LOCUS Exported 2133 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION 10xHis-tag. ACCESSION . VERSION . KEYWORDS His10-tag SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2133) AUTHORS Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T TITLE The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. JOURNAL J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29. PUBMED 24295448 REFERENCE 2 (bases 1 to 2133) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2133 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 46..75 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" misc_feature 85..105 /label=BioBrick suffix /note="universal suffix for all parts" terminator 106..163 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(187..204) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(358..946) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(438..457) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1129..1223 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1244..1903) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1818..1837 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 2065..2083 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2087..2130 /label=bacterial terminator /note="putative bacterial transcription terminator" ORIGIN 1 aattccgcgg ccgcttctag ataaggagga actactatgg ccggccatca tcatcatcat 61 catcatcatc atcataccgg ttaatactag tagcggccgc tgcagtccgg caaaaaaggg 121 caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca 181 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 361 ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 781 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 1021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 1081 aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat 1141 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat 1201 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc 1261 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa 1321 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt 1381 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac 1441 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag 1501 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact 1561 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga 1621 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca 1681 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa 1741 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct 1801 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt 1861 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc 1921 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa 1981 agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt 2041 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa 2101 aaatccttag ctttcgctaa ggatgatttc tgg //