LOCUS Exported 16103 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS CTV SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 16103) AUTHORS Thai TH, Calado DP, Casola S, Ansel KM, Xiao C, Xue Y, Murphy A, Frendewey D, Valenzuela D, Kutok JL, Schmidt-Supprian M, Rajewsky N, Yancopoulos G, Rao A, Rajewsky K TITLE Regulation of the germinal center response by microRNA-155. JOURNAL Science. 2007 Apr 27. 316(5824):604-8. PUBMED 17463289 REFERENCE 2 (bases 1 to 16103) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..16103 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 5..1086 /label=Rosa26 left arm /note="left homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)" misc_feature 1093..1196 /label=Ad SA /note="adenovirus major late transcript splice acceptor (Friedrich and Soriano, 1991)" enhancer 1210..1589 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" intron 1865..2881 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 2805..2827 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 2889..2908 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" protein_bind 2950..2983 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 2990..3038 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" CDS complement(3045..3848) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3156..3175) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 3766..3785 /label=Neo-R /note="Neomycin resistance gene, reverse primer" intron 4662..4727 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4857..4877 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 5302..5436 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5339..5358) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5393..5412 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" protein_bind 5456..5489 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind 5522..5569 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." misc_feature 5608..6159 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5752..5769) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5979..5998 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6171..6890 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6171..6887 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6216..6237) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6477..6496) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6824..6845 /label=EGFP-C /note="EGFP, forward primer" protein_bind 6915..6962 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." primer_bind complement(6987..7003) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6987..7003) /label=pBluescriptSK /note="For pBluescript vector" polyA_signal 7022..7246 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 11640..12130 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind 12063..12082 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 12157..12726 /codon_start=1 /gene="Corynebacterium diphtheriae tox" /product="diphteria toxin Fragment A" /label=DTA /translation="DDVVILLIFCMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDD DWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELG LSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEIN FETRGKRGQDAMYEYMAQACAGNRVRR" intron 12803..12868 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" polyA_signal 12942..13166 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(13258..13278) /label=T3 /note="T3 promoter, forward primer" promoter complement(13258..13276) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(13297..13313) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(13297..13313) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(13310..13332) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 13321..13337 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(13345..13375) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 13390..13411 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(13528..13545) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(13699..14287) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(13779..13798) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(14458..15318) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 15081..15100 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(15319..15423) /gene="bla" /label=AmpR promoter rep_origin complement(15449..15904) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(15586..15607) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 15798..15817 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 16031..16053 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 16045..16062 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 16046..16062 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 16072..16091 /label=T7 /note="T7 promoter, forward primer" promoter 16072..16090 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 ccccgcggca ggccctccga gcgtggtgga gccgttctgt gagacagccg ggtacgagtc 61 gtgacgctgg aaggggcaag cgggtggtgg gcaggaatgc ggtccgccct gcagcaaccg 121 gagggggagg gagaagggag cggaaaagtc tccaccggac gcggccatgg ctcggggggg 181 ggggggcagc ggaggacgct tccggccgac gtctcgtcgc tgattggctt ttttcctccc 241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg 301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta 361 ggtggggtga ggcgagctga cgtgcgggcg cggtcggcct ctggcggggc gggggagggg 421 agggagggtc agcgaaagta gctcgcgcgc gagcggccgc ccaccctccc cttcctctgg 481 gggagtcgtt ttacccgccg ccggccgggc ctcgtcgtct gattggctct cggggcccag 541 aaaactggcc cttgccattg gctcgtgttc gtgcaagttg agtccatccg ccggccagcg 601 ggggcggcga ggaggcgctc ccaggttccg gccctcccct cggccccgcg ccgcagagtc 661 tggccgcgcg cccctgcgca acgtggcagg aagcgcgcgc tgggggcggg gacgggcagt 721 agggctgagc ggctgcgggg cgggtgcaag cacgtttccg acttgagttg cctcaagagg 781 ggcgtgctga gccagacctc catcgcgcac tccggggagt ggagggaagg agcgagggct 841 cagttgggct gttttggagg caggaagcac ttgctctccc aaagtcgctc tgagttgtta 901 tcagtaaggg agctgcagtg gagtaggcgg ggagaaggcc gcacccttct ccggaggggg 961 gaggggagtg ttgcaatacc tttctgggag ttctctgctg cctcctggct tctgaggacc 1021 gccctgggcc tgggagaatc ccttgccccc tcttcccctc gtgatctgca actccagtct 1081 ttctagcatc tgtagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg 1141 tccctttttt ttccacagct cgcggttgag gacaaactct tcgcggtctt tccagtggtt 1201 aattaagtcg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 1261 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 1321 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 1381 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 1441 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 1501 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1561 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc 1621 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt 1681 gtgcagcgat gggggcgggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg 1741 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa 1801 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg 1861 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg 1921 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc 1981 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg 2041 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc 2101 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga 2161 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc 2221 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg 2281 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct 2341 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg 2401 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg 2461 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc 2521 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag 2581 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg 2641 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg 2701 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg 2761 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg 2821 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca 2881 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcctcga 2941 gttaattaaa taacttcgta tagcatacat tatacgaagt tatggatccg aacaaacgac 3001 ccaacacccg tgcgttttat tctgtctttt tattgccgat cccctcagaa gaactcgtca 3061 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg 3121 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg 3181 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca 3241 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg 3301 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct 3361 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg 3421 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc 3481 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc 3541 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc 3601 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc 3661 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct 3721 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg 3781 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatggcc 3841 gatcccatat tggctgcagg gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg 3901 cgaagtggac ctgggaccgc gccgccccga ctgcatctgc gtgttcgaat tcgccaatga 3961 caagacgctg ggcggggttt gctcgacatt gggtggaaac attccaggcc tgggtggaga 4021 ggctttttgc ttcctcttgc aaaaccacac tgctcgacat tgggtggaaa cattccaggc 4081 ctgggtggag aggctttttg cttcctcttg caaaaccaca ctgctcgact agtgattaaa 4141 gtcgactcgg ggacaccaaa tatggcgatc tcggcctttt cgtttcttgg agctgggaca 4201 tgtttgccat cgatccatct accaccagaa cggccgttag atctgctgcc accgttgttt 4261 ccaccgaaga aaccaccgtt gccgtaacca ccacgacggt tgttgctaaa gaagctgcca 4321 ccgccacggc caccgttgta gccgccgttg ttgttattgt agttgctcat gttatttctg 4381 gcacttcttg gttttcctct taagtgagga ggaacataac cattctcgtt gttgtcgttg 4441 atgcttaaat tttgcacttg ttcgctcagt tcagccataa tatgaaatgc ttttcttgtt 4501 gttcttacgg aataccactt gccacctatc accacaacta actttttccc gttcctccat 4561 ctcttttata ttttttttct cgagggatct ttgtgaagga accttacttc tgtggtgtga 4621 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa 4681 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg 4741 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag 4801 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa 4861 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga 4921 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa 4981 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc 5041 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg 5101 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta 5161 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt 5221 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 5281 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 5341 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 5401 tccaaactca tcaatgtatc ttatcatgtc tggatctgac atggtaagta agcttataac 5461 ttcgtatagc atacattata cgaagttatg ggcgcgccgc ggccgcgatc aattcggtac 5521 cgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc tcgagggtac 5581 cccaattccg cccccccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc 5641 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg 5701 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca 5761 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa 5821 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt 5881 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt 