LOCUS Exported 2808 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Localization: Nuclear Envelope, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-LaminA-C-18 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2808) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 2808) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2808 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 21..30 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 27..743 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(72..93) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(333..352) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 680..701 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg 61 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 121 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 181 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct 241 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 301 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 361 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 421 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct 481 atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca 541 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 601 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc 661 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 721 tcggcatgga cgagctgtac aagtccggac tcagatctgg cagcggtgga ggcagcgcat 781 ccggcggaag cggaagcgag accccgtccc agcggcgcgc cacccgcagc ggggcgcagg 841 ccagctccac tccgctgtcg cccacccgca tcacccggct gcaggagaag gaggacctgc 901 aggagctcaa tgatcgcttg gcggtctaca tcgaccgtgt gcgctcgctg gaaacggaga 961 acgcagggct gcgccttcgc atcaccgagt ctgaagaggt ggtcagccgc gaggtgtccg 1021 gcatcaaggc cgcctacgag gccgagctcg gggatgcccg caagaccctt gactcagtag 1081 ccaaggagcg cgcccgcctg cagctggagc tgagcaaagt gcgtgaggag tttaaggagc 1141 tgaaagcgcg caataccaag aaggagggtg acctgatagc tgctcaggct cggctgaagg 1201 acctggaggc tctgctgaac tccaaggagg ccgcactgag cactgctctc agtgagaagc 1261 gcacgctgga gggcgagctg catgatctgc ggggccaggt ggccaagctt gaggcagccc 1321 taggtgaggc caagaagcaa cttcaggatg agatgctgcg gcgggtggat gctgagaaca 1381 ggctgcagac catgaaggag gaactggact tccagaagaa catctacagt gaggagctgc 1441 gtgagaccaa gcgccgtcat gagacccgac tggtggagat tgacaatggg aagcagcgtg 1501 agtttgagag ccggctggcg gatgcgctgc aggaactgcg ggcccagcat gaggaccagg 1561 tggagcagta taagaaggag ctggagaaga cttattctgc caagctggac aatgccaggc 1621 agtctgctga gaggaacagc aacctggtgg gggctgccca cgaggagctg cagcagtcgc 1681 gcatccgcat cgacagcctc tctgcccagc tcagccagct ccagaagcag ctggcagcca 1741 aggaggcgaa gcttcgagac ctggaggact cactggcccg tgagcgggac accagccggc 1801 ggctgctggc ggaaaaggag cgggagatgg ccgagatgcg ggcaaggatg cagcagcagc 1861 tggacgagta ccaggagctt ctggacatca agctggccct ggacatggag atccacgcct 1921 accgcaagct cttggagggc gaggaggaga ggctacgcct gtcccccagc cctacctcgc 1981 agcgcagccg tggccgtgct tcctctcact catcccagac acagggtggg ggcagcgtca 2041 ccaaaaagcg caaactggag tccactgaga gccgcagcag cttctcacag cacgcacgca 2101 ctagcgggcg cgtggccgtg gaggaggtgg atgaggaggg caagtttgtc cggctgcgca 2161 acaagtccaa tgaggaccag tccatgggca attggcagat caagcgccag aatggagatg 2221 atcccttgct gacttaccgg ttcccaccaa agttcaccct gaaggctggg caggtggtga 2281 cgatctgggc tgcaggagct ggggccaccc acagcccccc taccgacctg gtgtggaagg 2341 cacagaacac ctggggctgc gggaacagcc tgcgtacggc tctcatcaac tccactgggg 2401 aagaagtggc catgcgcaag ctggtgcgct cagtgactgt ggttgaggac gacgaggatg 2461 aggatggaga tgacctgctc catcaccacc acggctccca ctgcagcagc tcgggggacc 2521 ccgctgagta caacctgcgc tcgcgcaccg tgctgtgcgg gacctgcggg cagcctgccg 2581 acaaggcatc tgccagcggc tcaggagccc aggtgggcgg acccatctcc tctggctctt 2641 ctgcctccag tgtcacggtc actcgcagct accgcagtgt ggggggcagt gggggtggca 2701 gcttcgggga caatctggtc acccgctcct acctcctggg caactccagc ccccgaaccc 2761 agagccccca gaactgcagc atcatgtaag gatccaccgg atctagat //