LOCUS Exported 7171 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Empty vector, integration at amyE, ampr, cmr. ACCESSION . VERSION . KEYWORDS pBS1C SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7171) AUTHORS Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T TITLE The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. JOURNAL J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29. PUBMED 24295448 REFERENCE 2 (bases 1 to 7171) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7171 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(1149..1168) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" rep_origin complement(3583..4171) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3663..3682) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4342..5202) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4965..4984 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5203..5307) /gene="bla" /label=AmpR promoter primer_bind 5375..5393 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" misc_feature 6065..6086 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" protein_bind 6187..6208 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6223..6253 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6261..6277 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 6295..6306 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 6313..6990 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" terminator 7035..7106 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 7122..7149 /label=T7Te terminator /note="phage T7 early transcription terminator" misc_feature 7156..5 /label=BioBrick suffix /note="universal suffix for all parts" ORIGIN 1 tgcagtccgg caaaaaaggg caaggtgtca attctcatgt ttgacagctt atcatcggca 61 atagttaccc ttattatcaa gataagaaag aaaaggattt ttcgctacgc tcaaatcctt 121 taaaaaaaca caaaagacca cattttttaa tgtggtcttt attcttcaac taaagcaccc 181 attagttcaa caaacgaaaa ttggataaag tgggatattt ttaaaatata tatttatgtt 241 acagtaatat tgacttttaa aaaaggattg attctaatga agaaagcaga caagtaagcc 301 tcctaaattc actttagata aaaatttagg aggcatatca aatgaacttt aataaaattg 361 atttagacaa ttggaagaga aaagagatat ttaatcatta tttgaaccaa caaacgactt 421 ttagtataac cacagaaatt gatattagtg ttttataccg aaacataaaa caagaaggat 481 ataaatttta ccctgcattt attttcttag tgacaagggt gataaactca aatacagctt 541 ttagaactgg ttacaatagc gacggagagt taggttattg ggataagtta gagccacttt 601 atacaatttt tgatggtgta tctaaaacat tctctggtat ttggactcct gtaaagaatg 661 acttcaaaga gttttatgat ttataccttt ctgatgtaga gaaatataat ggttcgggga 721 aattgtttcc caaaacacct atacctgaaa atgctttttc tctttctatt attccatgga 781 cttcatttac tgggtttaac ttaaatatca ataataatag taattacctt ctacccatta 841 ttacagcagg aaaattcatt aataaaggta attcaatata tttaccgcta tctttacagg 901 tacatcattc tgtttgtgat ggttatcatg caggattgtt tatgaactct attcaggaat 961 tgtcagatag gcctaatgac tggcttttat aatatgagat aatgccgact gtacttttta 1021 cagtcggttt tctaatgtca ctaacctgcc ccgttagttg aagaaggttt ttatattaca 1081 gctccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt 1141 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct 1201 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg 1261 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact 1321 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgacatg gatgagcgat 1381 gatgatatcc gtttaggctg ggcggtgata gcttctcgtt caggcagtac gcctcttttc 1441 ttttccagac ctgagggagg cggaaatggt gtgaggttcc cggggaaaag ccaaataggc 1501 gatcgcggga gtgctttatt tgaagatcag gctatcactg cggtcaatag atttcacaat 1561 gtgatggctg gacagcctga ggaactctcg aacccgaatg gaaacaacca gatatttatg 1621 aatcagcgcg gctcacatgg cgttgtgctg gcaaatgcag gttcatcctc tgtctctatc 1681 aatacggcaa caaaattgcc tgatggcagg tatgacaata aagctggagc gggttcattt 1741 caagtgaacg atggtaaact gacaggcacg atcaatgcca ggtctgtagc tgtgctttat 1801 cctgatgata ttgcaaaagc gcctcatgtt ttccttgaga attacaaaac aggtgtaaca 1861 cattctttca atgatcaact gacgattacc ttgcgtgcag atgcgaatac aacaaaagcc 1921 gtttatcaaa tcaataatgg accagagacg gcgtttaagg atggagatca attcacaatc 1981 ggaaaaggag atccatttgg caaaacatac accatcatgt taaaaggaac gaacagtgat 2041 ggtgtaacga ggaccgagaa atacagtttt gttaaaagag atccagcgtc ggccaaaacc 2101 atcggctatc aaaatccgaa tcattggagc caggtaaatg cttatatcta taaacatgat 2161 gggagccgag taattgaatt gaccggatct tggcctggaa aaccaatgac taaaaatgca 2221 gacggaattt acacgctgac gctgcctgcg gacacggata caaccaacgc aaaagtgatt 2281 tttaataatg gcagcgccca agtgcccggt cagaatcagc ctggctttga ttacgtgcta 2341 aatggtttat ataatgactc gggcttaagc ggttctcttc cccattgagg gcaaggctag 2401 acgggactta ccgaaagaaa ccatcaatga tggtttcttt tttgttcata aatcagacaa 2461 aacttttctc ttgcaaaagt ttgtgaagtg ttgcacaata taaatgtgaa atacttcaca 2521 aacaaaaaga catcaaagag aaacataccc tgcaaggatg ctgatattgt ctgcatttgc 2581 gccggagcaa accaaaaacc tggtgagaca cgccttgaat tagtagaaaa gaacttgaag 2641 attttcaaag gcatcgttag tgaagtcatg