LOCUS Exported 842 bp ds-DNA linear SYN 09-JUN-2021 DEFINITION For C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtA. ACCESSION . VERSION . KEYWORDS pFA6a-hphMX-(3×FLAG)-TEV-ProtA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 842) TITLE various JOURNAL Unpublished REFERENCE 2 (bases 1 to 842) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 9, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..842 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 45..116 /codon_start=1 /product="three tandem FLAG(R) epitope tags" /label=3xFLAG /translation="DYKDDDDKDYKDDDDKDYKDDDDK" CDS 144..164 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS 204..377 /codon_start=1 /product="IgG-binding unit of Staphylococcus aureus protein A" /label=ProtA /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL AEAKKLNDAQAPK" CDS 381..551 /codon_start=1 /product="IgG-binding unit of Staphylococcus aureus protein A" /label=ProtA /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA EAKKLNGAQAPK" terminator 605..792 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(660..684) /label=pHybLex-R /note="ADH terminator, reverse primer" ORIGIN 1 gtcgacggat ccccgggtta attaatatgt ctttaatcaa tatggactac aaggacgacg 61 atgacaagga ttacaaagat gacgacgata aggactataa ggacgatgat gataaagatt 121 atgatattcc aactactgct agcgagaatt tgtattttca gggtgagctc aaaaccgcgg 181 ctcttgcgca acacgatgaa gccgtggaca acaaattcaa caaagaacaa caaaacgcgt 241 tctatgagat cttacattta cctaacttaa acgaagaaca acgaaacgcc ttcatccaaa 301 gtttaaaaga tgacccaagc caaagcgcta accttttagc agaagctaaa aagctaaatg 361 atgctcaggc gccgaaagta gacaacaaat tcaacaaaga acaacaaaac gcgttctatg 421 agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa 481 aagatgaccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta aatggtgctc 541 aggcgccgaa agtagacgcg aattccgcgg ggaagtcaac ctgaggcgcg ccacttctaa 601 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag 661 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa 721 ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca 781 cacctctacc ggcagatccg ctagggataa cagggtaata tagatctgtt tagcttgcct 841 cg //