LOCUS       Exported                1467 bp ds-DNA     linear   SYN 01-JUN-2021
DEFINITION  Localization: Ras GTPase, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    mEmerald-Rab11a-7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1467)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 1, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      29..38
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             35..751
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ccatgggcac
      781 ccgcgacgac gagtacgact acctctttaa agttgtcctt attggagatt ctggtgttgg
      841 aaagagtaat ctcctgtctc gatttactcg aaatgagttt aatctggaaa gcaagagcac
      901 cattggagta gagtttgcaa caagaagcat ccaggttgat ggaaaaacaa taaaggcaca
      961 gatatgggac acagcagggc aagagcgata tcgagctata acatcagcat attatcgtgg
     1021 agctgtaggt gccttattgg tttatgacat tgctaaacat ctcacatatg aaaatgtaga
     1081 gcgatggctg aaagaactga gagatcatgc tgatagtaac attgttatca tgcttgtggg
     1141 caataagagt gatctacgtc atctcagggc agttcctaca gatgaagcaa gagcttttgc
     1201 agaaaagaat ggtttgtcat tcattgaaac ttcggcccta gactctacaa atgtagaagc
     1261 tgcttttcag acaattttaa cagagattta ccgcattgtt tctcagaagc aaatgtcaga
     1321 cagacgcgaa aatgacatgt ctccaagcaa caatgtggtt cctattcatg ttccaccaac
     1381 cactgaaaac aagccaaagg tgcagtgctg tcagaacatc taaggatcca ccggatctag
     1441 ataactgatc ataatcagcc ataccac
//
