LOCUS Exported 8727 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-FilaminA-N-9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8727) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 8727) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8727 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 7964..7973 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 7970..8689 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(8015..8036) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(8276..8295) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 8623..8644 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 tgacgtcaga tccgctagcg ccaccatgag tagctcccac tctcgggcgg gccagagcgc 61 agcaggcgcg gctccgggcg gcggcgtcga cacgcgggac gccgagatgc cggccaccga 121 gaaggacctg gcggaggacg cgccatggaa gaagatccag cagaacactt tcacgcgctg 181 gtgcaacgag cacctgaagt gcgtgagcaa gcgcatcgcc aacctgcaga cggacctgag 241 cgacgggctg cggcttatcg cgctgttgga ggtgctcagc cagaagaaga tgcaccgcaa 301 gcacaaccag cggcccactt tccgccaaat gcagcttgag aacgtgtcgg tggcactcga 361 gttcctggac cgcgagagca tcaaactggt gtccatcgac agcaaggcca tcgtggacgg 421 gaacctgaag ctgatcctgg gcctcatctg gaccctgatc ctgcactact ccatctccat 481 gcccatgtgg gacgaggagg aggatgagga ggccaagaag cagaccccca agcagaggct 541 cctgggctgg atccagaaca agctgccgca gctgcccatc accaacttca gccgggactg 601 gcagagcggc cgggccctgg gcgccctggt ggacagctgt gccccgggcc tgtgtcctga 661 ctgggactct tgggacgcca gcaagcccgt taccaatgcg cgagaggcca tgcagcaggc 721 ggatgactgg ctgggcatcc cccaggtgat cacccccgag gagattgtgg accccaacgt 781 ggacgagcac tctgtcatga cctacctgtc ccagttcccc aaggccaagc tgaagccagg 841 ggctcccttg cggcccaaac tgaacccgaa gaaagcccgt gcctacgggc caggcatcga 901 gcccacaggc aacatggtga agaagcgggc agagttcact gtggagacca gaagtgctgg 961 ccagggagag gtgctggtgt acgtggagga cccggccgga caccaggagg aggcaaaagt 1021 gaccgccaat aacgacaaga accgcacctt ctccgtctgg tacgtccccg aggtgacggg 1081 gactcataag gttactgtgc tctttgctgg ccagcacatc gccaagagcc ccttcgaggt 1141 gtacgtggat aagtcacagg gtgacgccag caaagtgaca gcccaaggtc ccggcctgga 1201 gcccagtggc aacatcgcca acaagaccac ctactttgag atctttacgg caggagctgg 1261 cacgggcgag gtcgaggttg tgatccagga ccccatggga cagaagggca cggtagagcc 1321 tcagctggag gcccggggcg acagcacata ccgctgcagc taccagccca ccatggaggg 1381 cgtccacacc gtgcacgtca cgtttgccgg cgtgcccatc cctcgcagcc cctacactgt 1441 cactgttggc caagcctgta acccgagtgc ctgccgggcg gttggccggg gcctccagcc 1501 caagggtgtg cgggtgaagg agacagctga cttcaaggtg tacacaaagg gcgctggcag 1561 tggggagctg aaggtcaccg tgaagggccc caagggagag gagcgcgtga agcagaagga 1621 cctgggggat ggcgtgtatg gcttcgagta ttaccccatg gtccctggaa cctatatcgt 1681 caccatcacg tggggtggtc agaacatcgg gcgcagtccc ttcgaagtga aggtgggcac 1741 cgagtgtggc aatcagaagg