LOCUS Exported 1009 bp ds-DNA linear SYN 31-MAY-2021 DEFINITION Localization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593. ACCESSION . VERSION . KEYWORDS mKikGR-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1009) TITLE Michael Davidson Empty Backbones REFERENCE 2 (bases 1 to 1009) AUTHORS . TITLE Direct Submission JOURNAL Exported May 31, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1009 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 14..94 /label=MCS /note="multiple cloning site" regulatory 96..105 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 102..803 /codon_start=1 /product="monomeric photoconvertible Kikume green-to-red fluorescent protein" /label=mKikGR1 /translation="MVSVITSEMKIELRMEGSVNGHKFVITGKGSGRPYEGTQTVDLTV IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQLFPEGYSWERSMSYEDGGICLA TNNITMKKDGSNTFVNEIRFDGTNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVEM ALLLEGGGHYRCDFRTTYKAKKVVQLPDYHYVDHQMEITSHDKDYNKVKAYEHAKAYSG TYRGAKYEFEA" polyA_signal 925..1003 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(961..980) /label=SV40pA-R /note="SV40 polyA, reverse primer" ORIGIN 1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc 61 agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagt gtgattacat 121 cagaaatgaa gatcgagctg cgtatggaag gctctgtaaa cgggcacaag ttcgtgatta 181 cagggaaagg aagtggcaga ccttacgagg gaacacagac tgtggacctg acagtcatag 241 agggcggacc tcttcctttt gctttcgata tcctgacaac agcattccat tacggcaacc 301 gggtatttgt cgaataccca gaagaaatag tagactactt caagcagttg tttcctgagg 361 gttattcttg ggaacgaagc atgagttacg aagacggggg aatttgcctc gccacaaaca 421 atataacgat gaagaaagac ggcagcaaca cttttgtcaa tgaaattcga tttgatggta 481 caaactttcc tgccaatggt ccagttatgc agaggaagac cgtcaaatgg gagccatcca 541 ctgagaaaat gtatgtgcgt gatggagtgc tgaagggtga tgttgaaatg gctctgttgc 601 ttgaaggagg tggccattac cgatgtgact tcagaactac ttacaaagca aagaaggttg 661 tccagttgcc agactatcac tacgtggatc atcaaatgga gataacaagc catgacaagg 721 attacaacaa ggttaaggcg tatgaacatg ctaaggctta ttccgggacc tacaggggcg 781 ccaagtatga atttgaagcc taagcggccg cgactctaga tcataatcag ccataccaca 841 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 901 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaataaa 961 gcaatagcat cacaaatttc acaaataagc attttttcac tgcattcta //