LOCUS Exported 1934 bp ds-DNA linear SYN 31-MAY-2021 DEFINITION Localization: Membrane Scaffold, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-Sequestosome1-C-18 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1934) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 1934) AUTHORS . TITLE Direct Submission JOURNAL Exported May 31, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1934 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 31..40 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 37..753 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(82..103) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(343..362) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 690..711 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg 61 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 121 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc 241 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 301 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 481 cacaaggtct atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc 541 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 601 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg 661 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 721 gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg agagagcgga 781 ggtagcgcat ccggcggaag cggaggagcc atgtcctacg tgaaggatga catcttccga 841 atctacatta aagagaaaaa agagtgccgg cgggaccacc gcccaccgtg tgctcaggag 901 gcgccccgca acatggtgca ccccaatgtg atctgcgatg gctgcaatgg gcctgtggta 961 ggaacccgct acaagtgcag cgtctgccca gactacgact tgtgtagcgt ctgcgaggga 1021 aagggcttgc accgggggca caccaagctc gcattcccca gccccttcgg gcacctgtct 1081 gagggcttct cgcacagccg ctggctccgg aaggtgaaac acggacactt cgggtggcca 1141 ggatgggaaa tgggtccacc aggaaactgg agcccacgtc ctcctcgtgc aggggaggcc 1201 cgccctggcc ccacggcaga atcagcttct ggtccatcgg aggatccgag tgtgaatttc 1261 ctgaagaacg ttggggagag tgtggcagct gcccttagcc ctctgggcat tgaagttgat 1321 atcgatgtgg agcacggagg gaaaagaagc cgcctgaccc ccgtctctcc agagagttcc 1381 agcacagagg agaagagcag ctcacagcca agcagctgct gctctgatcc cagcaagccg 1441 ggtgggaatg ttgagggcgc cacgcagtct ctggcggagc agatgagaaa gatcgccttg 1501 gagtccgagg ggcgccctga ggaacagatg gagtcggata actgttcagg aggagatgat 1561 gactggaccc atctgtcttc aaaagaagtg gacccgtcta caggtgaact ccagtcccta 1621 cagatgccag aatccgaagg gccaagctct ctggacccct cccaggaggg acccacaggg 1681 ctgaaggaag ctgccttgta cccacatctc ccgccagagg ctgacccgcg gctgattgag 1741 tccctctccc agatgctgtc catgggcttc tctgatgaag gcggctggct caccaggctc 1801 ctgcagacca agaactatga catcggagcg gctctggaca ccatccagta ttcaaagcat 1861 cccccgccgt tgtgagaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc 1921 tagataactg atca //