tacgggcctg gggccctggg ctggagggcg gcgtcgttgg 1801 caagtcagca gactttgtgg tggaggctat cggggacgac gtgggcacgc tgggcttctc 1861 ggtggaaggg ccatcgcagg ctaagatcga atgtgacgac aagggcgacg gctcctgtga 1921 tgtgcgctac tggccgcagg aggctggcga gtatgccgtt cacgtgctgt gcaacagcga 1981 agacatccgc ctcagcccct tcatggctga catccgtgac gcgccccagg acttccaccc 2041 agacagggtg aaggcacgtg ggcctggatt ggagaagaca ggtgtggccg tcaacaagcc 2101 agcagagttc acagtggatg ccaagcacgg tggcaaggcc ccacttcggg tccaagtcca 2161 ggacaatgaa ggctgccctg tggaggcgtt ggtcaaggac aacggcaatg gcacttacag 2221 ctgctcctac gtgcccagga agccggtgaa gcacacagcc atggtgtcct ggggaggcgt 2281 cagcatcccc aacagcccct tcagggtgaa tgtgggagct ggcagccacc ccaacaaggt 2341 caaagtatac ggccccggag tagccaagac agggctcaag gcccacgagc ccacctactt 2401 cactgtggac tgcgccgagg ctggccaggg ggacgtcagc atcggcatca agtgtgcccc 2461 tggagtggta ggccccgccg aagctgacat cgacttcgac atcatccgca atgacaatga 2521 caccttcacg gtcaagtaca cgccccgggg ggctggcagc tacaccatta tggtcctctt 2581 tgctgaccag gccacgccca ccagccccat ccgagtcaag gtggagccct ctcatgacgc 2641 cagtaaggtg aaggccgagg gccctggcct cagtcgcact ggtgtcgagc ttggcaagcc 2701 cacccacttc acagtaaatg ccaaagctgc tggcaaaggc aagctggacg tccagttctc 2761 aggactcacc aagggggatg cagtgcgaga tgtggacatc atcgaccacc atgacaacac 2821 ctacacagtc aagtacacgc ctgtccagca gggtccagta ggcgtcaatg tcacttatgg 2881 aggggatccc atccctaaga gccctttctc agtggcagta tctccaagcc tggacctcag 2941 caagatcaag gtgtctggcc tgggagagaa ggtggacgtt ggcaaagacc aggagttcac 3001 agtcaaatca aagggtgctg gtggtcaagg caaagtggca tccaagattg tgggcccctc 3061 gggtgcagcg gtgccctgca aggtggagcc aggcctgggg gctgacaaca gtgtggtgcg 3121 cttcctgccc cgtgaggaag ggccctatga ggtggaggtg acctatgacg gcgtgcccgt 3181 gcctggcagc ccctttcctc tggaagctgt ggcccccacc aagcctagca aggtgaaggc 3241 gtttgggccg gggctgcagg gaggcagtgc gggctccccc gcccgcttca ccatcgacac 3301 caagggcgcc ggcacaggtg gcctgggcct gacggtggag ggcccctgtg aggcgcagct 3361 cgagtgcttg gacaatgggg atggcacatg ttccgtgtcc tacgtgccca ccgagcccgg 3421 ggactacaac atcaacatcc tcttcgctga cacccacatc cctggctccc cattcaaggc 3481 ccacgtggtt ccctgctttg acgcatccaa agtcaagtgc tcaggccccg ggctggagcg 3541 ggccaccgct ggggaggtgg gccaattcca agtggactgc tcgagcgcgg gcagcgcgga 3601 gctgaccatt gagatctgct cggaggcggg gcttccggcc gaggtgtaca tccaggacca 3661 cggtgatggc acgcacacca ttacctacat tcccctctgc cccggggcct acaccgtcac 3721 catcaagtac ggcggccagc ccgtgcccaa cttccccagc aagctgcagg tggaacctgc 3781 ggtggacact tccggtgtcc agtgctatgg gcctggtatt gagggccagg gtgtcttccg 3841 tgaggccacc actgagttca gtgtggacgc ccgggctctg acacagaccg gagggccgca 3901 cgtcaaggcc cgtgtggcca acccctcagg caacctgacg gagacctacg ttcaggaccg 3961 tggcgatggc atgtacaaag tggagtacac gccttacgag gagggactgc actccgtgga 4021 cgtgacctat gacggcagtc ccgtgcccag