LOCUS       Exported                3983 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGEM-Sox2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, 
            Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, 
            Thomson JA
  TITLE     Induced Pluripotent Stem Cell Lines Derived from Human Somatic 
            Cells.
  JOURNAL   Science. 2007 Nov 20. ():.
  PUBMED    18029452
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      33..42
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      37..46
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(1108..1126)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1109..1126)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1144..1160)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1144..1160)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1157..1179)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1168..1184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1192..1222)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1237..1258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1375..1392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1546..2134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1626..1645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2305..3165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2928..2947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3166..3270)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3348..3803)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3485..3506)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3697..3716
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3929..3951
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3943..3960
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3944..3960
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3967..3
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3967..2
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tccgcatgta caacatgatg gagacggagc tgaagccgcc gggcccgcag caaacttcgg
      121 ggggcggcgg cggcaactcc accgcggcgg cggccggcgg caaccagaaa aacagcccgg
      181 accgcgtcaa gcggcccatg aatgccttca tggtgtggtc ccgcgggcag cggcgcaaga
      241 tggcccagga gaaccccaag atgcacaact cggagatcag caagcgcctg ggcgccgagt
      301 ggaaactttt gtcggagacg gagaagcggc cgttcatcga cgaggctaag cggctgcgag
      361 cgctgcacat gaaggagcac ccggattata aataccggcc ccggcggaaa accaagacgc
      421 tcatgaagaa ggataagtac acgctgcccg gcgggctgct ggcccccggc ggcaatagca
      481 tggcgagcgg ggtcggggtg ggcgccggcc tgggcgcggg cgtgaaccag cgcatggaca
      541 gttacgcgca catgaacggc tggagcaacg gcagctacag catgatgcag gaccagctgg
      601 gctacccgca gcacccgggc ctcaatgcgc acggcgcagc gcagatgcag cccatgcacc
      661 gctacgacgt gagcgccctg cagtacaact ccatgaccag ctcgcagacc tacatgaacg
      721 gctcgcccac ctacagcatg tcctactcgc agcagggcac ccctggcatg gctcttggct
      781 ccatgggttc ggtggtcaag tccgaggcca gctccagccc ccctgtggtt acctcttcct
      841 cccactccag ggcgccctgc caggccgggg acctccggga catgatcagc atgtatctcc
      901 ccggcgccga ggtgccggaa cccgccgccc ccagcagact tcacatgtcc cagcactacc
      961 agagcggccc ggtgcccggc acggccatta acggcacact gcccctctca cacatgtgag
     1021 ggccggaaat cactagtgaa ttcgcggccg cctgcaggtc gaccatatgg gagagctccc
     1081 aacgcgttgg atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc
     1141 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     1201 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1261 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     1321 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1381 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1441 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1501 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1561 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1621 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1681 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1741 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1801 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1861 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1921 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1981 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2041 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2101 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2161 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2221 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2281 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2341 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2401 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2461 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2521 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2581 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2641 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2701 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2761 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2821 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     2881 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     2941 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3001 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3061 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3121 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3181 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3241 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgatgc
     3301 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt
     3361 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3421 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     3481 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     3541 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3601 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     3661 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     3721 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3781 taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg
     3841 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     3901 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     3961 gaattgtaat acgactcact ata
//
