LOCUS Exported 1136 bp ds-DNA linear SYN 03-JUN-2021 DEFINITION Localization: Endoplasmic Reticulum, Excitation: 568, Emission: 592. ACCESSION . VERSION . KEYWORDS mApple-Sec61-N-18 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1136) TITLE Davidson lab - mApple constructs REFERENCE 2 (bases 1 to 1136) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 3, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1136 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 36..323 /codon_start=1 /gene="SEC61B" /product="human Sec61-beta subunit of the translocon in the endoplasmic reticulum" /label=Sec61-beta /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS" regulatory 372..381 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 378..1088 /codon_start=1 /product="photostable monomeric derivative of DsRed (Shaner et al., 2008)" /label=mApple /note="mammalian codon-optimized" /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind 791..810 /label=mCherry-F /note="mCherry, forward primer" ORIGIN 1 gtcgtgctag tgacgtcaga tccgctagcg ccaccatgcc tggtccgacc cccagtggca 61 ctaacgtggg atcctcaggg cgctctccca gcaaagcagt ggccgcccgg gcggcgggat 121 ccactgtccg gcagaggaaa aatgccagct gtgggacaag gagtgcaggc cgcacaacct 181 cggcaggcac cggggggatg tggcgattct acacagaaga ttcacctggg ctcaaagttg 241 gccctgttcc agtattggtt atgagtcttc tgttcatcgc ttctgtattt atgttgcaca 301 tttggggcaa gtacactcgt tcgggacgaa ttctgcagtc gacggtaccg cgggcccggg 361 atccaccggt cgccaccatg gtgagcaagg gcgaggagaa taacatggcc atcatcaagg 421 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg 481 agggcgaggg cgagggccgc ccctacgagg cctttcagac cgctaagctg aaggtgacca 541 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca 601 aggtctacat taagcaccca gccgacatcc ccgactactt caagctgtcc ttccccgagg 661 gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg cattattcac gttaaccagg 721 actcctccct gcaggacggc gtgttcatct acaaggtgaa gctgcgcggc accaacttcc 781 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc gaggagcgga 841 tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa gaggctgaag ctgaaggacg 901 gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc 961 ccggcgccta catcgtcgac atcaagttgg acatcgtgtc ccacaacgag gactacacca 1021 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt 1081 acaagtaagc ggccgcgact ctagatcata atcagccata ccacattgta gagtcc //