LOCUS Exported 3662 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pGEM-LIN28 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3662) AUTHORS Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA TITLE Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. JOURNAL Science. 2007 Nov 20. ():. PUBMED 18029452 REFERENCE 2 (bases 1 to 3662) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3662 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 33..42 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 37..46 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter complement(787..805) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(788..805) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(823..839) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(823..839) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(836..858) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 847..863 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(871..901) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 916..937 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1054..1071) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1225..1813) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1305..1324) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1984..2844) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2607..2626 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2845..2949) /gene="bla" /label=AmpR promoter rep_origin complement(3027..3482) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3164..3185) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3376..3395 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3608..3630 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3622..3639 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3623..3639 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3646..3 /label=T7 /note="T7 promoter, forward primer" promoter 3646..2 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat 61 tatcgccatg ggctccgtgt ccaaccagca gtttgcaggt ggctgcgcca aggcggcaga 121 agaggcgccc gaggaggcgc cggaggacgc ggcccgggcg gcggacgagc ctcagctgct 181 gcacggtgcg ggcatctgta agtggttcaa cgtgcgcatg gggttcggct tcctgtccat 241 gaccgcccgc gccggggtcg cgctcgaccc cccagtggat gtctttgtgc accagagtaa 301 gctgcacatg gaagggttcc ggagcttgaa ggagggtgag gcagtggagt tcacctttaa 361 gaagtcagcc aagggtctgg aatccatccg tgtcaccgga cctggtggag tattctgtat 421 tgggagtgag aggcggccaa aaggaaagag catgcagaag cgcagatcaa aaggagacag 481 gtgctacaac tgtggaggtc tagatcatca tgccaaggaa tgcaagctgc caccccagcc 541 caagaagtgc cacttctgcc agagcatcag ccatatggta gcctcatgtc cgctgaaggc 601 ccagcagggc cctagtgcac agggaaagcc aacctacttt cgagaggaag aagaagaaat 661 ccacagccct accctgctcc cggaggcaca gaattgagtt taaacnaatc actagtgaat 721 tcgcggccgc ctgcaggtcg accatatggg agagctccca acgcgttgga tgcatagctt 781 gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt 841 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 901 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 961 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 1021 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 1081 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 1141 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 1201 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 1261 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 1321 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 1381 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 1441 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 1501 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 1561 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 1621 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 1681 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 1741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 1801 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 1861 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 1921 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 1981 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 2041 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 2101 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 2161 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 2221 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 2281 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 2341 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 2401 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 2461 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 2521 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 2581 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 2641 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 2701 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 2761 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 2821 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 2881 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 2941 ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat 3001 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc 3061 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 3121 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 3181 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 3241 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 3301 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 3361 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 3421 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 3481 gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 3541 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca 3601 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta 3661 ta //