LOCUS Exported 1654 bp ds-DNA linear SYN 31-MAY-2021 DEFINITION Localization: Membrane Receptor, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-ATG5-N-14 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1654) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 1654) AUTHORS . TITLE Direct Submission JOURNAL Exported May 31, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1654 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 904..913 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 910..1629 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(955..976) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1216..1235) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1563..1584 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 tcagatccgc tagcgctacc ggactcagat ctcgagaccg ccatgacaga tgacaaagat 61 gtgcttcgag atgtgtggtt tggacgaatt ccaacttgtt tcacgctata tcaggatgag 121 ataactgaaa gggaagcaga accatactat ttgcttttgc caagagtaag ttatttgacg 181 ttggtgactg acaaagtgaa aaagcacttt cagaaggtta tgagacaaga agacattagt 241 gagatatggt ttgaatatga aggcacacca ctgaaatggc attatccaat tggtttgcta 301 tttgatcttc ttgcatcaag ttcagctctt ccttggaaca tcacagtaca ttttaagagt 361 tttccagaaa aagaccttct gcactgtcca tctaaggatg caattgaagc tcattttatg 421 tcatgtatga aagaagctga tgctttaaaa cataaaagtc aagtaatcaa tgaaatgcag 481 aaaaaagatc acaagcaact ctggatggga ttgcaaaatg acagatttga ccagttttgg 541 gccatcaatc ggaaactcat ggaatatcct gcagaagaaa atggatttcg ttatatcccc 601 tttagaatat atcagacaac gactgaaaga cctttcattc agaagctgtt tcgtcctgtg 661 gctgcagatg gacagttgca cacactagga gatctcctca aagaagtttg tccttctgct 721 attgatcctg aagatgggga aaaaaagaat caagtgatga ttcatggaat tgagccaatg 781 ttggaaacac ctctgcagtg gctgagtgaa catctgagct acccggataa ttttcttcat 841 attagtatca tcccacagcc aacagatggg agtggcagtc gcggacagag tggtggaccg 901 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 961 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 1021 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 1081 tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac 1141 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 1201 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 1261 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 1321 ctggggcaca agctggagta caactacaac agccacaagg tctatatcac cgccgacaag 1381 cagaagaacg gcatcaaggt gaacttcaag acccgccaca acatcgagga cggcagcgtg 1441 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 1501 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat 1561 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 1621 tacaagtaag cggccgcgac tctagatcat aatc //