LOCUS Exported 994 bp ds-DNA linear SYN 21-MAY-2021 DEFINITION Localization: C1 Cloning Vector, Excitation: 559, Emission: 600. ACCESSION . VERSION . KEYWORDS mRuby2-C1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 994) AUTHORS Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ TITLE Improving FRET dynamic range with bright green and red fluorescent proteins. JOURNAL Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. PUBMED 22961245 REFERENCE 2 (bases 1 to 994) AUTHORS . TITLE Direct Submission JOURNAL Exported May 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..994 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 3..54 /label=CMVd2 promoter /note="truncated human cytomegalovirus (CMV) immediate early promoter that yields moderately reduced expression relative to the full-length promoter" primer_bind 5..25 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 93..102 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 99..809 /codon_start=1 /product="monomeric red fluorescent protein derived from mRuby, with improved photophysical properties (Lam et al., 2012)" /label=mRuby2 /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV AKFAGLGGGMDELYK" misc_feature 810..875 /label=MCS /note="multiple cloning site" ORIGIN 1 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta 61 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgtctaag ggcgaagagc 121 tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat 181 tcaaatgcac aggtgaagga gaaggcaatc cgtacatggg aactcaaacc atgaggatca 241 aagtcatcga gggaggaccc ctgccatttg cctttgacat tcttgccacg tcgttcatgt 301 atggcagccg tacttttatc aagtacccga aaggcattcc tgatttcttt aaacagtcct 361 ttcctgaggg ttttacttgg gaaagagtta cgagatacga agatggtgga gtcgtcaccg 421 tcatgcagga caccagcctt gaggatggct gtctcgttta ccacgtccaa gtcagagggg 481 taaactttcc ctccaatggt cccgtgatgc agaagaagac caagggttgg gagcctaata 541 cagagatgat gtatccagca gatggtggtc tgaggggata cactcatatg gcactgaaag 601 ttgatggtgg tggccatctg tcttgctctt tcgtaacaac ttacaggtca aaaaagaccg 661 tcgggaacat caagatgccc ggtatccatg ccgttgatca ccgcctggaa aggttagagg 721 aaagtgacaa tgaaatgttc gtagtacaac gcgaacacgc agttgccaag ttcgccgggc 781 ttggtggtgg gatggacgag ctgtacaagt ccggactcag atctcgagct caagcttcga 841 attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa 901 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc 961 tgaacctgaa acataaaatg aatgcaattg ttgt //