cagccccttc caggtgcccg tgaccgaggg 4081 ctgcgacccc tcccgggtgc gtgtccacgg gccaggcatc caaagtggca ccaccaacaa 4141 gcccaacaag ttcactgtgg agaccagggg agctggcacg ggcggcctgg gcctggctgt 4201 agagggcccc tccgaggcca agatgtcctg catggataac aaggacggca gctgctcggt 4261 cgagtacatc ccttatgagg ctggcaccta cagcctcaac gtcacctatg gtggccatca 4321 agtgccaggc agtcctttca aggtccctgt gcatgatgtg acagatgcgt ccaaggtcaa 4381 gtgctctggg cccggcctga gcccaggcat ggttcgtgcc aacctccctc agtccttcca 4441 ggtggacaca agcaaggctg gtgtggcccc attgcaggtc aaagtgcaag ggcccaaagg 4501 cctggtggag ccagtggacg tggtagacaa cgctgatggc acccagaccg tcaattatgt 4561 gcccagccga gaagggccct acagcatctc agtactgtat ggagatgaag aggtaccccg 4621 gagccccttc aaggtcaagg tgctgcctac tcatgatgcc agcaaggtga aggccagtgg 4681 ccccgggctc aacaccactg gcgtgcctgc cagcctgccc gtggagttca ccatcgatgc 4741 aaaggacgcc ggggagggcc tgctggctgt ccagatcacg gatcccgaag gcaagccgaa 4801 gaagacacac atccaagaca accatgacgg cacgtataca gtggcctacg tgccagacgt 4861 gacaggtcgc tacaccatcc tcatcaagta cggtggtgac gagatcccct tctccccgta 4921 ccgcgtgcgt gccgtgccca ccggggacgc cagcaagtgc actgtcacag gtgctggcat 4981 cggccccacc attcagattg gggaggagac ggtgatcact gtggacacta aggcggcagg 5041 caaaggcaaa gtgacgtgca ccgtgtgcac gcctgatggc tcagaggtgg atgtggacgt 5101 ggtggagaat gaggacggca ctttcgacat cttctacacg gccccccagc cgggcaaata 5161 cgtcatctgt gtgcgctttg gtggcgagca cgtgcccaac agccccttcc aagtgacggc 5221 tctggctggg gaccagccct cggtgcagcc ccctctacgg tctcagcagc tggccccaca 5281 gtacacctac gcccagggcg gccagcagac ttgggccccg gagaggcccc tggtgggtgt 5341 caatgggctg gatgtgacca gcctgaggcc ctttgacctt gtcatcccct tcaccatcaa 5401 gaagggcgag atcacagggg aggttcggat gccctcaggc aaggtggcgc agcccaccat 5461 cactgacaac aaagacggca ccgtgaccgt gcggtatgca cccagcgagg ctggcctgca 5521 cgagatggac atccgctatg acaacatgca catcccagga agccccttgc agttctatgt 5581 ggattacgtc aactgtggcc atgtcactgc ctatgggcct ggcctcaccc atggagtagt 5641 gaacaagcct gccaccttca ccgtcaacac caaggatgca ggagaggggg gcctgtctct 5701 ggccattgag ggcccgtcca aagcagaaat cagctgcact gacaaccagg atgggacatg 5761 cagcgtgtcc tacctgcctg tgctgccggg ggactacagc attctagtca agtacaatga 5821 acagcacgtc ccaggcagcc ccttcactgc ccgggtcaca ggtgacgact ccatgcgtat 5881 gtcccaccta aaggtcggct ctgctgccga catccccatc aacatctcag agacggatct 5941 cagcctgctg acggccactg tggtcccgcc ctcgggccgg gaggagccct gtttgctgaa 6001 gcggctgcgt aatggccacg tggggatttc attcgtgccc aaggagacgg gggagcacct 6061 ggtgcatgtg aagaaaaatg gccagcacgt ggccagcagc cccatcccgg tggtgatcag 6121 ccagtcggaa attggggatg ccagtcgtgt tcgggtctct ggtcagggcc ttcacgaagg 6181 ccacaccttt gagcctgcag agtttatcat tgatacccgc gatgcaggct atggtgggct 6241 cagcctgtcc attgagggcc ccagcaaggt ggacatcaac acagaggacc tggaggacgg 6301 gacgtgcagg gtcacctact gccccacaga gccaggcaac