LOCUS       Exported                3218 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Localization: Integrin/Focal Adhesions, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    mEmerald-Integrin-Beta3-N-18
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3218)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      2455..2464
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2461..3180
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2506..2527)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2767..2786)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3114..3135
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgaga ccgccatgcg agcgcagtgg
       61 ccgggacaac tctgggccgc tctgctggcg ctgggggcgc tggcgggcgt tgttgttgga
      121 gagtccaaca tctgtaccac acgaggcgtg aactcctgcc agcagtgtct ggctgtgagt
      181 cctgtgtgtg cctggtgctc agatgagact ttgtctcagg gctcaccccg atgtaacctg
      241 aaggagaacc tgctgaagga caattgtgct ccagagtcta ttgagttccc agtcagtgag
      301 gcccagatcc tggaggctag gccactcagc agcaagggct ctggaagcag cgcccagatc
      361 actcaagtca gccctcagag gattgccctt cgactacggc cagatgattc gaagatcttc
      421 tcacttcaag tgcggcaggt ggaggattac cccgtggaca tctactactt gatggacctg
      481 tctttctcca tgaaggatga tctgtccagc atccagaccc tgggtaccaa gttggcctct
      541 cagatgcgca agcttactag caaccttcgg atcggctttg gggccttcgt ggacaagcct
      601 gtatcgccgt acatgtacat ctccccacca caggcaatca aaaacccctg ttacaatatg
      661 aagaatgcct gcttgcccat gtttggctac aaacacgtgc tgacgctaac cgaccaggtg
      721 tcccgcttca atgaagaagt gaagaaacag agcgtgtccc gtaatcgaga tgccccagag
      781 ggcggctttg acgccatcat gcaggctaca gtatgtgatg aaaaaattgg ctggaggaat
      841 gacgcatccc atttgctagt gtttaccacg gatgccaaga cccatattgc cctggatgga
      901 aggctggcag gcattgtcct gcccaatgat gggcactgtc acattggcac cgacaaccac
      961 tactctgcct ccactaccat ggactaccca tctctggggc tgatgactga gaaactatcc
     1021 cagaaaaaca ttaacttgat ctttgcagtg actgaaaatg tcgtcagcct ttaccagaat
     1081 tatagtgagc tcattcctgg gaccacagtg ggagtcctgt ctgatgactc aagcaacgtc
     1141 ctccagctca ttgttgatgc ttacgggaaa atccgctcta aagtggagct ggaagtacgt
     1201 gacctgccgg aagaactgtc actgtccttc aatgccacct gcctcaacaa cgaggttatc
     1261 ccgggcctca agtcttgtgt gggcctcaag attggagaca cggtgagctt tagtatcgag
     1321 gccaaggtgc gtggctgccc ccaggagaag gagcagtctt tcactatcaa gcctgtgggc
     1381 tttaaggaca gcctcaccgt ccaggtgacc ttcgactgtg actgtgcctg ccaggccttt
     1441 gcccagcctt ccagcccacg ctgcaacaat gggaacggga cttttgagtg tggggtgtgc
     1501 cgctgtgacc agggctggct ggggtccatg tgtgagtgct ctgaggagga ttaccgaccc
     1561 tctcagcagg aagagtgcag ccccaaggag ggccagccca tctgcagcca gcggggagag
     1621 tgcctctgtg gccagtgtgt ctgccatagc agcgacttcg gcaagatcac tggcaagtac
     1681 tgtgagtgcg atgacttctc ctgcgtccgc tacaaagggg agatgtgttc cggccatggg
     1741 caatgtaact gtggggactg cgtgtgtgac tcggactgga ctggctacta ctgcaactgt
     1801 actacacgca ctgacacctg catgtccacc aatgggctgc tgtgcagcgg ccggggcaac
     1861 tgcgagtgcg gcagctgtgt gtgcgtccag ccaggctcct atggagacac ctgtgagaag
     1921 tgccccacct gcccagatgc ctgctccttt aagaaggagt gtgtggagtg taagaagttc
     1981 aaccggggaa cgctccatga agaaaacacc tgcagccgct actgccggga cgacatcgag
     2041 caggtgaaag agctgacgga tactggcaaa aacgccgtga attgtaccta caagaacgag
     2101 gatgactgtg tcgtcagatt ccagtactac gaagacacca gtgggagggc agtcctctat
     2161 gtggtggaag agcctgagtg tcccaagggt cctgatatcc tggtggtcct gctgtcagtg
     2221 atgggggcca tcctgctcat tggccttgct actctgctca tctggaagct actcatcacc
     2281 atccatgacc ggaaggaatt tgctaaattt gaggaagaac gagccagagc caagtgggac
     2341 acagcaaaca acccgctgta taaagaggcc acctccacct tcaccaatat cacctaccgg
     2401 gggactggta gcgcatccgg gggtgcgtca ggcggcagcg gatctggacc ggtcgccacc
     2461 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2521 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     2581 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2641 ctcgtgacca ccttgaccta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     2701 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     2761 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2821 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2881 aagctggagt acaactacaa cagccacaag gtctatatca ccgccgacaa gcagaagaac
     2941 ggcatcaagg tgaacttcaa gacccgccac aacatcgagg acggcagcgt gcagctcgcc
     3001 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     3061 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3121 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     3181 gcggccgcga ctctagatca taatcagcca taccacat
//
