LOCUS       Exported                4179 bp ds-DNA     linear   SYN 14-JUN-2021
DEFINITION  Localization: Desmosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    mEmerald-Desmoglein2-C-18
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4179)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      23..32
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             29..745
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(74..95)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(335..354)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     682..703
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccggcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
       61 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      121 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      181 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg
      241 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      301 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      361 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      421 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt
      481 ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa
      541 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      601 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
      661 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      721 tctcggcatg gacgagctgt acaagtccgg aagtggagct gggagcgggg caagcggaag
      781 cggaagtggc tccggtgggg cgcggagccc gggacgcgcg tacgccctgc tgcttctcct
      841 gatctgcttt aacgttggaa gtggacttca cttacaggtc ttaagcacaa gaaatgaaaa
      901 taagctgctt cctaaacatc ctcatttagt gcggcaaaag cgcgcctgga tcaccgcccc
      961 cgtggctctt cgggagggag aggatctgtc caagaagaat ccaattgcca agatacattc
     1021 tgatcttgca gaagaaagag gactcaaaat tacttacaaa tacactggaa aagggattac
     1081 agagccacct tttggtatat ttgtctttaa caaagatact ggagaactga atgttaccag
     1141 cattcttgat cgagaagaaa caccattttt tctgctaaca ggttacgctt tggatgcaag
     1201 aggaaacaat gtagagaaac ccttagagct acgcattaag gttcttgata tcaatgacaa
     1261 cgaaccagtg ttcacacagg atgtctttgt tgggtctgtt gaagagttga gtgcagcaca
     1321 tactcttgtg atgaaaatca atgcaacaga tgcagatgag cccaataccc tgaactcgaa
     1381 aatttcctat agaatcgtat ctctggagcc tgcttatcct ccagtgttct acctaaataa
     1441 agatacagga gagatttata caaccagtgt taccttggac agagaggaac acagcagcta
     1501 cactttgaca gtagaagcaa gagatggcaa tggagaagtt acagacaaac ctgtaaaaca
     1561 agctcaagtt cagattcgta ttttggatgt caatgacaat atacctgtag tagaaaataa
     1621 agtgcttgaa gggatggttg aagaaaatca agtcaacgta gaagttacgc gcataaaagt
     1681 gttcgatgca gatgaaatag gttctgataa ttggctggca aattttacat ttgcatcagg
     1741 aaatgaagga ggttatttcc acatagaaac agatgctcaa actaacgaag gaattgtgac
     1801 ccttattaag gaagtagatt atgaagaaat gaagaatctt gacttcagtg ttattgtcgc
     1861 taataaagca gcttttcaca agtcgattag gagtaaatac aagcctacac ccattcccat
     1921 caaggtcaaa gtgaaaaatg tgaaagaagg cattcatttt aaaagcagcg tcatctcaat
     1981 ttatgttagc gagagcatgg atagatcaag caaaggccaa ataattggaa attttcaagc
     2041 atttgatgag gacactggac taccagccca tgcaagatat gtaaaattag aagatagaga
     2101 taattggatc tctgtggatt ctgtcacatc tgaaattaaa cttgcaaaac ttcctgattt
     2161 tgaatctaga tatgttcaaa atggcacata cactgtaaag attgtggcca tatcagaaga
     2221 ttatcctaga aaaaccatca ctggcacagt ccttatcaat gttgaagaca tcaacgacaa
     2281 ctgtcccaca ctgatagagc ctgtgcagac aatctgtcac gatgcagagt atgtgaatgt
     2341 tactgcagag gacctggatg gacacccaaa cagtggccct ttcagtttct ccgtcattga
     2401 caaaccacct ggcatggcag aaaaatggaa aatagcacgc caagaaagta ccagtgtgct
     2461 gctgcaacaa agtgagaaaa agctagggag aagtgaaatt cagttcctga tttcagacaa
     2521 tcagggtttt agttgtcctg aaaagcaggt ccttacactc acagtttgtg agtgtctgca
     2581 tggcagcggc tgcagggaag cacagcatga ctcctatgtg ggcctgggac ccgcagcaat
     2641 tgcgctcatg attttggcct ttctgctcct gctattggtg ccacttttac tgctgatgtg
     2701 ccattgcgga aagggcgcca aaggctttac ccccatacct ggcaccatag agatgctgca
     2761 tccttggaat aatgaaggag caccacctga agacaaggtg gtgccatcat ttctgccagt
     2821 ggatcaaggg ggcagtctag taggaagaaa tggagtagga ggtatggcca aggaagccac
     2881 gatgaaagga agtagctctg cttccattgt caaagggcaa catgagatgt ccgagatgga
     2941 tggaaggtgg gaagaacaca gaagcctgct ttctggtaga gctacccagt ttacaggggc
     3001 cacaggcgct atcatgacca ctgaaaccac gaagaccgca agggccacag gggcttccag
     3061 agacatggcc ggagctcagg cagctgctgt tgcactgaac gaagagttct taagaaatta
     3121 tttcactgat aaagcggcct cttacactga ggaagatgaa aatcacacag ccaaagattg
     3181 ccttctggtt tattctcagg aagaaactga atcgctgaat gcttctattg gttgttgcag
     3241 ttttattgaa ggagagctag atgaccgctt cttagatgat ttgggactta aattcaagac
     3301 actagctgaa gtttgcctgg gtcaaaaaat agatataaat aaggaaattg agcagagaca
     3361 aaaacctgcc acagaaacaa gtatgaacac agcttcacat tcactctgtg agcaaactat
     3421 ggttaattca gagaatacct actcctctgg cagtagcttc ccagttccaa aatctttgca
     3481 agaagccaat gcagagaaag taactcagga aatagtcact gaaaggtctg tgtcttctag
     3541 gcaggcgcaa aaggtagcta cacctcttcc tgacccaatg gcttctagaa atgtgatagc
     3601 aacagaaact tcctatgtca cagggtccac tatgccacca accactgtga tcctgggtcc
     3661 tagccagcca cagagcctta ttgtgacaga gagggtgtat gctccagctt ctaccttggt
     3721 agatcagcct tatgctaatg aaggtacagt tgtggtcact gaaagagtaa tacagcctca
     3781 tgggggtgga tcgaatcctc tggaaggcac tcagcatctt caagatgtac cttacgtcat
     3841 ggtgagggaa agagagagct tccttgcccc cagctcaggt gtgcagccta ctctggccat
     3901 gcctaatata gcagtaggac agaatgtgac agtgacagaa agagttctag cacctgcttc
     3961 cactctgcaa tccagttacc agattcccac tgaaaattct atgacggcta ggaacaccac
     4021 ggtgtctgga gctggagtcc ctggccctct gccagatttt ggtttagagg aatctggtca
     4081 ttctaattct accataacca catcttccac cagagttacc aagcatagca ctgtacagca
     4141 ttcttactcc taaggatcca ccggatctag ataactgat
//
