LOCUS       Exported                1562 bp ds-DNA     linear   SYN 28-MAY-2021
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    mEmerald-CD81-10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   He J, Sun E, Bujny MV, Kim D, Davidson MW, Zhuang X
  TITLE     Dual function of CD81 in influenza virus uncoating and budding.
  JOURNAL   PLoS Pathog. 2013;9(10):e1003701. doi: 10.1371/journal.ppat.1003701.
            Epub 2013 Oct 10.
  PUBMED    24130495
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 28, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      21..30
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             27..743
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(72..93)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(333..352)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     680..701
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
       61 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      121 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      181 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct
      241 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      301 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      361 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      421 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct
      481 atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca
      541 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      601 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
      661 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      721 tcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tccggagtgg
      781 agggctgcac caagtgcatc aagtacctgc tcttcgtctt caatttcgtc ttctggctgg
      841 ctggaggcgt gatcctgggt gtggccctgt ggctccgcca tgacccgcag accaccaacc
      901 tcctgtatct ggagctggga gacaagcccg cgcccaacac cttctatgta ggcatctaca
      961 tcctcatcgc tgtgggcgct gtcatgatgt tcgttggctt cctgggctgc tacggggcca
     1021 tccaggaatc ccagtgcctg ctggggacgt tcttcacctg cctggtcatc ctgtttgcct
     1081 gtgaggtggc cgccggcatc tggggctttg tcaacaagga ccagatcgcc aaggatgtga
     1141 agcagttcta tgaccaggcc ctacagcagg ccgtggtgga tgatgacgcc aacaacgcca
     1201 aggctgtggt gaagaccttc cacgagacgc ttgactgctg tggctccagc acactgactg
     1261 ctttgaccac ctcagtgctc aagaacaatt tgtgtccctc gggcagcaac atcatcagca
     1321 acctcttcaa ggaggactgc caccagaaga tcgatgacct cttctccggg aagctgtacc
     1381 tcatcggcat tgctgccatc gtggtcgctg tgatcatgat cttcgagatg atcctgagca
     1441 tggtgctgtg ctgtggcatc cggaacagct ccgtgtactg aggcggatcc aagggcgaat
     1501 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
     1561 ag
//
