LOCUS Exported 1260 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Localization: Nucleus, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-TOMM20-C-10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1260) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 1260) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1260 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 26..35 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 32..748 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(77..98) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(338..357) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 685..706 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 cgtcagatcg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac 61 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca 241 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 481 ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca 541 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa 661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 721 cactctcggc atggacgagc tgtacaagtc cggactcaga tctagcgcaa gtggcggagt 781 gggtcggaac agcgccatcg ccgccggtgt atgcggggcc cttttcattg ggtactgcat 841 ctacttcgac cgcaaaagac gaagtgaccc caacttcaag aacaggcttc gagaacgaag 901 aaagaaacag aagcttgcca aggagagagc tgggctttcc aagttacctg accttaaaga 961 tgctgaagct gttcagaagt tcttccttga agaaatacag cttggtgaag agttactagc 1021 tcaaggtgaa tatgagaagg gcgtagacca tctgacaaat gcaattgctg tgtgtggaca 1081 gccacagcag ttactgcagg tcttacagca aactcttcca ccaccagtgt tccagatgct 1141 tctgactaag ctcccaacaa ttagtcagag aattgtaagt gctcagagct tggctgaaga 1201 tgatgtggaa tgaggatcca ccggatctag ataactgatc ataatcagcc ataccacatt //