tcttacctat tttataaata ataaataaac 1741 ctgatgtttt acctagacag gtttcgtatt ttgctagctt tatctattga tttcaatgtc 1801 taaacaaatt actactctta ctttttttaa ataccctaaa ttaaaggaca agatatgggc 1861 attttggatg atgcaatttg cgcatagcgc attgcgccga caaagcggat tacagtcata 1921 caaattatta ggaagtggta aagaaggttt tagtccatgg ccagattgga gtgtttacgg 1981 attattacaa gtatgggatt cgcataaaga agcccaagaa ttttttgatt cttctagtct 2041 ttataagaaa tatcttaatc acagcgagca gcaattgacc ttttacatga aaaatattaa 2101 agcatatggc aacgtttagt ctgtgggacg atgcaagggc tttaaaaaag tttgcttaca 2161 gaggtaataa ccataaacaa gccatacagc aaactcaagc attacagtgg tataaagaag 2221 aaatgttctc tagatttcaa ccgtatttga ttacaggaag ttggcagggc ttcacgattc 2281 cagaactgct atcgtttaaa gagacttaaa aaagttcttt acaaagggcc atgaagtaag 2341 tgacttcata attcctaatt cagcaagaag gctgctttct tcatctagaa aagccatgat 2401 attttgtgca ctgttgcgct ggtacatttt actaaatatc tcaggtccag aagcttcatt 2461 ctcgtgtaaa acgcgcagta gagtttcgtc aaaaacttcg tattttttat tagtcatttt 2521 ataggaaaga ggtctgtttt ctagataatt attgaccaat tgtcgggcaa gtcgttcact 2581 atttttaaaa ctataaccag tacttgcctt tacccagccg ccagcagttc ctattttaat 2641 tacattctta tttttaaagg gtttaaaatc atgactcgtc atgggaataa tccctttttc 2701 taccttggtt atcttataat cactaatttt taaaaagtct gtaatatagg tgtcaaggta 2761 ctgatcataa acatcgtctt gcaccagatt tttagcaaag taggtatact ccactagcgc 2821 ctttctttta gaaaacggaa gtatataaac aaagctagtt gttcccggat ctaccagttg 2881 ataatccatt ttagtaaatt cctttgggtc aaaataatct tcagaggttt caatgaacca 2941 acccttgaag tgttgcaaca gtgtaatccc agttgatgga atgccctcta atggtctact 3001 atctagaact aaatctgcgg ttaatgagcc gttattgtat gatacggtta cttctttgga 3061 attatgtgtt tgctctaagt gtgttacttc actcccgatg taatggtatt tggaattttg 3121 ttgtaattgc gctttcgcga aagcggaaaa cttcacattt tcaattgatt tatataggta 3181 aggattcatg gtaagggata attcctcatc tcctgtgtga aatctacatt ttttccaact 3241 ggcaatgatt aagtcgtcat acttaccagg acctttttcc caaaagctcc atgttttttc 3301 ttgagttaaa gacccgtatg gatcaagtat agccactttt aaatgtggcg cattctcgag 3361 caattgtagt actacatgac tacctgcaca acccattcct ataatggcta tatcgtaatg 3421 attatccata acacaattgc aaaaatgagt tgaacgctat ataaatgcag agatattttt 3481 atatctcctt taagattgaa gaattgtaat acatattgca atgcagcagc gattaaaaac 3541 cagcatatat aattgaacca gtctgcacca ttattaaaat gccaaaatcc agccacatca 3601 caaatttgct ctagaaagaa atccagaacc accatgagaa aagcacctgc aagcggaatc 3661 aataaacgtt gagttatgta ttttctactt atttcataag taataaatga taatagtgcc 3721 cagttacttc caataaggta gggtatacca tctaatttcc agcccatgtt aacaccatag 3781 tgataatctc caaacaaata tcctgtgtgt acacctatcc attcaacact cattcctgtg 3841 ataaaacaga ggccaaaaag caatgccttc tttaccgaat tcggcggccg cggatccatc 3901 cttttttctt aagtcgcaag gcagcagcta ggccggcaat tccagaccct ataatgatag 3961 cctttttcat tatttgaaat acttgaatgg cactactagc attccaaaat tttcaccatc 4021 tttttttcct agatgtttgt gatgaatttt gtgagctcca attcgcccta tagtgagtcg 4081 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 4141 acccaactta atcgccttgc