LOCUS Exported 1587 bp ds-DNA linear SYN 05-JUN-2021 DEFINITION Localization: Kinetochore, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-Ska1-C-18 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1587) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 1587) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 5, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1587 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 20..29 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 26..742 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(71..92) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(332..351) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 679..700 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt 61 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 121 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 181 caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg tgcagtgctt 241 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 301 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 361 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 421 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta 481 tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat 541 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 601 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc 661 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 721 cggcatggac gagctgtaca agtccggaag tgctggcagc ggtggaggca gcgcatccgg 781 cggaggcagc ggaagcgcct cgtcagatct ggaacaatta tgctctcatg ttaatgaaaa 841 gattggcaat attaagaaaa ccttatcatt aagaaactgt ggccaggaac ctaccttgaa 901 aactgtatta aataaaatag gagatgagat cattgtaata aatgaacttc taaataaatt 961 ggaattggaa attcagtatc aagaacaaac caacaattca ctcaaggaac tctgtgaatc 1021 tcttgaagaa gattacaaag acatagaaca tcttaaagaa aacggtcctt cccatttgcc 1081 tcaagtaaca gtaacccaga gctgtgttaa gggatcagat cttgatcctg aagaaccaat 1141 caaagttgaa gaacctgaac ccgtaaagaa gcctcccaaa gagcaaagaa gtattaagga 1201 aatgccattt ataacttgtg atgagttcaa tggtgttcct tcgtacatga aatcccgctt 1261 aacctataat caaattaatg atgttattaa agaaatcaac aaggcagtaa ttagtaaata 1321 taaaatccta catcagccaa aaaagtctat gaattctgtg accagaaatc tctatcacag 1381 atttattgat gaagaaacga aggataccaa aggtcgttat tttatagtgg aagctgacat 1441 aaaggagttc acaactttga aagctgacaa gaagtttcgc gtgttactga atattttacg 1501 acactgccgg aggctatcag aggtccgagg gggaggactt actcgttatg ttataacctg 1561 aggatccacc ggatctagat aactgat //