5941 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca 6001 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc 6061 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca 6121 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca 6181 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 6241 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 6301 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 6361 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 6421 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 6481 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 6541 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 6601 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 6661 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 6721 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 6781 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 6841 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa tccgggatcc 6901 ggagagctcc caacgaagtt cctattccga agttcctatt ctctagaaag tataggaact 6961 tcctcgaggt tggatgcagc ccgggggatc cactagttct agagcggccg atcagcctcg 7021 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 7081 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 7141 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 7201 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 7261 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgccgcg 7321 gatatcgaat tcgctagaag atgggcggga gtcttctggg caggcttaaa ggctaacctg 7381 gtgtgtgggc gttgtcctgc aggggaattg aacaggtgta aaattggagg gacaagactt 7441 cccacagatt ttcggttttg tcgggaagtt ttttaatagg ggcaaatagg aaaatggagg 7501 ataggagtca tctggggttt atgcagcaaa actacaggta tattgcttgt atccgcctcg 7561 gagatttcca tgaggagata aagacatgtc acccgagttt atactctcct gcttagatcc 7621 tactacagta tgaaatacag tgtgcgaggt agactatgta agcagattta atcattttaa 7681 agagcccagt acttcatatc catttctccc gctccttctg cagccttatc aaaaggtatt 7741 tagaacactc attttagccc cattttcatt tattatactg gcttatccaa cccctagaca 7801 gagcattggc attttccctt tcctgatctt agaagtctga tgactcatga aaccagacag 7861 attagttaca tacaccacaa atcgaggctg tagctggggc ctcaacactg cagttctttt 7921 ataactcctt agtacacttt ttgttgatcc tttgccttga tccttaattt tcagtgtcta 7981 tcacctctcc cgtcaggtgg tgttccacat ttgggcctat tctcagtcca gggagtttta 8041 caacaataga tgtattgaga atccaaccta aagcttaact ttccactccc atgaatgcct 8101 ctctcctttt tctccattat aactgagcta taccattaat ggtttcaggt ggatgtctcc 8161 tcccccaata tacctgatgt atctacatat tgccaggctg atattttaag acataaaggt 8221 atatttcatt attgagccac atggtattga ttactgctac taaaattttg tcattgtaca 8281 catctgtaaa aggtggttcc ttttggaatg caaagttcag gtgtttgttg tctttcctga 8341 cctaaggtct tgtgagcttg tattttttct atttaagcag tgctttctct tggactggct 8401 tgactcatgg cattctacac gttattgctg gtctaaatgt gattttgcca agcttcttca 8461 ggacctataa ttttgcttga cttgtagcca aacacaagta aaatgattaa gcaacaaatg 8521 tatttgtgaa gcttggtttt taggttgttg tgttgtgtgt gcttgtgctc tataataata 8581 ctatccaggg gctggagagg tggctcggag ttcaagagca cagactgctc ttccagaagt 8641 cctgagttca attcccagca accacatggt ggctcacaac catctgtaat gggatctgat 8701 gccctcttct ggtgtgtctg aagaccacaa gtgtattcac attaaataaa taatcctcct 8761 tcttcttctt tttttttttt taaagagaat ctgtctccag tagaattact gaagtaatga 8821 aatactttgt gtttgttcca atatggagcc aataatcaaa tactcttagc actggaaatg 8881 taccaaggaa ctattttatt taagtgactg tggacagagg agccataact gcagacttgt 8941 gggatacaga agaccaatgc agacttaatg tcttttctct tacactaagc aataaagaaa 9001 taaaaattga acttctagta tcctatttgt taaactgcta gctttactaa cttttgtgct 9061 tcatctatac aaagctgaaa gctaagtctg cagccattac taaacatgaa agcaagtaat 9121 gataattttg gatttcaaaa atgtagggcc agagtttagc cagccagtgg tggtgcttgc 9181 ctttatgcct taatcccagc actctggagg cagagacagg cagatctctg agtttgagcc 9241 cagcctggtc tacacatcaa gttctatcta ggatagccag gaatacacac agaaaccctg 9301 ttggggaggg gggctctgag atttcataaa attataattg aagcattccc taatgagcca 9361 ctatggatgt ggctaaatcc gtctaccttt ctgatgagat ttgggtatta ttttttctgt 9421 ctctgctgtt ggttgggtct tttgacactg tgggctttct taaagcctcc ttccctgcca 9481 tgtggtctct tgtttgctac taacttccca tggcttaaat ggcatggctt tttgccttct 9541 aagggcagct gctgagtttg cagcctgatt tccagggtgg ggttgggaaa tctttcaaac 9601 actaaaattg tcctttaatt tttttttaaa aaatgggtta tataataaac ctcataaaat 9661 agttatgagg agtgaggtgg actaatatta atgagtccct cccctataaa agagctatta 9721 aggctttttg tcttatacta actttttttt taaatgtggt atctttagaa ccaagggtct 9781 tagagtttta gtatacagaa actgttgcat cgcttaatca gattttctag tttcaaatcc 9841 agagaatcca aattcttcac agccaaagtc aaattaagaa tttctgactt taatgttatt 9901 tgctactgtg aatataaaat gatagctttt cctgaggcag ggtctcacta tgtatctctg 9961 cctgatctgc aacaagatat gtagactaaa gttctgcctg cttttgtctc ctgaatacta 10021 aggttaaaat gtagtaatac ttttggaact tgcaggtcag attcttttat aggggacaca 10081 ctaagggagc ttgggtgata gttggtaaat gtgtttaagt gatgaaaact tgaattatta 10141 tcaccgcaac ctacttttta aaaaaaaaag ccaggcctgt tagagcatgc taagggatcc 10201 ctaggacttg ctgagcacac aagagtagta cttggcaggc tcctggtgag agcatatttc 10261 aaaaaacaag gcagacaacc aagaaactac agtaaggtta cctgtcttta accatctgca 10321 tatacacagg gatattaaaa tattccaaat aatatttcat tcaagttttc ccccatcaaa 10381 ttgggacatg gatttctccg gtgaataggc agagttggaa actaaacaaa tgttggtttt 10441 gtgatttgtg aaattgtttt caagtgatag ttaaagccca tgagatacag aacaaagctg 10501 ctatttcgag gtctcttggt tatactcaga agcacttctt tgggtttccc tgcactatcc 10561 tgatcatgtg ctaggcctcc ttaggctgat tgttgttcaa ataacttaag tttcctgtca 10621 ggtgatgtca tatgatttca tatatcaagg caaaacatgt tatatatgtt aaacatttga 10681 cttaatgtga aagttaggtc tttgtgggtt ttgattttaa tttcaaaacc tgagctaaat 10741 aagtcatttt acatgtctta catttggtga attgtatatt gtggtttgca ggcaagactc 10801 tctgacctag taaccctcct atagagcact ttgctgggtc acaagtctag gagtcaagca 10861 tttcaccttg aagttgagac