LOCUS Exported 8353 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and pds (crtP) gene of Synechococcus PCC7942, and thereby produces zeta-carotene in E. coli. ACCESSION . VERSION . KEYWORDS pAC-ZETAipi SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8353) AUTHORS Cunningham FX Jr, Gantt E TITLE A portfolio of plasmids for identification and analysis of carotenoid pathway enzymes: Adonis aestivalis as a case study. JOURNAL Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17. PUBMED 17634749 REFERENCE 2 (bases 1 to 8353) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8353 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 134..153 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(220..322) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" primer_bind complement(714..731) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(848..1393) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind complement(928..947) /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 1505..1533 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" protein_bind 4124..4145 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 4160..4190 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4198..4214 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 4203..4225 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 4222..4238 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 4222..4238 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 4257..4277 /label=T3 /note="T3 promoter, forward primer" promoter 4259..4277 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" regulatory 5425..5434 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS complement(7913..219) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc 361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat 421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt 481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg 541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact 601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa 661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc 721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc 781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa 841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc 901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc 961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc 1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac 1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt 1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt 1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg 1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt 1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc 1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca 1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc 1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct 1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg 1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg 1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt 1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc 1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac 1861 ccgtcctgtg gatctaaagg cacagcgtct catgcttcgc acaatgtaaa actgcttcag 1921 aacctggcga gagctatccg cgcggtctac ggttaactga tactaaaaga caattcagcg 1981 ggtaaccttg caatggtgag tggcagtaaa gcgggcgttt cgcctcatcg cgaaatagaa 2041 gtaatgagac aatccattga cgatcacctg gctggcctgt tacctgaaac cgacagccag 2101 gatatcgtca gccttgcgat gcgtgaaggc gtcatggcac ccggtaaacg gatccgtccg 2161 ctgctgatgc tgctggccgc ccgcgacctc cgctaccagg