LOCUS       Exported                2603 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    mCherry-Calnexin-N-14
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      1850..1859
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1856..2566
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2001..2019)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2269..2288
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tccgctagcg ctaccggact cagatctcga gaccgccatg gaagggaagt ggttgctgtg
       61 tatgttactg gtgcttggaa ctgctattgt tgaggctcat gatggacatg atgatgatgt
      121 gattgatatt gaggatgacc ttgacgatgt cattgaagag gtagaagact caaaaccaga
      181 taccactgct cctccttcat ctcccaaggt tacttacaaa gctccagttc caacagggga
      241 agtatatttt gctgattctt ttgacagagg aactctgtca gggtggattt tatccaaagc
      301 caagaaagac gataccgatg atgaaattgc caaatatgat ggaaagtggg aggtagagga
      361 aatgaaggag tcaaagcttc caggtgataa aggacttgtg ttgatgtctc gggccaagca
      421 tcatgccatc tctgctaaac tgaacaagcc cttcctgttt gacaccaagc ctctcattgt
      481 tcagtatgag gttaatttcc aaaatggaat agaatgtggt ggtgcctatg tgaaactgct
      541 ttctaaaaca ccagaactca acctggatca gttccatgac aagacccctt atacgattat
      601 gtttggtcca gataaatgtg gagaggacta taaactgcac ttcatcttcc gacacaaaaa
      661 ccccaaaacg ggtatctatg aagaaaaaca tgctaagagg ccagatgcag atctgaagac
      721 ctattttact gataagaaaa cacatcttta cacactaatc ttgaatccag ataatagttt
      781 tgaaatactg gttgaccaat ctgtggtgaa tagtggaaat ctgctcaatg acatgactcc
      841 tcctgtaaat ccttcacgtg aaattgagga cccagaagac cggaagcccg aggattggga
      901 tgaaagacca aaaatcccag atccagaagc tgtcaagcca gatgactggg atgaagatgc
      961 ccctgctaag attccagatg aagaggccac aaaacccgaa ggctggttag atgatgagcc
     1021 tgagtacgta cctgatccag acgcagagaa acctgaggat tgggatgaag acatggatgg
     1081 agaatgggag gctcctcaga ttgccaaccc tagatgtgag tcagctcctg gatgtggtgt
     1141 ctggcagcga cctgtgattg acaaccccaa ttataaaggc aaatggaagc ctcctatgat
     1201 tgacaatccc agttaccagg gaatctggaa acccaggaaa ataccaaatc cagatttctt
     1261 tgaagatctg gaacctttca gaatgactcc ttttagtgct attggtttgg agctgtggtc
     1321 catgacctct gacatttttt ttgacaactt tatcatttgt gctgatcgaa gaatagttga
     1381 tgattgggcc aatgatggat ggggcctgaa gaaagctgct gatggggctg ctgagccagg
     1441 cgttgtgggg cagatgaacg aggcagctga agagcgcccg tggctgtggg tagtctatat
     1501 tctaactgta gcccttcctg tgttcctggt tatcctcttc tgctgttctg gaaagaaaca
     1561 gaccagtggt atggagtata agaaaactga tgcacctcaa ccggatgtga aggaagagga
     1621 agaagagaag gaagaggaaa aggacaaggg agatgaggag gaggaaggag aagagaaact
     1681 tgaagagaaa cagaaaagtg atgctgaaga agatggtggc actgtcagtc aagaggagga
     1741 agacagaaaa cctaaagcag aggaggatga aattttgaac agatcaccaa gaaacagaaa
     1801 gccacgaaga gaggggggtg cgtcaggcgg cagcggatct ggaccggtcg ccaccatggt
     1861 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     1921 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     1981 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     2041 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     2101 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     2161 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     2221 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     2281 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     2341 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     2401 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     2461 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     2521 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaagcg gccgcgactc
     2581 tagatcataa tcagccatac cac
//