gttttgttag tgtatactag ttatatgttg gaggacatgt 10921 ttatccagaa gatattcagg actatttttg actgggctaa ggaattgatt ctgattagca 10981 ctgttagtga gcattgagtg gcctttaggc ttgaattgga gtcacttgta tatctcaaat 11041 aatgctggcc ttttttaaaa gcccttgttc tttatcaccc tgttttctac ataatttttg 11101 ttcaaagaaa tacttgtttg gatctccttt tgacaacaat agcatgtttt caagccatat 11161 tttttttcct tttttttttt ttttttggtt tttcgagaca gggtttctct gtatagccct 11221 ggctgtcctg gaactcactt tgtagaccag gctggcctcg aactcagaaa tccgcctgcc 11281 tctgcctcct gagtgccggg attaaaggcg tgcaccacca cgcctggcta agttggatat 11341 tttgtatata actataacca atactaactc cactgggtgg atttttaatt cagtcagtag 11401 tcttaagtgg tctttattgg cccttattaa aatctactgt tcactctaac agaggctgtt 11461 ggactagtgg actaagcaac ttcctacgga tatactagca gataagggtc agggatagaa 11521 actagtctag cgttttgtat acctaccagc ttatactacc ttgttctgat agaaatattt 11581 aggacatcta gcttatcgat ccgtcgacgg tatcgataag cttgatatcg aattctaccg 11641 ggtaggggag gcgcttttcc aaggcagtct gagcatgcgc ttagcagccc cgctggcact 11701 tggcgctaca caagtggcct tggcctcgca cacattccac atccaccggt aggcgccaac 11761 cggctccgtt ctttggtggc cccttcgcgc caccttctct cctcccctag tcaggaagtt 11821 cccccccgcc ccgcagctcg cgtcgtagga cgtgacaaat ggaagtagca cgtctcacta 11881 gtctcgtcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg 11941 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg 12001 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc 12061 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc 12121 gggcctttcg acctgcaggt cctcgccatg gatcctgatg atgttgttat tcttctaatc 12181 ttttgtatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt 12241 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa 12301 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgtaga taatgaaaac 12361 ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt atccaggact gacgaaggtt 12421 ctcgcactaa aagtggataa tgccgaaact attaagaaag agttaggttt aagtctcact 12481 gaaccgttga tggagcaagt cggaacggaa gagtttatca aaaggttcgg tgatggtgct 12541 tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt ctagcgttga atatattaat 12601 aactgggaac aggcgaaagc gttaagcgta gaacttgaga ttaattttga aacccgtgga 12661 aaacgtggcc aagatgcgat gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc 12721 aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc 12781 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact 12841 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca 12901 gtggtggaat gcagatccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca 12961 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 13021 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 13081 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 13141 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgac 13201 ctcgaggggg ggcccgcggc cgccctgcag ggcgatcgct acgtacagct tttgttccct 13261 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 13321 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 13381 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 13441 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 13501 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 13561 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 13621 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 13681 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 13741 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 13801 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 13861 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 13921 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 13981 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 14041 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 14101 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 14161 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 14221 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 14281 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 14341 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 14401 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 14461 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 14521 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 14581 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 14641 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 14701 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 14761 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 14821 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 14881 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 14941 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 15001 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 15061 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 15121 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 15181 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 15241 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 15301 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 15361 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 15421 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc 15481 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc 15541 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag 15601 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc 15661 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa 15721 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg 15781 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt 15841 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc 15901 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct 15961 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg 16021 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac 16081 tcactatagg gcgaattgga gct //