LOCUS       Exported                9259 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola 
            (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in 
            Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    pAC-BETAipi
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     A study in scarlet: enzymes of ketocarotenoid biosynthesis in the 
            flowers of Adonis aestivalis.
  JOURNAL   Plant J. 2005 Feb;41(3):478-92.
  PUBMED    15659105
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9259
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1505..1533
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8592..8611)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(8819..219)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
     1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
     1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
     1861 ccgtcctgtg gatctaaagg cacagcgtct catgcttcgc acaatgtaaa actgcttcag
     1921 aacctggcga gagctatccg cgcggtctac ggttaactga tactaaaaga caattcagcg
     1981 ggtaaccttg caatggtgag tggcagtaaa gcgggcgttt cgcctcatcg cgaaatagaa
     2041 gtaatgagac aatccattga cgatcacctg gctggcctgt tacctgaaac cgacagccag
     2101 gatatcgtca gccttgcgat gcgtgaaggc gtcatggcac ccggtaaacg gatccgtccg
     2161 ctgctgatgc tgctggccgc ccgcgacctc cgctaccagg gcagtatgcc tacgctgctc
     2221 gatctcgcct gcgccgttga actgacccat accgcgtcgc tgatgctcga cgacatgccc
     2281 tgcatggaca acgccgagct gcgccgcggt cagcccacta cccacaaaaa atttggtgag
     2341 agcgtggcga tccttgcctc cgttgggctg ctctctaaag cctttggtct gatcgccgcc
     2401 accggcgatc tgccggggga gaggcgtgcc caggcggtca acgagctctc taccgccgtg
     2461 ggcgtgcagg gcctggtact ggggcagttt cgcgatctta acgatgccgc cctcgaccgt
     2521 acccctgacg ctatcctcag caccaaccac ctcaagaccg gcattctgtt cagcgcgatg
     2581 ctgcagatcg tcgccattgc ttccgcctcg tcgccgagca cgcgagagac gctgcacgcc
     2641 ttcgccctcg acttcggcca ggcgtttcaa ctgctggacg atctgcgtga cgatcacccg
     2701 gaaaccggta aagatcgcaa taaggacgcg ggaaaatcga cgctggtcaa ccggctgggc
     2761 gcagacgcgg cccggcaaaa gctgcgcgag catattgatt ccgccgacaa acacctcact
     2821 tttgcctgtc cgcagggcgg cgccatccga cagtttatgc atctgtggtt tggccatcac
     2881 cttgccgact ggtcaccggt catgaaaatc gcctgatacc gcccttttgg gttcaagcag
     2941 tacataacga tggaaccaca ttacaggagt agtgatgaat gaaggacgag cgccttgttc
     3001 agcgtaagaa cgatcatctg gatatcgttc tcgacccccg tcgcgccgta actcaggcta
     3061 gcgcaggttt tgagcgctgg cgctttaccc actgcgccct gccagagctg aattttagcg
     3121 acatcacgct ggaaaccacc ttcctgaatc ggcagctaca ggctccgctg ctgatcagct
     3181 ccatgaccgg cggcgttgag cgctcgcgcc atatcaaccg ccacctcgcc gaggcggcgc
     3241 aggtgctaaa aattgcgatg ggggtgggct cccagcgcgt cgccattgag agcgacgcgg
     3301 gcttagggct ggataaaacc ctgcggcagc tggctccgga cgtgccgctg ctggcgaacc
     3361 tcggcgcggc gcagctgacc ggcagaaaag gtattgatta cgcccgacgg gccgtggaga
     3421 tgatcgaggc ggatgcgctg attgtgcacc taaacccgct gcaggaggcg ctacagcccg
     3481 gcggcgatcg cgactggcgc ggacggctgg cggctattga aactctggtc cgcgagctgc
     3541 ccgttccgct ggtggtgaaa gaggtgggag ccggtatctc ccgaaccgtg gccgggcagc
     3601 tgatcgatgc cggcgttacc gtgattgacg tcgcgggcgc gggcggcacc agctgggccg
     3661 ccgttgaagg cgagcgggcg gccaccgagc agcagcgcag cgtggccaac gtctttgccg
     3721 actgggggat ccccaccgct gaggcgctgg ttgacattgc cgaggcctgg ccgcagatgc
     3781 cccttattgc ctcgggcggg attaaaaacg gcgtcgacgc ggcgaaagcg ctgcggctcg
