LOCUS Exported 1721 bp ds-DNA linear SYN 08-JUN-2021 DEFINITION Localization: Membrane, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-Stomatin-N-16 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1721) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 1721) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 8, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1721 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 41..50 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 953..962 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 959..1678 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1004..1025) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1265..1284) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1612..1633 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 gacgtcagat ccgctagcgc taccggactc agatctcgag gccaccatgg ccgagaagcg 61 gcacacacgg gactccgaag cccagcggct ccccgactcc ttcaaggaca gccccagtaa 121 gggccttgga ccttgcggat ggattttggt ggcgttctca ttcttattca ccgttataac 181 tttcccaatc tcaatatgga tgtgcataaa gattataaaa gagtatgaaa gagccatcat 241 ctttagattg ggtcgcattt tacaaggagg agccaaagga cctggtttgt tttttattct 301 gccatgcact gacagcttca tcaaagtgga catgagaact atttcatttg atattcctcc 361 tcaggagatc ctcacaaagg attcagtgac aattagcgtg gatggtgtgg tctattaccg 421 cgttcagaat gcaaccctgg ctgtggcaaa tatcaccaac gctgactcag caacccgtct 481 tttggcacaa actactctga ggaatgttct gggcaccaag aatctttctc agatcctctc 541 tgacagagaa gaaattgcac acaacatgca gtctactctg gatgatgcca ctgatgcctg 601 gggaataaag gtggagcgtg tggaaattaa ggatgtgaaa ctacctgtgc agctccagag 661 agctatggct gcagaagcag aagcgtcccg cgaggcccgc gccaaggtta ttgcagccga 721 aggagaaatg aatgcatcca gggctctgaa agaagcctcc atggtcatca ctgaatctcc 781 tgcagccctt cagctccgat acctgcagac actgaccacc attgctgctg agaaaaactc 841 aacaattgtc ttccctctgc ccatagatat gctgcaagga atcatagggg caaaacacag 901 ccatctaggc gcatccgggg gtgcgtcagg cggcagcgga tctggaccgg tcgccaccat 961 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 1021 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 1081 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 1141 cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca 1201 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 1261 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 1321 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 1381 gctggagtac aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg 1441 catcaaggtg aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga 1501 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 1561 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 1621 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc 1681 ggccgcgact ctagatcata atcagccata ccacattgta g //