tacatcatca acatcaagtt 6361 tgccgaccag cacgtgcctg gcagcccctt ctctgtgaag gtgacaggcg agggccgggt 6421 gaaagagagc atcacccgca ggcgtcgggc tccttcagtg gccaacgttg gtagtcattg 6481 tgacctcagc ctgaaaatcc ctgaaattag catccaggat atgacagccc aggtgaccag 6541 cccatcgggc aagacccatg aggccgagat cgtggaaggg gagaaccaca cctactgcat 6601 ccgctttgtt cccgctgaga tgggcacaca cacagtcagc gtcaagtaca agggccagca 6661 cgtgcctggg agccccttcc agttcaccgt ggggccccta ggggaagggg gagcccacaa 6721 ggtccgagct gggggccctg gcctggagag agctgaagct ggagtgccag ccgaattcag 6781 tatctggacc cgggaagctg gtgctggagg cctggccatt gctgtcgagg gccccagcaa 6841 ggctgagatc tcttttgagg accgcaagga cggctcctgt ggtgtggctt atgtggtcca 6901 ggagccaggt gactacgaag tctcagtcaa gttcaacgag gaacacattc ccgacagccc 6961 cttcgtggtg cctgtggctt ctccgtctgg cgacgcccgc cgcctcactg tttctagcct 7021 tcaggagtca gggctaaagg tcaaccagcc agcctctttt gcagtcagcc tgaacggggc 7081 caagggggcg atcgatgcca aggtgcacag cccctcagga gccctggagg agtgctatgt 7141 cacagaaatt gaccaagata agtatgctgt gcgcttcatc cctcgggaga atggcgttta 7201 cctgattgac gtcaagttca acggtaccca catccctgga agccccttca agatccgagt 7261 tggggagcct gggcatggag gggacccagg cttggtgtct gcttacggag caggtctgga 7321 aggcggtgtc acagggaacc cagctgagtt cgtcgtgaac acgagcaatg cgggagctgg 7381 tgccctgtcg gtgaccattg acggcccctc caaggtgaag atggattgcc aggagtgccc 7441 tgagggctac cgcgtcacct atacccccat ggcacctggc agctacctca tctccatcaa 7501 gtacggcggc ccctaccaca ttgggggcag ccccttcaag gccaaagtca caggcccccg 7561 tctcgtcagc aaccacagcc tccacgagac atcatcagtg tttgtagact ctctgaccaa 7621 ggccacctgt gccccccagc atggggcccc gggtcctggg cctgctgacg ccagcaaggt 7681 ggtggccaag ggcctggggc tgagcaaggc ctacgtaggc cagaagagca gcttcacagt 7741 agactgcagc aaagcaggca acaacatgct gctggtgggg gttcatggcc caaggacccc 7801 ctgcgaggag atcctggtga agcacgtggg cagccggctc tacagcgtgt cctacctgct 7861 caaggacaag ggggagtaca cactggtggt caaatggggg cacgagcaca tcccaggcag 7921 cccctaccgc gttgtggtgc ccagcggcag cgcctcaccg gtcgccacca tggtgagcaa 7981 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 8041 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 8101 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 8161 cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt 8221 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 8281 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 8341 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 8401 caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt 8461 gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 8521 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac 8581 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 8641 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag cggccgcgac 8701 tctagatcat aatcagccat accacat //