LOCUS Exported 1065 bp ds-DNA linear SYN 13-JUN-2021 DEFINITION Localization: Nucleus, Excitation: 587, Emission: 610. ACCESSION . VERSION . KEYWORDS mCherry-Nucleus-7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1065) TITLE Davidson lab - mCherry constructs REFERENCE 2 (bases 1 to 1065) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1065 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 23..74 /label=CMVd2 promoter /note="truncated human cytomegalovirus (CMV) immediate early promoter that yields moderately reduced expression relative to the full-length promoter" primer_bind 25..45 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 113..122 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 119..826 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(264..282) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 532..551 /label=mCherry-F /note="mCherry, forward primer" CDS 851..871 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 875..895 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 899..919 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct 61 atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat 121 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt 181 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg 241 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt 301 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc 361 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt 421 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg 481 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat 541 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc 601 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga 661 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa 721 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg 781 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtccg gactcagatc 841 tcgagctgat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtagatcc 901 aaaaaagaag agaaaggtag gatccaccgg atctagataa ctgatcataa tcagccatac 961 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa 1021 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagc //