LOCUS       Exported                1147 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Localization: N1 Cloning Vector, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    mTFP1-N1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        12..111
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     62..82
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    134..214
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      216..225
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             222..932
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     polyA_signal    1054..1134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1091..1110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tttgtttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
       61 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
      121 aaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc
      181 agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc aagggcgagg
      241 agaccacaat gggcgtaatc aagcccgaca tgaagatcaa gctgaagatg gagggcaacg
      301 tgaatggcca cgccttcgtg atcgagggcg agggcgaggg caagccctac gacggcacca
      361 acaccatcaa cctggaggtg aaggagggag cccccctgcc cttctcctac gacattctga
      421 ccaccgcgtt cgcctacggc aacagggcct tcaccaagta ccccgacgac atccccaact
      481 acttcaagca gtccttcccc gagggctact cttgggagcg caccatgacc ttcgaggaca
      541 agggcatcgt gaaggtgaag tccgacatct ccatggagga ggactccttc atctacgaga
      601 tacacctcaa gggcgagaac ttccccccca acggccccgt gatgcagaag aagaccaccg
      661 gctgggacgc ctccaccgag aggatgtacg tgcgcgacgg cgtgctgaag ggcgacgtca
      721 agcacaagct gctgctggag ggcggcggcc accaccgcgt tgacttcaag accatctaca
      781 gggccaagaa ggcggtgaag ctgcccgact atcactttgt ggaccaccgc atcgagatcc
      841 tgaaccacga caaggactac aacaaggtga ccgtttacga gagcgccgtg gcccgcaact
      901 ccaccgacgg catggacgag ctgtacaagt aagcggccgc gactctagat cataatcagc
      961 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     1021 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     1081 tacaaataaa gcaatagcat cacaaatttc acaataaagc atttttttca ctgcattcta
     1141 gttgtgg
//