     3841 gcgcgtgcat ggtagggcag gccgccgccg tgctcggcag cgcaggcgtc tccacggaga
     3901 aggtgatcga tcacttcaac gtgattattg agcagctgcg ggtggcctgc ttctgcaccg
     3961 gcagccgcag cctgagcgat ctaaagcagg ctgatatccg ctatgtgcgg gatacgccat
     4021 gagccatttt gccattgtgg caccgccgct ctacagtcat gcggtggcgc tgcatgccct
     4081 ggcgctggag atggcccaac gcggccactc gaccagccgc gctacgttgc cgaggctaat
     4141 ctggtgatca cccacggcgg tctcaatacc gtactggatg cgctggctgc cgcgacgccg
     4201 gtgctggcgg tgccactctc tttcgaccag cccgccgtgg ctgcccggct ggtctataac
     4261 gggctgggtc gccgggtatc gcgctttgcc agacagcaga cgctggcgga tgagattgcc
     4321 caactgctgg gggatgagac gctgcatcag cgtctggcga cggcccgcca gcagcttaac
     4381 gacgccgggg gcacgccccg tgcggcgacc ctgattgaac aggccatagc agggagtgag
     4441 agcgtatcgt gagggatctg attttagtcg gcggcggcct ggccaacggg ctgatcgcct
     4501 ggcgtctgcg ccagcgctac ccgcagctta acctgctgct gatcgaggcc ggggagcagc
     4561 ccggcgggaa ccatacctgg tcattccatg aagacgatct gactcccggg cagcacgcct
     4621 ggctggcccc gctggtggcc cacgcctggc cgggctatga ggtgcagttt cccgatcttc
     4681 gccgtcgcct cgcgcgcggc tactactcca ttacctcaga gcgctttgcc gaggccctgc
     4741 atcaggcgct gggggagaac atctggctaa actgttcggt gagcgaggtg ttacccaata
     4801 gcgtgcgcct tgccaacggt gaggcgctgc ttgccggagc ggtgattgac ggacgcggcg
     4861 tgaccgccag ttcggcgatg caaaccggct atcagctctt tcttggtcag cagtggcggc
     4921 tgacacagcc ccacggcctg accgtaccga tcctgatgga tgccacggtg gcgcagcagc
     4981 agggctatcg ctttgtctac acgctgccgc tctccgccga cacgctgctg atcgaggata
     5041 cgcgctacgc caatgtcccg cagcgtgatg ataatgccct acgccagacg gttaccgact
     5101 atgctcacag caaagggtgg cagctggccc agcttgaacg cgaggagacc ggctgtctgc
     5161 cgattaccct ggcgggtgac atccaggctc tgtgggccga tgcgccgggc gtgccgcgct
     5221 cgggaatgcg ggctgggcta tttcacccta ccactggcta ttcgctgccg ctggcggtgg
     5281 cccttgccga cgcgattgcc gacagcccgc ggctgggcag cgttccgctc tatcagctca
     5341 cccggcagtt tgccgaacgc cactggcgca ggcagggatt cttccgcctg ctgaaccgga
     5401 tgcttttcct ggccgggcgc gaggagaacc gctggcgggt gatgcagcgc ttttatgggc
     5461 tgccggagcc caccgtagag cgcttttacg ccggtcggct ctctctcttt gataaggccc
     5521 gcattttgac gggcaagcca ccggttccgc tgggcgaagc ctggcgggcg gcgctgaacc
     5581 attttcctga cagacgagat aaaggatgaa aaaaaccgtt gtgattggcg caggctttgg
     5641 tggcctggcg ctggcgattc gcctgcaggc ggcagggatc ccaaccgtac tgctggagca
     5701 gcgggacaag cccggcggtc gggcctacgt ctggcatgac cagggcttta cctttgacgc
     5761 cgggccgacg gtgatcaccg atcctaccgc gcttgaggcg ctgttcaccc tggccggcag
     5821 gcgcatggag gattacgtca ggctgctgcc ggtaaaaccc ttctaccgac tctgctggga
     5881 gtccgggaag accctcgact atgctaacga cagcgccgag cttgaggcgc agattaccca
     5941 gttcaacccc cgcgacgtcg agggctaccg gcgctttctg gcttactccc aggcggtatt
     6001 ccaggaggga tatttgcgcc tcggcagcgt gccgttcctc tcttttcgcg acatgctgcg
     6061 cgccgggccg cagctgctta agctccaggc gtggcagagc gtctaccagt cggtttcgcg
     6121 ctttattgag gatgagcatc tgcggcaggc cttctcgttc cactccctgc tggtaggcgg
     6181 caaccccttc accacctcgt ccatctacac cctgatccac gcccttgagc gggagtgggg
     6241 ggtctggttc cctgagggcg gcaccggggc gctggtgaac ggcatggtga agctgtttac
     6301 cgatctgggc ggggagatcg aactcaacgc ccgggtcgaa gagctggtgg tggccgataa
     6361 ccgcgtaagc caggtccggc tggcggatgg tcggatcttt gacaccgacg ccgtagcctc
     6421 gaacgctgac gtggtgaaca cctataaaaa gctgctcggc caccatccgg tggggcagaa
