LOCUS Exported 994 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Localization: N1 Cloning Vector, Excitation: 558, Emission: 583. ACCESSION . VERSION . KEYWORDS DsRed2-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 994) TITLE Michael Davidson Empty Backbones REFERENCE 2 (bases 1 to 994) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..994 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 28..79 /label=CMVd2 promoter /note="truncated human cytomegalovirus (CMV) immediate early promoter that yields moderately reduced expression relative to the full-length promoter" primer_bind 30..50 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" misc_feature 102..182 /label=MCS /note="multiple cloning site" regulatory 184..193 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 190..867 /codon_start=1 /product="improved tetrameric variant of DsRed fluorescent protein" /label=DsRed2 /note="mammalian codon-optimized" /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH LFL" primer_bind complement(370..390) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 782..805 /label=DsRed1-C /note="DsRed1, forward primer" ORIGIN 1 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 61 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca 121 gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg 181 gtcgccacca tggcctcctc cgagaacgtc atcaccgagt tcatgcgctt caaggtgcgc 241 atggagggca ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc 301 tacgagggcc acaacaccgt gaagctgaag gtgaccaagg gcggccccct gcccttcgcc 361 tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc 421 gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg 481 aacttcgagg acggcggcgt ggcgaccgtg acccaggact cctccctgca ggacggctgc 541 ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc cgtgatgcag 601 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg 661 aagggcgaga cccacaaggc cctgaagctg aaggacggcg gccactacct ggtggagttc 721 aagtccatct acatggccaa gaagcccgtg cagctgcccg gctactacta cgtggacgcc 781 aagctggaca tcacctccca caacgaggac tacaccatcg tggagcagta cgagcgcacc 841 gagggccgcc accacctgtt cctgtaagcg gccgcgactc tagatcataa tcagccatac 901 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa 961 acataaaatg aatgcaattg ttgttgttaa cttg //