LOCUS       Exported                5138 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Produces an active CBFD (carotenoid beta-ring 4-dehydrogenase) 
            enzyme in E. coli when expression is induced with arabinose.
ACCESSION   .
VERSION     .
KEYWORDS    pCBFD2Bad
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     Elucidation of the pathway to astaxanthin in the flowers of Adonis 
            aestivalis.
  JOURNAL   Plant Cell. 2011 Aug;23(8):3055-69. doi: 10.1105/tpc.111.086827. 
            Epub 2011 Aug 23.
  PUBMED    21862704
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             312..334
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1539..1556)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1709..1795
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1887..1914
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1933..2024
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2025..2885
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2243..2262)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3056..3644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3545..3564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3798..3815
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3830..3970
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3896..3915)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4056..4078
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4234..5112)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctagaaataa ttttgtttaa ctttaagaag gagatataca tacccatggc agcagcaatt
      361 tcagtgttca gttcaggtta ttctttctac aagaatctct tgttggactc aaaaccaaat
      421 attctcaaac ccccatgcct gctattctct ccagttgtga tcatgtcgcc tatgagaaag
      481 aaaaagaaac atggtgatcc atgtatctgc tccgttgcag ggagaacaag gaaccttgat
      541 attcctcaaa ttgaagaaga ggaagagaat gtggaagaac taatagaaca gaccgattct
      601 gacatagtgc atataaagaa aacactaggg gggaaacaat caaaacggcc cactggctcc
      661 attgtcgcac ccgtatcttg tcttgggatc ctttcaatga ttggacctgc tgtttacttc
      721 aagttttcac ggctaatgga gggtggagat atacctgtag cagaaatggg gattacgttt
      781 gccacctttg ttgctgctgc tgttggcacg gagtttttgt cagcatgggt tcacaaagaa
      841 ctctggcacg agtctttgtg gtacattcac aagtctcacc atcggtcacg aaaaggccgc
      901 ttcgagttca atgatgtgtt tgctattatt aacgcgcttc ccgctattgc tcttatcaat
      961 tatggattct ccaatgaagg cctccttcct ggagcgtgct ttggtgtcgg tcttggaaca
     1021 acagtctgtg gtatggctta catttttctt cacaatggcc tatcacaccg aaggttccca
     1081 gtatggctta ttgcgaacgt cccttatttc cacaagctgg ctgcagctca ccaaatacac
     1141 cactcaggaa aatttcaggg tgtaccattt ggcctgttcc ttggacccaa ggaattggaa
     1201 gaagtaagag gaggcactga agagttggag agggtaatca gtcgtacaac taaacgaacg
     1261 caaccatcta cctgaatcaa tttttttaca tatataaggt tttagtttat cggtgttata
     1321 aaatcacaca tccgtatcgt tttagtaagt caaagttaag atacttcctt cttagaatat
     1381 tttttgatgt ataggtcgcg gatatactgt tacactattc gttgtggaat tccattataa
     1441 aaaaataaaa aaaaaaaaaa aaaaactcga gatctgcaga tggtaccata tgggaattcg
     1501 aagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa
     1561 cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct
     1621 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
     1681 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1741 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     1801 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
     1861 ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt
     1921 tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     2281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3181 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     3241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     3841 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     3901 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     3961 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     4021 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     4081 ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg
     4141 gcatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc
     4201 cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg ctacatcatt
     4261 cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa
     4321 tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg
     4381 catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct
     4441 taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca
     4501 aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta
     4561 ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc
     4621 catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc
     4681 ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc
     4741 ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg
     4801 ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg
     4861 acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac
     4921 aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata
     4981 acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg
     5041 cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg
     5101 cgcttcagcc atacttttca tactcccgcc attcagag
//
