LOCUS Exported 1339 bp ds-DNA linear SYN 12-JUN-2021 DEFINITION Localization: Light Chain Myosin, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-LC-Myosin-N-7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1339) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 1339) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1339 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 575..584 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 581..1300 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(626..647) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(887..906) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1234..1255 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 agatccgcta gcgctaccgg actcagatct cgagctcaag cttatgtcga gcaagagagc 61 caaggccaag accaccaaga agaggcccca gagggctacg tccaatgtct tcgccatgtt 121 tgaccagtcc cagatccagg agtttaagga ggccttcaac atgattgatc agaaccgcga 181 tggcttcatt gataaggagg acctgcacga catgttggcc tctctgggga agaaccccac 241 agacgagtac ctggagggca tgatgaacga ggcgccaggg cccatcaact tcaccatgtt 301 cctcacaatg tttggggaga agctgaacgg cacagacccc gaggatgtga tccgcaatgc 361 ctttgcctgc tttgatgagg aggcctcagg cttcatccac gaggaccacc tgagggagct 421 gctcaccacc atgggcgacc gattcacgga tgaggaggtg gacgagatgt accgcgaggc 481 acccattgat aagaagggca acttcaacta cgtggagttc actcgcatcc tcaaacacgg 541 cgccaaggac aaggacgacc tggatccacc ggtcgccacc atggtgagca agggcgagga 601 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 661 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 721 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta 781 cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc 841 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 901 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 961 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 1021 cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa 1081 gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 1141 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa 1201 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 1261 cgccgggatc actctcggca tggacgagct gtacaagtaa gcggccgcga ctctagatca 1321 taatcagcca taccacatt //