agcacatccc cctttcgcca gagctttaat gcggtagttt 4201 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 4261 cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact 4321 gccgggcctc ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct 4381 gctagcgcta tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga 4441 ccgctttggc cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc 4501 gatcatggcg accacacccg tcctgtggat ctaaaggcac agcgtctcat gcttcgcaca 4561 atgtaaaact gcttcagaac ctggcgagag ctatccgcgc ggtctacggt taactgatac 4621 taaaagacaa ttcagcgggt aaccttgcaa tggtgagtgg cagtaaagcg ggcgtttcgc 4681 ctcatcgcga aatagaagta atgagacaat ccattgacga tcacctggct ggcctgttac 4741 ctgaaaccga cagccaggat atcgtcagcc ttgcgatgcg tgaaggcgtc atggcacccg 4801 gtaaacggat ccgtccgctg ctgatgctgc tggccgcccg cgacctccgc taccagggca 4861 gtatgcctac gctgctcgat ctcgcctgcg ccgttgaact gacccatacc gcgtcgctga 4921 tgctcgacga catgccctgc atggacaacg ccgagctgcg ccgcggtcag cccactaccc 4981 acaaaaaatt tggtgagagc gtggcgatcc ttgcctccgt tgggctgctc tctaaagcct 5041 ttggtctgat cgccgccacc ggcgatctgc cgggggagag gcgtgcccag gcggtcaacg 5101 agctctctac cgccgtgggc gtgcagggcc tggtactggg gcagtttcgc gatcttaacg 5161 atgccgccct cgaccgtacc cctgacgcta tcctcagcac caaccacctc aagaccggca 5221 ttctgttcag cgcgatgctg cagatcgtcg ccattgcttc cgcctcgtcg ccgagcacgc 5281 gagagacgct gcacgccttc gccctcgact tcggccaggc gtttcaactg ctggacgatc 5341 tgcgtgacga tcacccggaa accggtaaag atcgcaataa ggacgcggga aaatcgacgc 5401 tggtcaaccg gctgggcgca gacgcggccc ggcaaaagct gcgcgagcat attgattccg 5461 ccgacaaaca cctcactttt gcctgtccgc agggcggcgc catccgacag tttatgcatc 5521 tgtggtttgg ccatcacctt gccgactggt caccggtcat gaaaatcgcc tgataccgcc 5581 cttttgggtt caagcagtac ataacgatgg aaccacatta caggagtagt gatgaatgaa 5641 ggacgagcgc cttgttcagc gtaagaacga tcatctggat atcgttctcg acccccgtcg 5701 cgccgtaact caggctagcg caggttttga gcgctggcgc tttacccact gcgccctgcc 5761 agagctgaat tttagcgaca tcacgctgga aaccaccttc ctgaatcggc agctacaggc 5821 tccgctgctg atcagctcca tgaccggcgg cgttgagcgc tcgcgccata tcaaccgcca 5881 cctcgccgag gcggcgcagg tgctaaaaat tgcgatgggg gtgggctccc agcgcgtcgc 5941 cattgagagc gacgcgggct tagggctgga taaaaccctg cggcagctgg ctccggacgt 6001 gccgctgctg gcgaacctcg gcgcggcgca gctgaccggc agaaaaggta ttgattacgc 6061 ccgacgggcc gtggagatga tcgaggcgga tgcgctgatt gtgcacctaa acccgctgca 6121 ggaggcgcta cagcccggcg gcgatcgcga ctggcgcgga cggctggcgg ctattgaaac 6181 tctggtccgc gagctgcccg ttccgctggt ggtgaaagag gtgggagccg gtatctcccg 6241 aaccgtggcc gggcagctga tcgatgccgg cgttaccgtg attgacgtcg cgggcgcggg 6301 cggcaccagc tgggccgccg ttgaaggcga gcgggcggcc accgagcagc agcgcagcgt 6361 ggccaacgtc tttgccgact gggggatccc caccgctgag gcgctggttg acattgccga 6421 ggcctggccg cagatgcccc ttattgcctc gggcgggatt aaaaacggcg tcgacgcggc 6481 gaaagcgctg cggctcggcg cgtgcatggt agggcaggcc gccgccgtgc tcggcagcgc 6541 aggcgtctcc acggagaagg tgatcgatca cttcaacgtg attattgagc agctgcgggt 6601 ggcctgcttc tgcaccggca