gcagtatgcc tacgctgctc 2221 gatctcgcct gcgccgttga actgacccat accgcgtcgc tgatgctcga cgacatgccc 2281 tgcatggaca acgccgagct gcgccgcggt cagcccacta cccacaaaaa atttggtgag 2341 agcgtggcga tccttgcctc cgttgggctg ctctctaaag cctttggtct gatcgccgcc 2401 accggcgatc tgccggggga gaggcgtgcc caggcggtca acgagctctc taccgccgtg 2461 ggcgtgcagg gcctggtact ggggcagttt cgcgatctta acgatgccgc cctcgaccgt 2521 acccctgacg ctatcctcag caccaaccac ctcaagaccg gcattctgtt cagcgcgatg 2581 ctgcagatcg tcgccattgc ttccgcctcg tcgccgagca cgcgagagac gctgcacgcc 2641 ttcgccctcg acttcggcca ggcgtttcaa ctgctggacg atctgcgtga cgatcacccg 2701 gaaaccggta aagatcgcaa taaggacgcg ggaaaatcga cgctggtcaa ccggctgggc 2761 gcagacgcgg cccggcaaaa gctgcgcgag catattgatt ccgccgacaa acacctcact 2821 tttgcctgtc cgcagggcgg cgccatccga cagtttatgc atctgtggtt tggccatcac 2881 cttgccgact ggtcaccggt catgaaaatc gcctgatacc gcccttttgg gttcaagcag 2941 tacataacga tggaaccaca ttacaggagt agtgatgaat gaaggacgag cgccttgttc 3001 agcgtaagaa cgatcatctg gatatcgttc tcgacccccg tcgcgccgta actcaggcta 3061 gcgcaggttt tgagcgctgg cgctttaccc actgcgccct gccagagctg aattttagcg 3121 acatcacgct ggaaaccacc ttcctgaatc ggcagctaca ggctccgctg ctgatcagct 3181 ccatgaccgg cggcgttgag cgctcgcgcc atatcaaccg ccacctcgcc gaggcggcgc 3241 aggtgctaaa aattgcgatg ggggtgggct cccagcgcgt cgccattgag agcgacgcgg 3301 gcttagggct ggataaaacc ctgcggcagc tggctccgga cgtgccgctg ctggcgaacc 3361 tcggcgcggc gcagctgacc ggcagaaaag gtattgatta cgcccgacgg gccgtggaga 3421 tgatcgaggc ggatgcgctg attgtgcacc taaacccgct gcaggaggcg ctacagcccg 3481 gcggcgatcg cgactggcgc ggacggctgg cggctattga aactctggtc cgcgagctgc 3541 ccgttccgct ggtggtgaaa gaggtgggag ccggtatctc ccgaaccgtg gccgggcagc 3601 tgatcgatgc cggcgttacc gtgattgacg tcgcgggcgc gggcggcacc agctgggccg 3661 ccgttgaagg cgagcgggcg gccaccgagc agcagcgcag cgtggccaac gtctttgccg 3721 actgggggat ccccaccgct gaggcgctgg ttgacattgc cgaggcctgg ccgcagatgc 3781 cccttattgc ctcgggcggg attaaaaacg gcgtcgacgc ggcgaaagcg ctgcggctcg 3841 gcgcgtgcat ggtagggcag gccgccgccg tgctcggcag cgcaggcgtc tccacggaga 3901 aggtgatcga tcacttcaac gtgattattg agcagctgcg ggtggcctgc ttctgcaccg 3961 gcagccgcag cctgagcgat ctaaagcagg ctgatatccg ctatgtgcgg gatacgccat 4021 gagccatttt gccattgtgg caccgccgct ctacagtcat gcggtggcgc tgctggcacg 4081 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 4141 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 4201 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa 4261 ttaaccctca ctaaagggaa caaaagctgg attcttctgc ccttccagcc aaaagccatg 4321 cgcgtagcga tcgccggtgc cggacttgcc ggactctcct gtgccaagta cttggccgat 4381 gccggtcata cgcccatcgt ctatgaacgt cgggacgtcc ttggcggcaa ggttgccgct 4441 tggaaagatg aagacggcga ctggtacgaa actggcctac atatcttttt tggggcttac 4501 cccaacatgt tgcagctctt taaggagctg aacattgaag atcgcctgca gtggaagtcc 4561 cactcgatga tcttcaacca acccacaaag ccgggcacct attcgcgctt cgacttccca 4621 gacattccag cgccaatcaa cggtgttgca gcaatcctca gcaacaacga catgttgacc 4681 tgggaagaaa aaatcaagtt tggcttgggc ttgttgccag cgatgattcg cggccagtcc 4741 tacgtcgaag agatggatca atactcatgg acggagtggc tgcgcaaaca aaatattcca 4801 gagcgggtca acgatgaagt cttcatcgcc atggctaaag cgctcaactt tattgacccg 4861 gacgaaattt ccgccacggt cgtcctaacg gcactcaacc gcttcttgca agagaagaaa 4921 ggttcaatga tggccttttt ggatggtgcg ccgcccgagc gtctttgcca gccgatcgtc 4981 gaacatgtcc aagctcgcgg tggtgatgtg ctgctgaatg cgcctctgaa agagttcgtg 5041 ctcaatgacg acagtagcgt ccaagctttt cggattgctg gcatcaaagg tcaagaagaa 5101 caactcattg aggcagatgc ctacgtttcg gcactgccgg ttgatccgct caagctactg 5161 ttgccggatg catggaaagc catgccctac ttccagcaac tcgatggtct gcagggcgtg 5221 ccggtcatca acattcacct ctggttcgat cgcaagctga ccgatatcga