LOCUS Exported 5133 bp ds-DNA linear SYN 04-JUN-2021 DEFINITION Localization: Cilium, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-Patched1-N-18 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5133) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 5133) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 4, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5133 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 4378..4387 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4384..5103 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4429..4450) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4690..4709) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5037..5058 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 tgaacgtcag atccggctag caccgccatg gcctcggctg gtaacgccgc cggggccctg 61 ggcaggcagg ccggcggcgg gaggcgcaga cggaccgggg gaccgcaccg cgccgcgccg 121 gaccgggact atctgcaccg gcccagctac tgcgacgccg ccttcgctct ggagcagatt 181 tccaagggga aggctactgg ccggaaagcg ccgctgtggc tgagagcgaa gtttcagaga 241 ctcttattta aactgggttg ttacattcaa aagaactgcg gcaagttttt ggttgtgggt 301 ctcctcatat ttggggcctt cgctgtggga ttaaaggcag ctaatctcga gaccaacgtg 361 gaggagctgt gggtggaagt tggtggacga gtgagtcgag aattaaatta tacccgtcag 421 aagataggag aagaggctat gtttaatcct caactcatga tacagactcc aaaagaagaa 481 ggcgctaatg ttctgaccac agaggctctc ctgcaacacc tggactcagc actccaggcc 541 agtcgtgtgc acgtctacat gtataacagg caatggaagt tggaacattt gtgctacaaa 601 tcaggggaac ttatcacgga gacaggttac atggatcaga taatagaata cctttaccct 661 tgcttaatca ttacaccttt ggactgcttc tgggaagggg caaagctaca gtccgggaca 721 gcatacctcc taggtaagcc tcctttacgg tggacaaact ttgacccctt ggaattccta 781 gaagagttaa agaaaataaa ctaccaagtg gacagctggg aggaaatgct gaataaagcc 841 gaagttggcc atgggtacat ggaccggcct tgcctcaacc cagccgaccc agattgccct 901 gccacagccc ctaacaaaaa ttcaaccaaa cctcttgatg tggcccttgt tttgaatggt 961 ggatgtcaag gtttatccag gaagtatatg cattggcagg aggagttgat tgtgggtggt 1021 accgtcaaga atgccactgg aaaacttgtc agcgctcacg ccctgcaaac catgttccag 1081 ttaatgactc ccaagcaaat gtatgaacac ttcaggggct acgactatgt ctctcacatc 1141 aactggaatg aagacagggc agccgccatc ctggaggcct ggcagaggac ttacgtggag 1201 gtggttcatc aaagtgtcgc cccaaactcc actcaaaagg tgcttccctt cacaaccacg 1261 accctggacg acatcctaaa atccttctct gatgtcagtg tcatccgagt ggccagcggc 1321 tacctactga tgcttgccta tgcctgttta accatgctgc gctgggactg ctccaagtcc 1381 cagggtgccg tggggctggc tggcgtcctg ttggttgcgc tgtcagtggc tgcaggattg 1441 ggcctctgct ccttgattgg catttctttt aatgctgcga caactcaggt tttgccgttt 1501 cttgctcttg gtgttggtgt ggatgatgtc ttcctcctgg cccatgcatt cagtgaaaca 1561 ggacagaata agaggattcc atttgaggac aggactgggg agtgcctcaa gcgcaccgga 1621 gccagcgtgg ccctcacctc catcagcaat gtcaccgcct tcttcatggc cgcattgatc 1681 cctatccctg ccctgcgagc gttctccctc caggctgctg tggtggtggt attcaatttt 1741 gctatggttc tgctcatttt tcctgcaatt ctcagcatgg atttatacag acgtgaggac 1801 agaagattgg atattttctg ctgtttcaca agcccctgtg tcagcagggt gattcaagtt 1861 gagccacagg cctacacaga gcctcacagt aacacccggt acagcccccc acccccatac 1921 accagccaca gcttcgccca cgaaacccat atcactatgc agtccaccgt tcagctccgc 1981 acagagtatg accctcacac gcacgtgtac tacaccaccg ccgagccacg ctctgagatc 2041 tctgtacagc ctgttaccgt cacccaggac aacctcagct gtcagagtcc cgagagcacc 2101 agctctacca gggacctgct ctcccagttc tcagactcca gcctccactg cctcgagccc 2161 ccctgcacca agtggacact ctcttcgttt gcagagaagc actatgctcc tttcctcctg 