     6481 gcgggcggca gcgctggagc gcaagagcat gagcaactcg ctgtttgtgc tctacttcgg
     6541 cctgaaccag cctcattccc agctggcgca ccataccatc tgttttggtc cccgctaccg
     6601 ggagctgatc gacgagatct ttaccggcag cgcgctggcg gatgacttct cgctctacct
     6661 gcactcgccc tgcgtgaccg atccctcgct cgcgcctccc ggctgcgcca gcttctacgt
     6721 gctggccccg gtgccgcatc ttggcaacgc gccgctggac tgggcgcagg aggggccgaa
     6781 gctgcgcgac cgcatctttg actaccttga agagcgctat atgcccggcc tgcgtagcca
     6841 gctggtgacc cagcggatct ttaccccggc agacttccac gacacgctgg atgcgcatct
     6901 gggatcggcc ttctccatcg agccgctgct gacccaaagc gcctggttcc gcccgcacaa
     6961 ccgcgacagc gacattgcca acctctacct ggtgggcgca ggtactcacc ctggggcggg
     7021 cattcctggc gtagtggcct cggcgaaagc caccgccagc ctgatgattg aggatctgca
     7081 atgagccaac cgccgctgct tgaccacgcc acgcagacca tggccaacgg ctcgaaaagt
     7141 tttgccaccg ctgcgaagct gttcgacccg gccacccgcc gtagcgtgct gatgctctac
     7201 acctggtgcc gccactgcga tgacgtcatt gacgaccaga cccacggctt cgccagcgag
     7261 gccgcggcgg aggaggaggc cacccagcgc ctggcccggc tgcgcacgct gaccctggcg
     7321 gcgtttgaag gggccgagat gcaggatccg gccttcgctg cctttcagga ggtggcgctg
     7381 acccacggta ttacgccccg catggcgctc gatcacctcg acggctttgc gatggacgtg
     7441 gctcagaccc gctatgtcac ctttgaggat acgctgcgct actgctatca cgtggcgggc
     7501 gtggtgggtc tgatgatggc cagggtgatg ggcgtgcggg atgagcgggt gctggatcgc
     7561 gcctgcgatc tggggctggc cttccagctg acgaatatcg cccgggatat tattgacgat
     7621 gcggctattg accgctgcta tctgcccgcc gagtggctgc aggatgccgg gctgaccccg
     7681 gagaactatg ccgcgcggga gaatcgggcc gcgctggcgc gggtggcgga gcggcttatt
     7741 gatgccgcag agccgtacta catctcctcc caggccgggc tacacgatct gccgccgcgc
     7801 tgcgcctggg cgatcgccac cgcccgcagc gtctaccggg agatcggtat taaggtaaaa
     7861 gcggcgggag gcagcgcctg ggatcgccgc cagcacacca gcaaaggtga aaaaattgcc
     7921 atgctgatgg cggcaccggg gcaggttatt cgggcgaaga cgacgagggt gacgccgcgt
     7981 ccggccggtc tttggcagcg tcccgtttag gcgggcggcc atgacgttca cgcaggatcg
     8041 cctgtaggtc ggcaggcttg cgggcgtaaa taaaaccgaa ggagacgcag ccctcccggc
     8101 cgcgcaccgc gtggtgcagg cggtgggcga cgtagagccg cttcaggtag ccccggcgcg
     8161 ggatcccgcc tcgccgtgct gtccggtctc aacctgatcc gccagaatcg agccaacggg
     8221 atcggccagc acgaattcgg tatgcggaga gtgtttggca aaccatgcct gcaagccacc
     8281 cagcgtgccg ccggagccaa cgcctactac cacggcatca accctgcctg ccagctggtc
     8341 gaacagctcc ggtgcggtgg tggtggcgtg cgccagcggg ttggccgggt tagagaactg
     8401 atcaatatag taagcacccg gcgtctcctc tgccaggcgg cgggcgtagt cctgatagta
     8461 ctccgggtgg ccctttgtca cgtcggagcg ggtcaggcgc acatcaacac ccagcgcacg
     8521 caggtggtag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     8581 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     8641 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     8701 gggcgccatc tccttggggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta
     8761 tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt
     8821 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     8881 ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     8941 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     9001 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     9061 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     9121 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     9181 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     9241 cgtctttcat tgccatacg
//