gccgcagcct gagcgatcta aagcaggctg atatccgcta 6661 tgtgcgggat acgccatgag ccattttgcc attgtggcac cgccgctcta cagtcatgcg 6721 gtggcgctgc atgccctggc gctggagatg gcccaacgcg gccacatcgt tctcgacccc 6781 cgtcgcgccg taactcaggc tagcgcaggt tttgagcgct ggcgctttac ccactgcgcc 6841 ctgccagagc tgaattttag cgacatcacg ctggaaacca ccttcctgaa tcggcagcta 6901 caggctccgc tgctgatcag ctccatgacc ggcggcgttg agcgctcgcg ccatatcaac 6961 cgccacctcg ccgaggcggc gcaggtgcta aaaattgcga tgggggtggg ctcccagcgc 7021 gtcgccattg agagcgacgc gggcttaggg ctggataaaa ccctgcggca gctggctccg 7081 gacgtgccgc tgctggcgaa cctcggcgcg gcgcagctga ccggcagaaa aggtattgat 7141 tacgcccgac gggccgtgga gatgatcgag gcggatgcgc tgattgtgca cctaaacccg 7201 ctgcaggagg cgctacagcc cggcggcgat cgcgactggc gcggacggct ggcggctatt 7261 gaaactctgg tccgcgagct gcccgttccg ctggtggtga aagaggtggg agccggtatc 7321 tcccgaaccg tggccgggca gctgatcgat gccggcgtta ccgtgattga cgtcgcgggc 7381 gcgggcggca ccagctgggc cgccgttgaa ggcgagcggg cggccaccga gcagcagcgc 7441 agcgtggcca acgtctttgc cgactggggg atccccaccg ctgaggcgct ggttgacatt 7501 gccgaggcct ggccgcagat gccccttatt gcctcgggaa tgcgggctgg gctatttcac 7561 cctaccactg gctattcgct gccgctggcg gtggcccttg ccgacgcgat tgccgacagc 7621 ccgcggctgg gcagcgttcc gctctatcag ctcacccggc agtttgccga acgccactgg 7681 cgcaggcagg gattcttccg cctgctgaac cggatgcttt tcctggccgg gcgcgaggag 7741 aaccgctggc gggtgatgca gcgcttttat gggctgccgg agcccaccgt agagcgcttt 7801 tacgccggtc ggctctctct ctttgataag gcccgcattt tgacgggcaa gccaccggtt 7861 ccgctgggcg aagcctggcg ggcggcgctg aaccattttc ctgacagacg agataaagga 7921 tgaaaaaaac cgttgtgatt ggcgcaggct ttggtggcct ggcgctggcg attcgcctgc 7981 aggcggcagg gatcccaacc gtactgctgg agcagcggga caagcccggc ggtcgggcct 8041 acgtctggca tgaccagggc tttacctttg acgccgggcc gacggtgatc accgatccta 8101 ccgcgcttga ggcgctgttc accctggccg gcaggcgcat ggaggattac gtcaggctgc 8161 tgccggtaaa acccttctac cgactctgct gggagtccgg gaagaccctc gactatgcta 8221 acgacagcgc cgagcttgag gcgcagatta cccagttcaa cccccgcgac gtcgagggct 8281 accggcgctt tctggcttac tcccaggcgg tattccagga gggatatttg cgcctcggca 8341 gcgtgccgtt cctctctttt cgcgacatgc tgcgcgccgg gccgcagctg cttaagctcc 8401 aggcgtggca gagcgtctac cagtcggttt cgcgctttat tgaggatgag catctgcggc 8461 aggccttctc gttccactcc ctgctggtag gcggcaaccc cttcaccacc tcgtccatct 8521 acaccctgat ccacgccctt gagcgggagt ggggggtctg gttccctgag ggcggcaccg 8581 gggcgctggt gaacggcatg gtgaagctgt ttaccgatct gggcggggag atcgaactca 8641 acgcccgggt cgaagagctg gtggtggccg ataaccgcgt aagccaggtc cggctggcgg 8701 atggtcggat ctttgacacc gacgccgtag cctcgaacgc tgacgtggtg aacacctata 8761 aaaagctgct cggccaccat ccggtggggc agaagcgggc ggcagcgctg gagcgcaaga 8821 gcatgagcaa ctcgctgttt gtgctctact tcggcctgaa ccagcctcat tcccagctgg 8881 cgcaccatac catctgtttt ggtccccgct accgggagct gatcgacgag atctttaccg 8941 gcagcgcgct ggcggatgac ttctcgctct acctgcactc gccctgcgtg accgatccct 9001 cgctcgcgcc tcccggctgc gccagcttct acgtgctggc cccggtgccg catcttggca 9061 