LOCUS Exported 2840 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION Localization: Focal Adhesions, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-Vinexin-N-12 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2840) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 2840) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2840 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 2075..2084 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2081..2800 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2126..2147) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2387..2406) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2734..2755 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 gctagtgacg tcagatccgc tagcagccac catgcagggc ccaccccgca gcctccgcgc 61 tgggctcagc ctggacgact tcatccctgg ccacctccag tcccacatag ggtcttcctc 121 ccgggggaca cgggtgcccg tgatccggaa tggtggctcc aacaccctta atttccagtt 181 ccacgacccc gcgcccagga ctgtgtgcaa tgggggctac acaccaagac gagatgcttc 241 ccagcacccg gaccctgcgt ggtatcagac ctggccaggc cctgggagca agccctctgc 301 aagcacaaag atccctgcct cccagcacac ccagaactgg tcagccacgt ggaccaagga 361 cagcaagcgt cgggacaagc gctgggtcaa gtacgaggga atcgggcccg tggacgagag 421 cggcatgccc attgcccccc gatccagcgt tgacagaccc agagactggt accggagaat 481 gttccagcag attcaccgga aaatgccaga cttgcagctg gactggacct tcgaggagcc 541 acccagagac cccaggcatc taggagccca gcaaagacct gcccacaggc ccggcccggc 601 aacatcttcc agtggaagaa gctgggacca ctctgaagag ttacctagaa gcaccttcaa 661 ctacagacct ggagcattct ccactgtgct gcagccctca aatcaggtgc tcagacgccg 721 ggaaaaagta gacaatgtct ggacggaaga gtcctggaac cagtttctgc aggaactaga 781 gactgggcag aggcccaaga aaccgctggt ggacgaccct ggtgagaagc cctcccagcc 841 cattgaggtg ctgctggaga gagagctggc cgagctgagc gccgagctgg acaaggacct 901 gcgggcaatt gagacccgac tgccgtcccc caagagctcg ccggcgcccc gacgggcccc 961 ggagcagcgg cccccggccg gcccggcctc agcctggagc tccagctacc cacatgcacc 1021 ttacctgggt tccgcccggt ccctgagtcc ccacaaaatg gctgatggag gaagcccctt 1081 cctaggtcgg agggactttg tctacccttc ctcaacccga gaccctagtg cctctaacgg 1141 agggggcagc ccagccagga gggaagagaa gaagagaaag gccgccaggc tcaagtttga 1201 cttccaggcg cagtccccca aggagctgac tctgcagaag ggtgacattg tctacatcca 1261 caaggaggtg gacaagaact ggctggaggg agagcaccac ggccgcctgg gcatcttccc 1321 tgctaattat gtggaggtgc tgcccgcaga tgagatccct aagcccatca agcccccgac 1381 ctaccaggtg ctggagtatg gagaggctgt ggcccagtac accttcaagg gggacctgga 1441 ggtggagctg tccttccgca agggagagca catctgcctg atccgcaagg tgaacgagaa 1501 ctggtacgag ggacgcatca cgggcacggg gcgccaaggc atattccctg ccagctacgt 1561 gcaggtgtct cgtgaacccc ggctccggct ctgtgacgac ggcccccagc tccccacgtc 1621 tccccgcctg accgctgccg cccgctcagc ccgtcacccc agctccccct cagccctgcg 1681 cagcccagct gaccccaccg acttgggggg acagacctcc ccccgtcgca ctggcttctc 1741 cttccccacc caggagccta gaccccagac ccagaatctt ggcacccctg gtccagctct 1801 gtcccactct cgaggtccca gccatcccct ggacctgggg acctcctctc ctaacacctc 1861 tcagatacac tggaccccgt accgggcgat gtaccagtac aggccccaga acgaagacga 1921 gctggagctg cgcgaggggg acagggtgga tgtcatgcag cagtgtgacg atggctggtt 1981 tgtgggtgtc tcccggagga cccagaaatt cggaacgttc cctggaaatt acgttgcccc 2041 ggtggcatcc ggcggtagcg gggatccacc ggtcgccacc atggtgagca agggcgagga 2101 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 2161 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 2221 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta 2281 cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc 2341 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 2401 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 2461 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 2521 cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa 2581 gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 2641 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa 2701 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 2761 cgccgggatc actctcggca tggacgagct gtacaagtaa gcggccgcga ctctagatca 2821 taatcagcca taccacattg //