LOCUS Exported 1275 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION Localization: Nucleus, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-CENPA-C-22 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1275) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 1275) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1275 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 28..37 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 34..750 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(79..100) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(340..359) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 687..708 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc 61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg 241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 481 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc 541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc 661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 721 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg 781 aattctagca gttctagagt aagcagcaga gtaagtggcc cgcgccgccg gagccgaaag 841 cccgaggccc cgaggaggcg cagcccgagc ccgaccccga cccccggccc ctcccggcgg 901 ggcccctcct taggcgcttc ctcccatcaa cacagtcggc ggagacaagg ttggctaaag 961 gagatccgaa agcttcagaa gagcacacac ctcttgataa ggaagctgcc cttcagccgc 1021 ctggcaagag aaatatgtgt taaattcact cgtggtgtgg acttcaattg gcaagcccag 1081 gccctattgg ccctacaaga ggcagcagaa gcatttctag ttcatctctt tgaggacgcc 1141 tatctcctca ccttacatgc aggccgagtt actctcttcc caaaggatgt gcaactggcc 1201 cggaggatcc ggggccttga ggagggactc ggctaaggta ccgcgggccc gggatccacc 1261 ggatctagat aactg //