LOCUS Exported 994 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581. ACCESSION . VERSION . KEYWORDS mEos4b-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 994) AUTHORS Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, Wang Y, Looger LL TITLE Fixation-resistant photoactivatable fluorescent proteins for CLEM. JOURNAL Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225. PUBMED 25581799 REFERENCE 2 (bases 1 to 994) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..994 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 28..79 /label=CMVd2 promoter /note="truncated human cytomegalovirus (CMV) immediate early promoter that yields moderately reduced expression relative to the full-length promoter" primer_bind 30..50 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" misc_feature 102..182 /label=MCS /note="multiple cloning site" regulatory 184..193 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 190..873 /codon_start=1 /product="fixation-resistant truly monomeric green-to-red photoswitchable fluorescent protein (Paez-Segala et al., 2015)" /label=mEos4b /note="derived from mEos2" /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP DNARR" ORIGIN 1 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 61 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca 121 gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg 181 gtcgccacca tggtgagtgc gattaagcca gacatgagga tcaaactccg tatggaaggc 241 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttatgaggga 301 aaacagacca tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc 361 ctgaccactg cattccatta cggcaacagg gtattcgtga aatatccaga caacatacaa 421 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa 481 gacgggggca tttgcaatgc cagaaacgac ataacaatgg aaggggacac tttctataat 541 aaagttcgat tttatggtac caactttccc gccaatggtc cagttatgca gaagaagacg 601 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gacgggtgat 661 attgagatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact 721 tacaaagcta aggagaaggg tgtcaagtta ccaggcgccc actttgtgga ccacgccatt 781 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct 841 cattctggat tgcctgacaa tgccagacga taagcggccg cgactctaga tcataatcag 901 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 961 cctgaaacat aaaatgaatg caattgttgt tgtt //