2221 aaacccaaag ccaaggttgt ggtaatcctt cttttcctgg gcttgctggg ggtcagcctt 2281 tatgggacca cccgagtgag agacgggctg gacctcacgg acattgttcc ccgggaaacc 2341 agagaatatg acttcatagc tgcccagttc aagtacttct ctttctacaa catgtatata 2401 gtcacccaga aagcagacta cccgaatatc cagcacctac tttacgacct tcataagagt 2461 ttcagcaatg tgaagtatgt catgctggag gagaacaagc aacttcccca aatgtggctg 2521 cactacttta gagactggct tcaaggactt caggatgcat ttgacagtga ctgggaaact 2581 gggaggatca tgccaaacaa ttataaaaat ggatcagatg acggggtcct cgcttacaaa 2641 ctcctggtgc agactggcag ccgagacaag cccatcgaca ttagtcagtt gactaaacag 2701 cgtctggtag acgcagatgg catcattaat ccgagcgctt tctacatcta cctgaccgct 2761 tgggtcagca acgaccctgt agcttacgct gcctcccagg ccaacatccg gcctcaccgg 2821 ccggagtggg tccatgacaa agccgactac atgccagaga ccaggctgag aatcccagca 2881 gcagagccca tcgagtacgc tcagttccct ttctacctca acggcctacg agacacctca 2941 gactttgtgg aagccataga aaaagtgaga gtcatctgta acaactatac gagcctggga 3001 ctgtccagct accccaatgg ctaccccttc ctgttctggg agcaatacat cagcctgcgc 3061 cactggctgc tgctatccat cagcgtggtg ctggcctgca cgtttctagt gtgcgcagtc 3121 ttcctcctga acccctggac ggccgggatc attgtcatgg tcctggctct gatgaccgtt 3181 gagctctttg gcatgatggg cctcattggg atcaagctga gtgctgtgcc tgtggtcatc 3241 ctgattgcat ctgttggcat cggagtggag ttcaccgtcc acgtggcttt ggcctttctg 3301 acagccattg gggacaagaa ccacagggct atgctcgctc tggagcacat gtttgctccc 3361 gttctggacg gtgctgtgtc cactctgctg ggtgtactga tgcttgcagg gtccgaattt 3421 gatttcattg tcagatactt ctttgccgtc ctggccattc tcaccgtctt gggggttctc 3481 aatggactgg ttctgctgcc tgtcctctta tccttctttg gaccgtgtcc tgaggtgtct 3541 ccagccaatg gcctaaaccg actgcccact ccttcgcctg agccgcctcc aagtgtcgtc 3601 cggtttgccg tgcctcctgg tcacacgaac aatgggtctg attcctccga ctcggagtac 3661 agctctcaga ccacggtgtc tggcatcagt gaggagctca ggcaatacga agcacagcag 3721 ggtgccggag gccctgccca ccaagtgatt gtggaagcca cagaaaaccc tgtctttgcc 3781 cggtccactg tggtccatcc ggactccaga catcagcctc ccttgacccc tcggcaacag 3841 ccccacctgg actctggctc cttgtcccct ggacggcaag gccagcagcc tcgaagggat 3901 ccccctagag aaggcttgcg gccacccccc tacagaccgc gcagagacgc ttttgaaatt 3961 tctactgaag ggcattctgg ccctagcaat agggaccgct cagggccccg tggggcccgt 4021 tctcacaacc ctcggaaccc aacgtccacc gccatgggca gctctgtgcc cagctactgc 4081 cagcccatca ccactgtgac ggcttctgct tcggtgactg ttgctgtgca tcccccgcct 4141 ggacctgggc gcaacccccg aggggggccc tgtccaggct atgagagcta ccctgagact 4201 gatcacgggg tatttgagga tcctcatgtg ccttttcatg tcaggtgtga gaggagggac 4261 tcaaaggtgg aggtcataga gctacaggac gtggaatgtg aggagaggcc gtgggggagc 4321 agctccaacg ggagaggcag cgggagtggc agtcgcggag gcagtggtgg accggtcgcc 4381 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 4441 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 4501 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 4561 accctcgtga ccaccttgac ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg 4621 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 4681 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 4741 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 4801 cacaagctgg agtacaacta caacagccac aaggtctata tcaccgccga caagcagaag 4861 aacggcatca aggtgaactt caagacccgc cacaacatcg aggacggcag cgtgcagctc 4921 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 4981 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg 5041 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 5101 taagcggccg cgactctaga tcataatcag cca //