gcgagcggat ttgacggcat tttcttagtc 2701 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg 2761 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt 2821 caaggatcgc tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg 2881 gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc 2941 ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctactga 3001 agtggatttc tttaagagct cctttaactt cctcaccagt agttgtatcg gtaccataag 3061 tagaagcagc aacccaagta gctttaccag catccggttc aaccagcata gtaagaatct 3121 tactggacat cggcagttct tcgaacagtg cgccaactac cagctctttc tccagaatgg 3181 gctatacctc ttttacctaa agagcatgta actttactgg atatagctag aaaaggctat 3241 cggggagagt gtgatgataa gtgggaagga ctatattcaa aggtgaaagc actcgttaag 3301 tatatgaaaa attctataga aacttctctc aattaggcta attttattgc aataacaggt 3361 gcttactttt ctggagttct ttagcaaatt tttttattag ctgaacttag tattagtggc 3421 catactcctc caatccaaag ctatttagaa agattactat atcctcaaac aggcggtaac 3481 cggcctcttc atcgggaatg cgcgcgacct tcagcatcgc cggcatgtcc ccctggcgga 3541 cgggaagtat ccagctcgag gtcgggccgc gttgctggcg tttttccata ggctccgccc 3601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 3661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 3721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 3781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 3841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 3901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 3961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 4021 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttga 4081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 4141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 4201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 4261 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 4321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 4381 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 4441 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 4501 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 4561 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 4621 gccagttaat agtttgcgca acgttgttgc cattgctgcc ggcatcgtgg tgtcacgctc 4681 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 4741 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 4801 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 4861 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 4921 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 4981 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 5041 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 5101 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 5161 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 5221 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 5281 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 5341 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 5401 tcttcaagaa ttaacaaaat tctccagtct tcacatcggt ttgaaaggag gaagcggaag 5461 aatgaagtaa gagggatttt tgactccgaa gtaagtcttc aaaaaatcaa ataaggagtg 5521 tcaagaatgt ttgcaaaacg attcaaaacc tctttactgc cgttattcgc tggattttta 5581 ttgctgtttc atttggttct ggcaggaccg gcggctgcga gtgctgaaac ggcgaacaaa 5641 tcgaatgagc ttacagcacc gtcgatcaaa agcggaacca ttcttcatgc atggaattgg 5701 tcgttcaata cgttaaaaca caatatgaag gatattcatg atgcaggata tacagccatt 5761 cagacatctc cgattaacca agtaaaggaa gggaatcaag gagataaaag catgtcgaac 5821 tggtactggc tgtatcagcc gacatcgtat caaattggca accgttactt aggtactgaa 5881 caagaattta aagaaatgtg tgcagccgct gaagaatatg gcataaaggt cattgttgac 5941 gcggtcatca atcataccac cagtgattat gccgcgattt ccaatgaggt taagagtatt 6001 ccaaactgga cacatggaaa cacacaaatt aaaaactggt ctgatcggat cctagaagct 6061 tatcgaattc gcggccgctt ctagagcaat acgcaaaccg cctctccccg cgcgttggcc 6121 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 6181 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 6241 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagaaagag 6301 gagaaatact agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt 6361 cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt 6421 ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc 6481 gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg 6541 gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt 6601 atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt 6661 gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgatgg tccggttatg 6721 cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct 6781 ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa 6841 gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac 6901 atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt 6961 gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgcta 7021 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 7081 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 7141 ctttctgcgt ttatatacta gtagcggccg c //