acgcgccgct ggactgggcg caggaggggc cgaagctgcg cgaccgcatc tttgactacc 9121 ttgaagagcg ctatatgccc ggcctgcgta gccagctggt gacccagcgg atctttaccc 9181 cggcagactt ccacgacacg ctggatgcgc atctgggatc ggccttctcc atcgagccgc 9241 tgctgaccca aagcgcctgg ttccgcccgc acaaccgcga cagcgacatt gccaacctct 9301 acctggtggg cgcaggtact caccctgggg cgggcattcc tggcgtagtg gcctcggcga 9361 aagccaccgc cagcctgatg attgaggatc tgcaatgagc caaccgccgc tgcttgacca 9421 cgccacgcag accatggcca acggctcgaa aagttttgcc accgctgcga agctgttcga 9481 cccggccacc cgccgtagcg tgctgatgct ctacacctgg tgccgccact gcgatgacgt 9541 cattgacgac cagacccacg gcttcgccag cgaggccgcg gcggaggagg aggccaccca 9601 gcgcctggcc cggctgcgca cgctgaccct ggcggcgttt gaaggggccg agatgcagga 9661 tccggccttc gctgcctttc aggaggtggc gctgacccac ggtattacgc cccgcatggc 9721 gctcgatcac ctcgacggct ttgcgatgga cgtggctcag acccgctatg tcacctttga 9781 ggatacgctg cgctactgct atcacgtggc gggcgtggtg ggtctgatga tggccagggt 9841 gatgggcgtg cgggatgagc gggtgctgga tcgcgcctgc gatctggggc tggccttcca 9901 gctgacgaat atcgcccggg atattattga cgatgcggct attgaccgct gctatctgcc 9961 cgccgagtgg ctgcaggatg ccgggctgac cccggagaac tatgccgcgc gggagaatcg 10021 ggccgcgctg gcgcgggtgg cggagcggct tattgatgcc gcagagccgt actacatctc 10081 ctcccaggcc gggctacacg atctgccgcc gcgctgcgcc tgggcgatcg ccaccgcccg 10141 cagcgtctac cgggagatcg gtattaaggt aaaagcggcg ggaggcagcg cctgggatcg 10201 ccgccagcac accagcaaag gtgaaaaaat tgccatgctg atggcggcac cggggcaggt 10261 tattcgggcg aagacgacga gggtgacgcc gcgtccggcc ggtctttggc agcgtcccgt 10321 ttaggcgggc ggccatgacg ttcacgcagg atcgcctgta ggtcggcagg cttgcgggcg 10381 taaataaaac cgaaggagac gcagccctcc cggccgcgca ccgcgtggtg caggcggtgg 10441 gcgacgtaga gccgcttcag gtagccccgg cgcgggatcc cgcctcgccg tgctgtccgg 10501 tctcaacctg atccgccaga atcgagccaa cgggatcggc cagcacgaat tcggtatgcg 10561 gagagtgttt ggcaaaccat gcctgcaagc cacccagcgt gccgccggag ccaacgccta 10621 ctaccacggc atcaaccctg cctgccagct ggtcgaacag ctccggtgcg gtggtggtgg 10681 cgtgcgccag cgggttggcc gggttagaga actgatcaat atagtaagca cccggcgtct 10741 cctctgccag gcggcgggcg tagtcctgat agtactccgg gtggcccttt gtcacgtcgg 10801 agcgggtcag gcgcacatca acacccagcg cacgcaggtg gtagatcctc tacgccggac 10861 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca 10921 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg 10981 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg tcgaatttgc 11041 tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc accaggcgtt 11101 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac 11161 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc 11221 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa 11281 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc 11341 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg 11401 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg 11461 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa 11521 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg //