LOCUS Exported 2556 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION Localization: Nuclear Envelope, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-LaminB1-10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2556) AUTHORS Day RN, Davidson MW TITLE The fluorescent protein palette: tools for cellular imaging. JOURNAL Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 2009 Aug 4. PUBMED 19771335 REFERENCE 2 (bases 1 to 2556) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2556 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 26..35 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 32..748 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(77..98) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(338..357) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 685..706 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac 61 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca 241 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 481 ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca 541 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa 661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 721 cactctcggc atggacgagc tgtacaagtc cggactcaga tctcgagctc aagcttcgat 781 ggcgactgcg acccccgtgc cgccgcggat gggcagccgc gctggcggcc ccaccacgcc 841 gctgagcccc acgcgcctgt cgcggctcca ggagaaggag gagctgcgcg agctcaatga 901 ccggctggcg gtgtacatcg acaaggtgcg cagcctggag acggagaaca gcgcgctgca 961 gctgcaggtg acggagcgcg aggaggtgcg cggccgtgag ctcaccggcc tcaaggcgct 1021 ctacgagacc gagctggccg acgcgcgacg cgcgctcgac gacacggccc gcgagcgcgc 1081 caagctgcag atcgagctgg gcaagtgcaa ggcggaacac gaccagctgc tcctcaacta 1141 tgctaagaag gaatctgatc ttaatggcgc ccagatcaag cttcgagaat atgaagcagc 1201 actcaattcg aaagatgcag ctcttgctac tgcacttggt gacaaaaaaa gtttagaggg 1261 agatttggag gatctgaagg atcagattgc ccagttggaa gcctccttag ctgcagccaa 1321 aaaacagtta gcagatgaaa ctttacttaa agtagatttg gagaatcgtt gtcagagcct 1381 tactgaggac ttggagtttc gcaaaagcat gtatgaagag gagattaacg agaccagaag 1441 gaagcatgaa acgcgcttgg tagaggtgga ttctgggcgt caaattgagt atgagtacaa 1501 gctggcgcaa gcccttcatg agatgagaga gcaacatgat gcccaagtga ggctgtataa 1561 ggaggagctg gagcagactt accatgccaa acttgagaat gccagactgt catcagagat 1621 gaatacttct actgtcaaca gtgccaggga agaactgatg gaaagccgca tgagaattga 1681 gagcctttca tcccagcttt ctaatctaca gaaagagtct agagcatgtt tggaaaggat 1741 tcaagaatta gaggacttgc ttgctaaaga aaaagacaac tctcgtcgca tgctgacaga 1801 caaagagaga gagatggcgg aaataaggga tcaaatgcag caacagctga atgactatga 1861 acagcttctt gatgtaaagt tagccctgga catggaaatc agtgcttaca ggaaactctt 1921 agaaggcgaa gaagagaggt tgaagctgtc tccaagccct tcttcccgtg tgacagtatc 1981 ccgagcatcc tcaagtcgta gtgtacgtac aactagagga aagcggaaga gggttgatgt 2041 ggaagaatca gaggcgagta gtagtgttag catctctcat tccgcctcag ccactggaaa 2101 tgtttgcatc gaagaaattg atgttgatgg gaaatttatc cgcttgaaga acacttctga 2161 acaggatcaa ccaatgggag gctgggagat gatcagaaaa attggagaca catcagtcag 2221 ttataaatat acctcaagat atgtgctgaa ggcaggccag actgttacaa tttgggctgc 2281 aaacgctggt gtcacagcca gccccccaac tgacctcatc tggaagaacc agaactcgtg 2341 gggcactggc gaagatgtga aggttatatt gaaaaattct cagggagagg aggttgctca 2401 aagaagtaca gtctttaaaa caaccatacc tgaagaagag gaggaggagg aagaagcagc 2461 tggagtggtt gttgaggaag aacttttcca ccagcaggga accccaagag catccaatag 2521 aagctgtgca attatgtagg gatccaccgg atctag //