LOCUS Exported 1126 bp ds-DNA linear SYN 26-MAY-2021 DEFINITION This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.. ACCESSION . VERSION . KEYWORDS pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1126) AUTHORS Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA TITLE Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. JOURNAL Proc Natl Acad Sci U S A. 2013 Aug 12. PUBMED 23940360 REFERENCE 2 (bases 1 to 1126) AUTHORS . TITLE Direct Submission JOURNAL Exported May 26, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1126 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(33..59) /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" ORIGIN 1 tccagcttct ttttcttcgc tgctggagct gcagcgtaat ctggaacatc gtatgggtaa 61 cggacaggcg ggcgtttttt cagacggcat ggtctgattt ctttgcccag ttcgtcaatt 121 tggtattttt ggaatgaaag ggcggttttg acgccgatac cttccagtat tccatttttg 181 ccaattttat gatcaagatc atgaatgcgt atattgatat tacctgtgcc tcgatggcag 241 ctggcaaagt aaccaaacat tctagctttt tttgttataa cctcgactaa atcattaggg 301 tgtaatgaga atttaaagtt gaaactatca tcaataagtt gccaatcttc ttcatctttt 361 ccttgtacaa cagccctatc cggcaaaatc cctttcgcta cctgccaact gtaaatcggt 421 accagataat acttgtcgcc tttctcaaac acatctacgc gcaccatggt tgcgttgtcg 481 gcaataccgt tatggttgcg cacccatacg ccggttttct gtacttgctc tacgcgtacg 541 gcttttacct gttgggtgcg gttgcctgct ttatcgtatt tgtaaaacgg ctcggcaaag 601 gctttggcag gatcgtcttt atgtgcttcc agccgtgctt tcagtgcttc gtatagctta 661 ggttcgcgct cccgattgac cattttttcc aagtctttca gttttaactg tgtcagcggt 721 acgcgcaaca cgctgacgcc ttcgtccagt cgtttggcgg atttgacggt ctccatatgc 781 ccttgcccgc tcatcttccg attgggcgca cgtgaaacaa acagtggcgt aacgtattcg 841 tgtacggctt cggggcgaga tgataatttt tcggcaagca acgtgcgcag tttttctagg 901 gtatcggctt cttcgaattc gggtttgccg tccggtttgc cgaagacgcg aatcatgact 961 tcttgtgcga aaaattccca aggttgtggg aagtgtgttt tttgatgcag cacttctcct 1021 gtttctttgt ctatggtttt accgtcaaac gcgttcatct ctttatagcg tacaaaacgg 1081 gtaattttct gctgcatggc aacggtcgag caggcaacga cgacgg //