tcacctgctg 5281 ttctcgcgat cgcccctgct cagtgtctat gccgacatga gtaacacctg tcgcgagtac 5341 gaagatcccg atcgctcaat gctagagctg gtcttcgccc ccgccaaaga ctggattggc 5401 cgctccgacg aagacatctt ggctgccacc atggccgaga ttgaaaagct attcccacag 5461 catttcagcg gtgagaatcc ggcacgtctg cgcaaataca aaattgtcaa aacgcccctg 5521 tcggtctaca aagccacgcc gggccgtcaa caatatcgcc ccgatcaagc tagcccgatc 5581 gctaatttct tcctgaccgg cgactacacc atgcagcgct acctcgccag tatggaaggg 5641 gcggtcctat ctggtaagct gacagcgcaa gccatcattg ctcgccaaga tgagttgcaa 5701 cgtcgcagca gcggacgacc gctggccgcg agtcaggcat agacactgtt acccatgttg 5761 cagttgctag ctctgaagct tcactggtca ccctctcacc cccgactcgc accgtctgtc 5821 tgatgctgca gatgataccg gcacggcctt cacgggcgct cgcctctctg tcggaggcct 5881 acgaggagtg ccgacaaatt accgctcgct acgctaagac cttttacctc gggacgcttt 5941 tgatgcccga ggccaagcgt caggccatct gggccatcta cgtctggtgt cgtcgtaccg 6001 atgaactagt cgatggccct caagcagcgc agactaactt cgcgaccctg gatgcttggg 6061 agcggcggtt ggaacgactg tttgctgggg aacccgaaga cgactgcgat gttgccttgg 6121 tggatacctt ggctcgctat cctctcgata tccagccctt ccgcgacatg atcgaagggc 6181 agcggatgga tctgctgcaa aaccgctatt ccacttttga ggatctctac acctactgct 6241 accgtgtcgc cggtactgtg ggactgatgt cacagccggt gatgggcatt gaatcgacca 6301 atagtcgtgc cccttgggat ctttaccggc agcgcgctgg cggatgactt ctcgctctac 6361 ctgcactcgc cctgcgtgac cgatccctcg ctcgcgcctc ccggctgcgc cagcttctac 6421 gtgctggccc cggtgccgca tcttggcaac gcgccgctgg actgggcgca ggaggggccg 6481 aagctgcgcg accgcatctt tgactacctt gaagagcgct atatgcccgg cctgcgtagc 6541 cagctggtga cccagcggat ctttaccccg gcagacttcc acgacacgct ggatgcgcat 6601 ctgggatcgg ccttctccat cgagccgctg ctgacccaaa gcgcctggtt ccgcccgcac 6661 aaccgcgaca gcgacattgc caacctctac ctggtgggcg caggtactca ccctggggcg 6721 ggcattcctg gcgtagtggc ctcggcgaaa gccaccgcca gcctgatgat tgaggatctg 6781 caatgagcca accgccgctg cttgaccacg ccacgcagac catggccaac ggctcgaaaa 6841 gttttgccac cgctgcgaag ctgttcgacc cggccacccg ccgtagcgtg ctgatgctct 6901 acacctggtg ccgccactgc gatgacgtca ttgacgacca gacccacggc ttcgccagcg 6961 aggccgcggc ggaggaggag gccacccagc gcctggcccg gctgcgcacg ctgaccctgg 7021 cggcgtttga aggggccgag atgcaggatc cggccttcgc tgcctttcag gaggtggcgc 7081 tgacccacgg tattacgccc cgcatggcgc tcgatcacct cgacggcttt gcgatggacg 7141 tggctcagac ccgctatgtc acctttgagg atacgctgcg ctactgctat cacgtggcgg 7201 gcgtggtggg tctgatgatg gccagggtga tgggcgtgcg ggatgagcgg gtgctggatc 7261 gcgcctgcga tctggggctg gccttccagc tgacgaatat cgcccgggat attattgacg 7321 atgcggctat tgaccgctgc tatctgcccg ccgagtggct gcaggatgcc gggctgaccc 7381 cggagaacta tgccgcgcgg gagaatcggg ccgcgctggc gcgggtggcg gagcggctta 7441 ttgatgccgc agagccgtac tacatctcct cccaggccgg gctacacgat ctgccgccgc 7501 gctgcgcctg ggcgatcgcc accgcccgca gcgtctaccg ggagatcggt attaaggtaa 7561 aagcggcggg aggcagcgcc tgggatcgcc gccagcacac cagcaaaggt gaaaaaattg 7621 ccatgctgat ggcggcaccg gggcaggtta ttcgggcgaa gacgacgagg gtgacgccgc 7681 gtccggccgg tctttggcag cgtcccgttt aggcgggcgg ccatgacgtt cacgcaggat 7741 cgcctgtagg tcggcaggct tgcgggcgta aataaaaccg aaggagacgc agccctcccg 7801 gccgcgcacg ggtcgaattt gctttcgaat ttctgccatt catccgctta ttatcactta 7861 ttcaggcgta gcaaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc 7921 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc 7981 catcacagac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg 8041 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta 8101 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa 8161 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt 8221 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt 8281 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt 8341 tcattgccat acg //